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51 publications mentioning hsa-mir-532

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-532. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 308
Other miRNAs from this paper: hsa-mir-29a, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-498, hsa-mir-3064
Reversely, hTERT knockdown in S KOV-3 cells suppressed miR-532/miR-3064 inhibitors -induced Slug expression and increased the expression of Bax reduced by miR-532/miR-3064 inhibitors (Fig 4C, right panel). [score:12]
Lower: Western blot analysis was performed in S KOV-3 cells transfected with miR-532/miR-3064 inhibitors, together with (or without) hTERT siRNA; (B) (upper panel) and transwell invasion assay (lower panel) were performed in OC cells transfected as described above; (C) The protein expression of Slug and Bax in ES-2 (left panel) and S KOV-3 (right panel) cells transfected with miR-532/miR-3064 mimics (or miR-532/miR-3064 inhibitors) followed by hTERT overexpression (or hTERT knockdown). [score:9]
In contrast, the silencing of miR-532/miR-3064 with miRNA inhibitors resulted in the up-regulation of Slug and down-regulation of Bax and E-cadherin (Fig 2D). [score:9]
Neg mimic: negative control miRNA mimic; Neg inhibitor: negative control miRNA inhibitor; (C, D) Expression of hTERT, Slug, E-cadherin, Bax and GAPDH was determined using western blotting in ES-2 (C) or S KOV-3 (D) cells transfected with miR-532/miR-3064 mimics or miR-532/miR-3064 inhibitors, respectively; (E) The qPCR analysis of hTERT levels in OC patient samples and normal ovarian tissue samples; (F) Kaplan-Meier survival curves showing lower overall survival in patients with high (above median value) versus low (below median value) hTERT levels. [score:9]
This study shows that miR-532 and miR-3064 that were down-regulated in OC tissues, can suppress the proliferation, EMT and invasion of OC cells by directly repressing hTERT expression. [score:9]
To further confirm the role of hTERT in the function of miR-532/miR-3064 in OC cells, we examined the protein expression of Slug and Bax in OC cells transfected with miR-532/miR-3064 mimics (or miR-532/miR-3064 inhibitors) followed by hTERT overexpression (or hTERT knockdown). [score:8]
These data suggest a possibility that miR-532/miR-3064 acts directly to suppress hTERT expression to suppress the EMT process and induce apoptosis in OC cells. [score:8]
Since the overexpression of hTERT induces OC cell invasion via up -regulating Slug expression [5], and the silencing of hTERT in glioma cells decreased cell proliferation by increasing the protein levels of Bax [21], we evaluated whether the modulation of hTERT levels by miR-532/miR-3064 affects the protein expressions of Slug, E-cadherin (a downstream target of Slug) and Bax in OC cells. [score:8]
Consistently, overexpression of miR-532 and miR-3064 downregulated the expression of Ki-67, a cell proliferation marker (Fig 5B). [score:8]
On the other hand, the silencing of hTERT expression by specific siRNA oligonucleotides in the presence of miR-532/miR-3064 inhibitors could suppress the proliferation and invasion of S KOV-3 cells (Fig 4A and 4B). [score:7]
Moreover, high levels of hTERT were observed in ES-2 and S KOV-3 cells expressing decreased levels of miR-532/miR-3064, while a low level of hTERT was observed in NOEC cells (Fig 1H), suggesting that miR-532/miR-3064 expression inversely correlates with hTERT expression in OC cells, and the repression of these two miRNAs might be important for OC growth and/or progression. [score:7]
Interestingly, hTERT overexpression in ES-2 cells could increase the expression of Slug reduced by miR-532/miR-3064 mimic, and can also decrease the expression of Bax elevated by miR-532/miR-3064 mimic (Fig 4C, left panel). [score:7]
Together, these data suggest that miR-532/miR-3064 acts directly to suppress hTERT expression in OC cells. [score:6]
Here, we provided new evidence that the tumor suppressive roles of miR-532 in OC cells is mediated, at least in part, by the down-regulation of hTERT oncogene. [score:6]
When hTERT levels were down-regulated by the transfection of miR-532/miR-3064 mimics, Slug levels were decreased, and Bax and E-cadherin expression was induced (Fig 2C). [score:6]
In conclusion, our data suggest that hTERT overexpression might be caused by the loss of miR-532/miR-3064 (two direct suppressors of hTERT), and has a key role in enhancing OC cell proliferation and invasion. [score:6]
To ascertain whether hTERT is directly targeted by miR-532/miR-3064, we performed gain-of-function experiments using ES-2 cells that express relatively low levels of miR-532/miR-3064. [score:6]
Our in vivo experiments revealed that reduced levels of miR-532/miR-3064 correlate with hTERT up-regulation in OC tissues, and associate with worse OC patient survival, indicating that these two miRNAs could be causal factors in the overexpression of hTERT in OC. [score:6]
Furthermore, the western blotting analysis demonstrated that the transfection with miR-532/miR-3064 mimics reduced hTERT expression (Fig 2C), while the knockdown of miR-532/miR-3064 using miRNA inhibitors increased the levels of hTERT protein (Fig 2D). [score:6]
In S KOV-3 cells, the down-regulation of miR-532/miR-3064 by miRNA inhibitors led to a scattered morphology consistent with EMT (Fig 3B). [score:6]
60 OC patients were divided into two groups with higher (n = 30) or lower (n = 30) expression of miR-532 or miR-3064, using the median expression values of miR-532 or miR-3064 in OC samples. [score:5]
miR-532/miR-3064 overexpression inhibits, whereas miR-532/miR-3064 silencing promotes proliferation and invasion in OC cells. [score:5]
We found that the expression of miR-532/miR-3064 was significantly down-regulated in OC tissues compared with normal specimens (Fig 1B and 1C). [score:5]
The forced expression of the hTERT cDNA vector lacking the 3'-UTR sequence could recover hTERT protein expression in ES-2 cells transfected with miR-532/miR-3064 mimics (Fig 4A). [score:5]
To examine whether these interactions between miR-532/miR-3064 and hTERT 3'-UTR are direct, mutations targeting the predicted -binding sites of miR-532 or miR-3064 within the hTERT 3'-UTR were generated. [score:5]
Using target prediction programs (TargetScan and DIANA-MicroT-CDS), we found that hTERT contains seed sequences for two putative miRNAs (miR-532 and miR-3064) in its 3'-UTR. [score:5]
Kaplan-Meier survival curves for OC patients were plotted based on high or low miR-532 (F)/miR-3064 (G) expression; (H) Western blot analysis for the expression of hTERT or GAPDH in S KOV-3, ES-2 and NOEC cells. [score:5]
High Expression of miR-532-5p, a Tumor Suppressor, Leads to Better Prognosis in Ovarian Cancer Both In Vivo and In Vitro. [score:5]
Conversely, the transfection of inhibitors for miR-532 or miR-3064 to another OC cell line S KOV-3 expressing relatively high levels of miR-532/miR-3064 enhanced the luciferase activity of the WT hTERT 3'-UTR (Fig 2B). [score:5]
Collectively, these results support that hTERT inhibition is a key contributor of miR-532/miR-3064's tumor suppressive roles in OC cells. [score:5]
0173912.g001 Fig 1. (A) A schematic diagram explains base pairing between miR-532/3064 with the 3'-UTR sequences of hTERT; (B, C) qPCR analysis of miR-532 (B)/miR-3064 (C) levels in 60 OC patient samples and 20 normal ovarian tissue samples; (D, E) Endogenous expression of miR-532 (D)/miR-3064 (E) was determined using qPCRs in OC cell lines (S KOV-3 and ES-2) and normal ovarian epithelial NOEC cells; (F, G) OC patients were categorized into two groups with high (above median, n = 30) or low (below median, n = 30) miRNA expression. [score:5]
0173912.g003 Fig 3. (A, B) Cell morphology of OC cells transfected with either miR-532/miR-3064 mimics (A) or miR-532/miR-3064 inhibitors (B); (C, D) Representative images of invaded ES-2 (C) and S KOV-3 (D) cells transfected as indicated; (E, F) (E) and transwell invasion assay (F) with OC cells transfected with miR-532/miR-3064 mimics or miR-532/miR-3064 inhibitors. [score:4]
Our luciferase assays suggested that, the overexpression of either miR-532 or miR-3064 mimic markedly suppressed the luciferase activity of the wild-type (WT) version of hTERT 3'-UTR in ES-2 cells (Fig 2A). [score:4]
Our in vitro results further show that the direct inhibition of hTERT achieved by transient transfection of miR-532/miR-3064 mimics can repress OC cell proliferation and invasion. [score:4]
MicroRNA-532-5p suppresses cervical cancer cell growth by targeting hTERT. [score:4]
To understand the potential roles of miR-532/miR-3064 in regulating OC cell proliferation and invasion, we performed cell proliferation assay and in vitro cell invasion assay, and found that the ectopic expression of miR-532/miR-3064 strongly suppresses the proliferative and invasive capacity of OC cells (Fig 3C, 3E and 3F). [score:4]
Furthermore, we analyzed whether miR-532/miR-3064 expression levels correlate with human OC patient survival. [score:3]
Low expression of miR-532/miR-3064 is associated with poor survival of patients with OC. [score:3]
Lentiviral vectors (pEZX-MR03) for overexpression of miR-532/miR-3064 and the negative control lentiviral vector were purchased from Applied Biological Materials Inc. [score:3]
miR-532 and miR-3064 inhibits OC growth in a mouse mo del. [score:3]
ES-2 cells overexpressing miR-532 or miR-3064 exhibited attenuated tumorigenic ability 26 days after implantation (Fig 5A). [score:3]
To test the possibility that miR-532/miR-3064 suppresses OC cell proliferation and invasion, we transiently transfected ES-2 cells with miR-532/miR-3064 mimics and observed that elevating miR-532/miR-3064 levels induced a cellular phenotypic change from a fibroblastic mesenchymal morphology to a more rounded epithelial-like morphology (Fig 3A). [score:3]
In an OC xenograft mo del, overexpressing miR-532/miR-3064 significantly decreased tumor growth of OC cells in vivo. [score:3]
Importantly, “rescuing” hTERT expression in the presence of miR-532/miR-3064 mimics enhances the proliferation and invasion of ES-2 cells (Fig 4B). [score:3]
Using qPCR analysis, we detected an inverse relationship between the expression of miR-532/miR-3064 and hTERT mRNA levels in OC tissues (Fig 1B and 1C; Fig 2E). [score:3]
It is well established that one single miRNA could target multiple mRNAs, the other miR-532 targets in OC cells require further investigation. [score:3]
We further analyzed the expression of miR-532/miR-3064 in OC cell lines and normal ovarian epithelial NOEC cells. [score:3]
We screened OC patient clinical tissues (n = 60) and normal ovarian epithelial tissues (n = 20) for the endogenous expression of miR-532 and miR-3064 using qPCR. [score:3]
hTERT is a critical mediator of miR-532/miR-3064's tumor suppressive effects in OC cells. [score:3]
Our analysis revealed lower levels of both miR-532 and miR-3064 in ES-2 and S KOV-3 cells than that in NOEC cells (Fig 1D and 1E), indicating that these two miRNAs are potential tumor suppressors in OC. [score:3]
Previous studies have reported that higher miR-532 expression correlates with longer survival of OC patients [26]. [score:3]
Identification of hTERT as a target for miR-532 and miR-3064 in OC cells. [score:3]
However, the transfection with mimics or inhibitors for miR-532/miR-3064 did not significantly influence the luciferase activity of the mutated version of hTERT 3'-UTR in OC cells (Fig 2A and 2B). [score:3]
These data suggest that increasing miR-532 and miR-3064 levels can suppress the growth of OC cells in vivo. [score:3]
miR-532 and miR-3064 inhibit OC growth in vivo. [score:3]
Kaplan-Meier analysis demonstrated that reduced expression of either miR-532 or miR-3064 was significantly associated with poorer prognosis in patients with OC (Fig 1F and 1G). [score:3]
Therefore, we selected miR-532 and miR-3064 as our experimental targets. [score:3]
Transient overexpression of miR-532 and miR-3064 resulted in significant repression of hTERT mRNA in human OC cell lines (ES-2 and S KOV-3) (data not shown). [score:3]
Re -expression of miR-532 or miR-3064 might be a possible strategy for the treatment of OC. [score:3]
Lentiviral overexpression of miR-532 and miR-3064. [score:3]
0173912.g002 Fig 2. (A, B) Luciferase assay was performed in OC cells that were co -transfected with reporter vectors carrying either the wild-type (WT) version or the mutated (MUT) version of hTERT 3'-UTR, along with either miR-532/miR-3064 mimics (A) or miR-532/miR-3064 inhibitors (B). [score:2]
Predicted miR-532- or miR-3064 -binding sites were mutated by RiboBio Co. [score:1]
0173912.g004 Fig 4. (A) Upper: western blot analysis of hTERT and GAPDH levels in ES-2 cells after transfection with miR-532/miR-3064 mimics, along with (or without) hTERT cDNA vector (ORF) lacking the 3'-UTR region. [score:1]
To delineate the clinical significance of miR-532 or miR-3064, we determined the correlations between the levels of miR-532/miR-3064 and clinicopathological factors. [score:1]
However, our knowledge of the molecular mechanisms mediating miR-532's function in OC specifically, has been limited. [score:1]
To evaluate the effects of miR-532 and miR-3064 on tumor growth in vivo, we manipulated the expression levels of miR-532 and miR-3064 in ES-2 cells, and then injected ES-2 cells into the flanks of nude mice to establish subcutaneous OC xenografts. [score:1]
Association between miR-532/miR-3064 expression and clinicopathological characteristics of epithelial ovarian cancer. [score:1]
In contrast, the silencing of miR-532 or miR-3064 stimulates OC cell proliferation and invasion (Fig 3D, 3E and 3F). [score:1]
More importantly, lower levels of miR-532/miR-3064 were significantly associated with advanced tumor stage, higher tumor grade and higher incidence of lymph node metastasis (S1 Table). [score:1]
Given the oncogenic roles of hTERT in promoting OC cell proliferation and invasion [5, 6], we next determined whether hTERT serves as a critical mediator of miR-532/miR-3064's roles in cellular proliferation and invasiveness. [score:1]
ES-2 cells were infected with lentivirus and selected using 1 μg/ml puromycin for 4 weeks, to establish stable miR-532, miR-3064 or negative control (Neg) transfectants. [score:1]
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2
[+] score: 35
Other miRNAs from this paper: hsa-mir-660, hsa-mir-663b
Additional file 21: Differentially expressed target genes of hsa-miR-660 and hsa-miR-532. [score:5]
Excel file showing the differentially expressed target genes of hsa-miR-660 and hsa-miR-532 between female and male human islets. [score:5]
We next used TargetScan Human Release 6.2 (June 2012) [53] to find potential target genes of hsa-miR-660 and hsa-miR-532 (Additional files 19 and 20). [score:5]
Although no previous study has linked these microRNAs to islet function, we found sex differences in islet expression of six target genes for hsa-miR-660 and hsa-miR-532 (for example, XPOT and SPIRE1). [score:5]
Excel file showing potential target genes of hsa-miR-532 generated using TargetScan Human release 6.2 (June 2012) [53]. [score:5]
Additional file 20: Target genes for hsa-miR-532. [score:3]
Differential methylation between sexes is associated with altered levels of microRNAs miR-660 and miR-532 and related target genes. [score:3]
Two of these microRNAs, hsa-mir-660 and hsa-miR-532, also showed elevated expression in parallel with decreased DNA methylation in female compared with male islets. [score:2]
We found two microRNAs located on the X chromosome, hsa-miR-660 and hsa-miR-532, that exhibited lower DNA methylation and higher expression levels in pancreatic islets from female compared with male donors (Figure  7). [score:2]
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3
[+] score: 32
org) we identified nine miRNAs that regulate these three genes: miR-368 targeting MINT31, miR-181d, miR-30a-3p, miR-30c, miR-30d, miR-30e-3p, miR-370, miR-493-5p and miR-532-5p targeting CDH13, and miR-181d targeting RASSF1. [score:8]
Expression of miR-30c (P = 0.01), miR-30d (P = 0.002), miR-30e-3p (P = 0.008) and miR-532-5p (P = 0.002) were significantly downregulated in Her2/neu -positive ovarian carcinomas (Figure 3). [score:6]
Expression of miR-30c, miR-30d, miR-30e-3p and miR-532-5p was significantly downregulated among Her2/neu -positive ovarian carcinomas. [score:6]
Finally, lower expression of miR-30c, miR-30d, miR-30e-3p and miR-532-5p was significantly associated with overexpression of Her-2/neu. [score:5]
Expression of miR-532-5p was significantly lower in borderline than in benign tissues. [score:3]
In addition, expression of miR-532-5p (P = 0.01) was lower in borderline than in benign neoplasms. [score:3]
An additional three miRNAs (miR-181d, miR-30a-3p, miR-532-5p) were significantly different between borderline and carcinoma tissues. [score:1]
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4
[+] score: 27
Other miRNAs from this paper: hsa-mir-145, hsa-mir-126, hsa-mir-150, hsa-mir-195, hsa-mir-582
Figure 1The expression of miR-582, miR-195 and miR-532 under normoxic and hypoxic conditions as detected by qRT-PCR (* P<0.05) Knockdown of miR-195 promotes the autophagy of hEPCs under hypoxiaSince the expression level of miR-195 is increased under hypoxia, we transfected a miR-195 inhibitor into hEPCs and the cells were harvested for qRT-PCR. [score:8]
Figure 1The expression of miR-582, miR-195 and miR-532 under normoxic and hypoxic conditions as detected by qRT-PCR (* P<0.05) Since the expression level of miR-195 is increased under hypoxia, we transfected a miR-195 inhibitor into hEPCs and the cells were harvested for qRT-PCR. [score:7]
However, the expression and function of miR-582, miR-195 and miR-532 in EPCs, especially in hypoxic conditions such as that of DVT, remains unknown. [score:3]
The expression of miR-582, miR-195 and miR-532 under normoxic and hypoxic conditions as detected by qRT-PCR (* P<0.05). [score:3]
The results of qRT-PCR showed that the expression of miR-582, miR-195 and miR-532 was all significantly increased under conditions of hypoxia compared with that under normoxia (Figure 1). [score:2]
To analyse miR-582, miR-195 and miR-532 expression, reverse transcription PCR was performed using specific stem-loop reverse transcription primers, miRNA first strand synthesis was performed using a First Strand Synthesis Kit (Takara), and quantitative real-time PCR analysis (qRT-PCR) was performed using a SYBR Green Real time PCR Master Mix (Toyobo) on an Applied Biosystems 7500 system (Applied Biosystems). [score:2]
In the present study, we firstly investigated the expression level of miR-582, miR-195 and miR-532 in hEPCs under hypoxic conditions. [score:1]
In another previous study, a panel of miRNAs, such as miR-582, miR-195 and miR-532, were identified as new biomarkers for the detection of DVT [14]. [score:1]
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5
[+] score: 25
While expression of some of these miRNAs seemed to be specifically dysregulated in certain tumor stages, 12 miRNAs, including 4 up-regulated (miR-200c, miR-487a, miR-491-3p and miR-452) and 8 down-regulated miRNAs (miR-125b, miR-142-3p, miR-199a-5p, miR-22, miR-299-3p, miR-29a, miR-429, and miR-532-5p), were identified to be commonly dysregulated in all ccRCC tumors at different stages (Table 1). [score:11]
Eleven commonly dysregulated miRNAs, including 3 up-regulated (miR-487a, miR-491-3p and miR-452) and 8 down-regulated (miR-125b, miR-142-3p, miR-199a-5p, miR-22, miR-299-3p, miR-29a, miR-429, and miR-532-5p), were identified in ccRCC tumor samples as compared with adjacent nontumorous samples. [score:7]
Eleven miRNAs were identified to be commonly dysregulated, including three up-regulated (miR-487a, miR-491-3p and miR-452) and eight down-regulated (miR-125b, miR-142-3p, miR-199a-5p, miR-22, miR-299-3p, miR-29a, miR-429, and miR-532-5p) in tumor tissues as compared with adjacent normal tissues. [score:7]
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6
[+] score: 22
miRNA expression analysis by RNAseq, qRT-PCR, and Northern blot revealed that three host miRNAs were significantly up-regulated during infection (miR-1271-5p, miR-532-5p and miR-1307-3p). [score:6]
To identify possible miR-532-5p targets in human genes, the whole genome was evaluated by computational prediction assessing several features, such as evolutionary conservation of sites (miRDB, TargetScanHuman), seed region complementarity (Diana Micro-T, micrroRNA. [score:3]
These results suggested that miR-532-5p has a host target. [score:3]
Opposing results were observed when the cells were transfected with the miR-532-5p inhibitor. [score:3]
Finally, the luciferase reporter assays confirmed targeting of the TAB3 and SESTD1 3′ UTRs by miR-532-5p. [score:2]
The interaction between miR-532-5p and its targets (TAB3 and SESTD1) was further validated by Western blot, use of miR-532-5p mimics and luciferase assays. [score:2]
In conclusion, these results indicate that WNV infection induces cellular miR-532-5p, which in turn affects the host antiviral response [121]. [score:1]
Similar results were observed in non-infected cells treated with a miR-532-5p mimic. [score:1]
Only in the cells transfected with the miR-532-5p mimic was viral titre significantly reduced. [score:1]
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7
[+] score: 19
The miRWalk database also contains experimentally validated target genes for the additional three miRNAs (miR-1224-3p, miR-197 and miR-532-3p) that were differentially overexpressed in response to both proanthocyanidin extracts (Table 5), although the number of target genes for these miRNAs was much lower than for miR-30b*. [score:7]
On the other hand, the two procyanidin extracts upregulated the expression of miR-1224-3p, miR-197 and miR-532-3p. [score:6]
miR-1224-3p, miR-197 and miR-532-3p were differentially expressed after treatment with the two procyanidin extracts; however, only miR-30b* was differentially expressed in response to all the three treatments. [score:5]
has-miR-532-3p RUNX3 Transcription factor. [score:1]
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8
[+] score: 15
The ANOVA test with post hoc analysis for condition IL-4 vs conditions Pre and Ctrl identified 7 mature miRNAs regulated by IL-4 in CLL (Fig 2A and S6 Table): miR-21-3p, miR-362-3p, miR-362-5p, miR-500a-3p, miR-502-3p, miR-532-3p, and miR-532-5p, all of them higher than 10-fold up-regulated on average. [score:5]
We have found up-regulation of miR-21 (5p and 3p), miR-362 (3p and 5p), miR-500a-3p, miR-502-3p, and miR-532 (3p and 5p) by IL-4 in CLL. [score:4]
MiR-21, miR-362, miR-500a, miR-502, and miR-532 were induced by IL-4, likely as a consequence of up-regulation of their respective host genes, vacuole membrane protein 1 (VMP1), and chloride channel, voltage sensitive 5 (CLCN5). [score:4]
For miRNA expression, RNA samples were retrotranscribed with the miScript II RT Kit (Qiagen) using the miScript HiSpec buffer for mature miRNA detection only, followed by qPCR with the miScript SYBR Green PCR Kit (Qiagen) in an ABI Prism 7000 Sequence Detection System, using the miScript primer assays (Qiagen) for miR-21-3p, miR-362-3p, miR-362-5p, miR-500a-3p, miR-502-3p, miR-532, miR-532-3p, and RNU6-6P, the latter used as reference for normalization (cat. [score:1]
MiR-21 maps several hundred base pairs downstream of the last exon of VMP1, and miR-362, miR-500a, miR-502, and miR-532 within the third intron of CLCN5. [score:1]
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9
[+] score: 15
Among the 87 most-variable expressed miRNAs across the entire panel, a group of 15 miRNAs (hsa-miR-130a, hsa-miR-886-5p, hsa-miR-886-3p, hsa-miR-222, hsa-miR-21*, hsa-miR-29a, hsa-miR-23a, hsa-miR-24, hsa-miR-30a, hsa-miR-27a, hsa-miR-22, hsa-miR-532-3p, hsa-miR-100, hsa-miR-125b, hsa-miR-221) was significantly higher expressed in the minor cluster as opposed to other miRNAs (Figure 2, top red box). [score:5]
Notably, however, five of these six miRNAs (hsa-miR-141, hsa-miRNA-26a, hsa-miR-29c, hsa-miR-148b, hsa-miR-193a-3p) showed significantly higher expression in both ER -positive cell lines and primary tumors, and one miRNA (hsa-miR-532-3p) showed significantly lower expression in both ER -positive cell lines and ER -positive tumors (see Table S4B in Additional file 1). [score:5]
Similarly, the most highly expressed miRNAs in normal-like/claudin-low cell lines were hsa-miR-22, hsa-miR-532-3p, hsa-miR-125b, hsa-miR-501-5p, and hsa-miR-155*, whereas in basal-like cell lines miRNAs of the miR-200 family (hsa-miR-492, hsa-miR-26b, hsa-miR-617, hsa-miR-155) were highly expressed (fold change ≥ 2) (see Table S9 in Additional file 1). [score:5]
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10
[+] score: 14
Moreover, miR-130b [99], miR-301a [100], miR-106a [101], miR-103a [102], miR-495 [103], and miR-532-5p [104] directly inhibit RUNX3 translation at the post-transcriptional level. [score:6]
In addition, increased H3K9 dimethylation and reduced H3 acetylation, as well as the increased miR-130b, miR-301a, miR-106a, miR-103a, miR-495, and miR-532-5p, synergistically inhibited the expression of RUNX3 Numerous studies have demonstrated that H. pylori infection is closely related to abnormal CpG island methylation. [score:5]
In addition, increased H3K9 dimethylation and reduced H3 acetylation, as well as the increased miR-130b, miR-301a, miR-106a, miR-103a, miR-495, and miR-532-5p, synergistically inhibited the expression of RUNX3 The exploitation of characteristic epigenetic alterations during the malignant transformation of gastric mucosa allows for the prevention, diagnosis, treatment, and prognostic evaluation of gastric cancer from a new perspective independent of protein expression. [score:3]
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11
[+] score: 13
Although neither algorithm identified human endoglin mRNA MRE target sites for HITS-CLIP validated miRNAs (miR-20a-3p, miR-23b-5p, miR-29a-5p, miR-103-3p, miR-107, and miR-532-5p) (Table 7 (Tab. [score:3]
In contrast, miR-23b-5p, miR-522-3p, and miR-532-5p (experimentally cataloged miRNAs which target human endoglin mRNAs, Table 7 (Tab. [score:3]
Again, it is not clear why the Diana-microT-CDS and TargetScan algorithms did not identify the miR-20a-3p, miR-23b-5p, miR-29a-5p, miR-103-3p, miR-107 and miR-532-5p MREs in human S-endoglin mRNA that were detected manually. [score:3]
7) (References in Table 7: let-7b-5p: Selbach et al., 2008[145]; miR-16-5p: Balakrishnan et al., 2014[14]; miR-20a-3p: Balakrishnan et al., 2014[14]; miR-23b-5p: Balakrishnan et al., 2014[14]; miR-29a-5p: Balakrishnan et al., 2014[14]; miR-103a-3p: Balakrishnan et al., 2014[14]; miR-107: Balakrishnan et al., 2014[14]; miR-532-5p: Haecker et al., 2012[63]; miR-628-5p: Balakrishnan et al., 2014[14]; miR-522-3p: Tan et al., 2014[153]) documents ten human experimentally supported miRNA/endoglin mRNA interactions, and the methodology utilized to substantiate the interaction, the tissue and/or cell line used for experimentation, the location of the MRE if known, the type of interaction (direct or indirect), and the literature reference. [score:3]
It was also observed that two putative miR-532-5p CDS MREs (5′ AGGCAU 3′ and 5′ GGCAUG 3′, 6mer “seed” regions) located 247 and 1236 nts downstream from the start codon. [score:1]
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12
[+] score: 13
Notably, 5 downregulated miRNAs miR-532 and miR-362, miR-500, miR-501, miR-502 are clustered and together encoded in one intron of a renal specific gene voltage-gated chloride ion channel CLCN5, and have been reported to be downregulated in ccRCC [26]. [score:7]
confirmed overexpression of miR-21, miR-122 and miR-210 and downregulation of miR-199 and miR-532 (Figure 4). [score:6]
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13
[+] score: 13
In the main study plasma from the 77 subjects were analysed for expression of 12 miRNAs (let-7a, let-7f, miR-19a, miR-22, miR26a, miR28-5p, miR-99b, miR151-5p, miR-221, miR-532-3p, miR-548-3p, miR-766). [score:3]
At baseline we found a significant but weak correlation between miR-532-3p expression and serum 25(OH)D that was most probably due to chance. [score:3]
At baseline there was a significant and positive correlation between serum 25(OH)D and miR-532-3p expression (r = 0.24, P = 0.04). [score:3]
At baseline there was a significant and positive correlation between serum 25-hydroxyvitamin D and miR-532-3p expression (r = 0.24, P = 0.04). [score:3]
72(−1.12, -0.49)−0.79(−0.97, -0.48)−0.04(−0.19, 0.25)miR-2210.03(−0.32, 0.29)0.09(−0.21, 0.27)0.13(−0.21, 0.29)0.02(−0.27, 0.26)−0.28(−0.58, 0.10)−0.32(−0.53, 0.10)miR-26a2.07(1.90, 2.24)2.09(1.81, 2. 25)−0.02(−0.29, 0.26)1.94(1.70, 2.11)1.83(1.53, 2.15)−0.05(−0.39, 0.25)miR-28-5p−4.10(−4.51, -3.66)−4.11(−4.48, -3.71)0.09(−0.60, 0.53)−4.14(−4.63, -3.93)−4.30(−4.55, -3.95)−0.14(−0.76, 0.32)miR-532-3p−4.80(−5. [score:1]
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[+] score: 12
Further evidence of the distinct hESC and hMSC HRM profiles emerged via miRNA clusters- miR-379/656, mir-532/502, miR-17/92 and its paralog miR-106b/25 being downregulated by hypoxia in hESCs while conversely up-regulated in hMSCs. [score:7]
In the same manner, 25 up-regulated miRNAs lay within 9 miRNA clusters in hMSCs while 7 members of the miR-379/656cluster and 3 members of the miR-532/502 cluster showed up regulation (Fig 3b). [score:5]
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[+] score: 11
Interestingly, the expression level of the tumor suppressor gene RUNX3 was found to be inversely correlated with that of miR-532-5p from the miR-532-502 cluster in primary melanomas [20]. [score:5]
miR-532-5p (Figure  2B; mean fold change 2.4), miR-188-3p (Figure  2C; mean fold change 2.5), miR-362-5p (Figure  2D; mean fold change 4.0), miR-501-3p (Figure  2E; mean fold change 5.3), miR-660-3p (Figure  2F; mean fold change 2.2), and miR-502-5p (Figure  2G; mean fold change 3.0) were all markedly up-regulated in the triple -negative breast cancers compared to the normal breast tissue controls. [score:3]
Relative expression levels of (B) miR-532-5p, (C) miR-188-3p, (D) miR-362-5p, (E) miR-501-3p, (F) miR-660-3p, and (G) miR-502-5p in triple -negative breast cancer tissues (n = 19) and adjacent normal tissues (n = 4) are shown. [score:3]
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[+] score: 10
Furthermore, the down-regulation of miR-10a (UVA) and miR-532-5p (UVB) was confirmed. [score:4]
31 miR-411 –3.3 1.30E-07 14q32.31miR-494 [†] –2.0 1.90E-12 14q32.31 miR-503 –4.0 1.50E-15 Xq26.3 miR-532-5p –4.1 4.80E-19 Xp11.23miR-598 [†] –1.9 5.80E-10 8p23.1 miR-660 –2.4 1.60E-07 Xp11.23 Of the UV-regulated miRNAs 10 were found to be regulated by both UVA and UVB. [score:3]
31 miR-411 –3.3 1.30E-07 14q32.31miR-494 [†] –2.0 1.90E-12 14q32.31 miR-503 –4.0 1.50E-15 Xq26.3 miR-532-5p –4.1 4.80E-19 Xp11.23miR-598 [†] –1.9 5.80E-10 8p23.1 miR-660 –2.4 1.60E-07 Xp11.23Of the UV-regulated miRNAs 10 were found to be regulated by both UVA and UVB. [score:3]
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[+] score: 7
The top 8 downregulated (hsa-miR-200c, hsa-miR-212, hsa-miR-29a, hsa-miR-532, hsa-miR-141, hsa-miR-1, hsa-miR-363, hsa-miR-187) and 8 upregulated (hsa-miR-487, hsa-miR-452, hsa-miR-1233, hsa-miR-92a, hsa-miR-106b, hsa-miR-1290, hsa-miR-320, hsa-miR-26a) miRNAs were presented in Figure 1A. [score:7]
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[+] score: 7
In agreement with our expression direction, Zhou et al. [48] reported down-regulation of miR-423-3p and miR-532-3p in ischemia–reperfusion injury heart grafts. [score:7]
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[+] score: 7
However, Sp110 upregulated miR-155, miR-342, miR-3470a, miR-532 and miR-690. [score:4]
Of note, in both uninfected and Mtb-infected macrophages, Sp110 induced miR-155, miR-342, miR-3470a and miR-532, but inhibited let-7e, miR-1249, miR-125a, miR-132, miR-152, miR-16-1, miR-182, miR-183, miR-23a, miR-28a, miR-5114, miR-99a and miR-99b. [score:3]
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[+] score: 6
Wei Z et al. 27 found that hsa-miR-577, hsa-miR-1, hsa-miR-532-3p and hsa-miR-627 could significantly down-regulate the translation efficiency of CYP3A4 mRNA in the liver. [score:6]
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[+] score: 6
Although another candidate miRNA, miR-532, was able to arrest the cell cycle and significantly inhibit proliferation (Fig. 7C, Suppl. [score:3]
However, miR-532 did increase HBsAg expression in HepG2.2.15 cells (Suppl. [score:3]
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[+] score: 6
The level of expression of different miRNAs in many clusters displayed variable expression as exemplified by the cluster containing miR-532 and 99b (Figure 5). [score:5]
[A] miRNAs belonging to cluster miR-532, [B] cluster miR-99b and [C] cluster miR-106b in normal PBMC, K562 and HL60 cell lines. [score:1]
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[+] score: 5
Ben-Hamo et al. [73] found that breast cancer patients target the GATA3 pathway via hsa-miR-532 whereas GATA3 regulates hormone-sensitive breast cancer phenotype. [score:4]
The first 20 associations were all confirmed and only 2 of the first 40 MiRNAs were unconfirmed which are hsa-mir-30e ranked 23rd and hsa-mir-532 ranked 40th. [score:1]
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[+] score: 4
Down-regulation of Dicer was followed by decreased production of several miRNAs (miR1301, miR1249, miR1227, miR532-3p, miR625, miR1827, miR324-5p) as assessed by real-time PCR (data not shown). [score:4]
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[+] score: 4
The top five downregulated were: miR-122 (312-fold), Let-7g (204-fold), miR-103a (83-fold), miR-532 (79-fold), and miR-451a (62-fold). [score:4]
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[+] score: 4
001 hsa-miR-210 11p15.5 Up (3.23) 0.021 hsa-miR-30b 8q24.2 Down (-2.02) 0.015 hsa-miR-30c 1p34.2 Down (-2.32) 0.039 hsa-miR-494 14q32.3 Up (8.03) 0.021 hsa-miR-497 17p13.1 Down (-2.15) 0.014 hsa-miR-502 Xp11.23 Down (-2.43) 0.014 hsa-miR-532 Xp11.23 Down (-2.11) 0.049 hsa-miR-551b 3q26.2 Down (-7.30) 0.034 hsa-miR-622 13q31.1 Up (2.39) 0.015 Hierarchical clustering of 44 miRNA genes with significantly different expression (p<0.05) in tumor tissues. [score:3]
001 hsa-miR-210 11p15.5 Up (3.23) 0.021 hsa-miR-30b 8q24.2 Down (-2.02) 0.015 hsa-miR-30c 1p34.2 Down (-2.32) 0.039 hsa-miR-494 14q32.3 Up (8.03) 0.021 hsa-miR-497 17p13.1 Down (-2.15) 0.014 hsa-miR-502 Xp11.23 Down (-2.43) 0.014 hsa-miR-532 Xp11.23 Down (-2.11) 0.049 hsa-miR-551b 3q26.2 Down (-7.30) 0.034 hsa-miR-622 13q31.1 Up (2.39) 0.015 Three miRNAs (hsa-miR-494, hsa-miR-551b, and ebv-miR-BART19) were validated in an independent sample set of non-TRU- and TRU-type lung adenocarcinoma and corresponding normal lung tissue (n = 21 and 12, respectively) by qRT-PCR. [score:1]
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[+] score: 4
Specifically, miR-1182, miR-1207-5p, miR-1266, miR-532 and miR-3064, which bind within the h TERT 3’UTR, are downregulated and associated with a poor clinical outcome in bladder, gastric and ovarian cancer [169– 171]. [score:4]
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[+] score: 4
And then we detected the expression level changes of six miRNAs (miR-29a-3p, miR-22-3p, miR-199a-5p, miR-532-5p, 216 miR-142-3p, and miR-125b-5p) between normal and ccRCC samples by RT-PCR. [score:3]
cn/), six miRNAs (miR-29a-3p, miR-22-3p, miR-199a-5p, miR-532-5p, miR-142-3p, and miR-125b-5p) that could interact with lncRNA-H19 were predicted (Fig.   2a). [score:1]
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[+] score: 3
Conversely, both screens identified miR-532 as a miRNA that inhibits infection. [score:3]
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[+] score: 3
The rest of co-expressed clusters were listed for regions at 6q13 (including mir-30a and mir-30c-2), Xp11.23 (including mir-362, mir-500, mir-501, mir-502 and mir-532), 14q32.2 (including mir-134, mir-379 and mir-382), 14q32.31 (including mir-127, mir-432 and mir-770), 9q22.32 (including let-7d, mir-23b and mir-27b) and 7q22.1 (including mir-93 and mir-106b) (Table 3). [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-23a, hsa-mir-25, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-33a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-93, hsa-mir-96, hsa-mir-99a, hsa-mir-100, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-16-2, hsa-mir-198, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-10a, hsa-mir-10b, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-204, hsa-mir-210, hsa-mir-212, hsa-mir-181a-1, hsa-mir-214, hsa-mir-215, hsa-mir-216a, hsa-mir-217, hsa-mir-218-1, hsa-mir-218-2, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-mir-224, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-27b, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-128-1, hsa-mir-130a, hsa-mir-132, hsa-mir-135a-1, hsa-mir-135a-2, hsa-mir-142, hsa-mir-145, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-126, hsa-mir-134, hsa-mir-146a, hsa-mir-150, hsa-mir-186, hsa-mir-188, hsa-mir-193a, hsa-mir-194-1, hsa-mir-320a, hsa-mir-155, hsa-mir-181b-2, hsa-mir-128-2, hsa-mir-194-2, hsa-mir-106b, hsa-mir-29c, hsa-mir-219a-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-99b, hsa-mir-130b, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-362, hsa-mir-369, hsa-mir-375, hsa-mir-378a, hsa-mir-382, hsa-mir-340, hsa-mir-328, hsa-mir-342, hsa-mir-151a, hsa-mir-148b, hsa-mir-331, hsa-mir-339, hsa-mir-335, hsa-mir-345, hsa-mir-196b, hsa-mir-424, hsa-mir-425, hsa-mir-20b, hsa-mir-451a, hsa-mir-409, hsa-mir-484, hsa-mir-486-1, hsa-mir-487a, hsa-mir-511, hsa-mir-146b, hsa-mir-496, hsa-mir-181d, hsa-mir-523, hsa-mir-518d, hsa-mir-499a, hsa-mir-501, hsa-mir-487b, hsa-mir-551a, hsa-mir-92b, hsa-mir-572, hsa-mir-580, hsa-mir-550a-1, hsa-mir-550a-2, hsa-mir-590, hsa-mir-599, hsa-mir-612, hsa-mir-624, hsa-mir-625, hsa-mir-627, hsa-mir-629, hsa-mir-33b, hsa-mir-633, hsa-mir-638, hsa-mir-644a, hsa-mir-650, hsa-mir-548d-1, hsa-mir-449b, hsa-mir-550a-3, hsa-mir-151b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-454, hsa-mir-320b-2, hsa-mir-378d-2, hsa-mir-708, hsa-mir-216b, hsa-mir-1290, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, hsa-mir-378b, hsa-mir-3151, hsa-mir-320e, hsa-mir-378c, hsa-mir-550b-1, hsa-mir-550b-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-219b, hsa-mir-203b, hsa-mir-451b, hsa-mir-499b, hsa-mir-378j, hsa-mir-486-2
Differential expression of miR-18a, miR-532, miR-218, miR-625, miR-193a, miR-638, miR-550 and miR-633 can be used as a marker to predict prednisone response in pediatric ALL patients [76]. [score:3]
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[+] score: 3
In addition to being in the list of 18 miRNAs identified to be differentially expressed in patients with CRPS, hsa-miR-532-3p was associated with CRPS type, pain level, IL1Ra, and VEGF (Table 3 and Additional file 1). [score:3]
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[+] score: 3
In addition, expression patterns of miR-574-3p, miR-574-5p, miR-744*, miR-30a, miR-30d, miR-205 and miR-532-3p are also inconsistent with our results 29. [score:3]
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[+] score: 3
Among the most prominently expressed miRNAs were miR-10a, miR-152, miR-22, miR-26a/b, miR-29b, miR-30b/c, miR-345, and miR-532-5p. [score:3]
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[+] score: 3
We found several combinations of miRNAs including miRNA-155, miRNA-181 and miRNA-652 as well as miRNA-363, miRNA-532 and miRNA-582 which clustered or overlapped in their binding locations on these UTRs, suggesting possible competition for binding to control gene expression in a combinatorial fashion. [score:3]
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[+] score: 3
MiRNAs with expression profiles closest to the mean were miR-103, miR-185, miR-532-3p, miR-194, miR-126, miR-155, let-7e, miR-345, miR-425 and miR-15b as illustrated in Table 3. The first 9 miRNAs on this list were excluded from further EC analysis on the basis of their documented roles in breast cancer (Table 3). [score:3]
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[+] score: 3
Additionally, prior studies from various mo dels of retinal degeneration identified over 300 differentially expressed miRNAs 63– 90, a total of 16 common miRNAs were identified (miR-1187, miR-125b-5p, miR-331-3p, miR466d-3p, miR-467f, miR-542-3p, miR-574-5p, miR654-3p, miR669h-3p, miR-882, miR-342-3p, miR-466a-5p, miR-466d-5p, miR-706, miR-345-3p, miR532-5p). [score:3]
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[+] score: 2
Other miRNAs from this paper: hsa-mir-17, hsa-mir-28, hsa-mir-223, hsa-mir-127, hsa-mir-188, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-30e, hsa-mir-362, hsa-mir-363, hsa-mir-367, hsa-mir-379, hsa-mir-196b, hsa-mir-450a-1, hsa-mir-431, ssc-mir-28, hsa-mir-493, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-500a, hsa-mir-501, hsa-mir-502, hsa-mir-450a-2, hsa-mir-513a-1, hsa-mir-513a-2, hsa-mir-506, hsa-mir-508, hsa-mir-509-1, hsa-mir-615, hsa-mir-660, bta-mir-127, bta-mir-30e, bta-mir-17, bta-mir-450a-2, bta-mir-532, bta-mir-363, bta-mir-660, hsa-mir-891a, hsa-mir-892a, hsa-mir-509-2, hsa-mir-450b, hsa-mir-892b, hsa-mir-708, hsa-mir-509-3, hsa-mir-1285-1, hsa-mir-1285-2, hsa-mir-1248, ssc-mir-17, bta-mir-155, bta-mir-188, bta-mir-194-2, bta-mir-196b, bta-mir-223, bta-mir-28, bta-mir-362, bta-mir-367, bta-mir-379, bta-mir-431, bta-mir-493, bta-mir-500, bta-mir-502a-1, bta-mir-502a-2, bta-mir-502b, bta-mir-615, bta-mir-708, bta-mir-1248-1, bta-mir-1248-2, ssc-mir-450a, bta-mir-2320, bta-mir-1388, bta-mir-194-1, bta-mir-450a-1, eca-mir-30e, eca-mir-367, eca-mir-684, eca-mir-196b, eca-mir-615, eca-mir-708, eca-mir-194-1, eca-mir-493a, eca-mir-17, eca-mir-1248, eca-mir-28, eca-mir-127, eca-mir-379, eca-mir-431, eca-mir-493b, eca-mir-155, eca-mir-194-2, eca-mir-188, eca-mir-223, eca-mir-362, eca-mir-363, eca-mir-450a, eca-mir-450b, eca-mir-450c, eca-mir-500-1, eca-mir-500-2, eca-mir-501, eca-mir-502, eca-mir-508, eca-mir-509a, eca-mir-532, eca-mir-660, ssc-mir-30e, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-127, ssc-mir-532, ssc-mir-708, ssc-mir-1285, ssc-mir-500, hsa-mir-514b, ssc-mir-363-1, ssc-mir-450c, hsa-mir-500b, ssc-mir-194b, ssc-mir-155, ssc-mir-362, bta-mir-3601, ssc-mir-615, ssc-mir-2320, bta-mir-450b, ssc-mir-194a, ssc-mir-196b-2, ssc-mir-363-2, ssc-mir-493, hsa-mir-892c, eca-mir-1388, eca-mir-514b, eca-mir-506a, eca-mir-509b, bta-mir-194b, ssc-mir-1388, ssc-mir-223, ssc-mir-660, bta-mir-194b-2, bta-mir-1949
In pig we found a cluster on chrX of mir-532, mir-188, mir-500, mir-362, mir-500, mir-660, and a mature unknown miRNA picked up by miRDeep. [score:1]
A more difficult case is the mir-532 cluster located on chrX in human (Additional file 1: Figure S14), which contains 8 known miRNAs from miRBase: mir-532, mir-188, mir-500a, mir-362, mir-501, mir-500b, mir-660, mir-502. [score:1]
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[+] score: 2
These findings suggest that the 13 selectively enriched miRNAs we observe in our A33-Exos (miR-3677-3p, -3613-3p*, 652-3p, -3664-3p, -10a-3p*,-3200-3p, -140-3p, 106b-3p*, -15b-3p*, -203-3p, -1307-5p*, -93-3p*, and miR-532-5p) that have not been previously reported in any CRC miRNA studies, to our knowledge, warrant examination as putative candidates for the development of potential CRC markers. [score:2]
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[+] score: 2
[27]↓quail myoblasts diff, ↓ C2C12 diff [29] ↓ C2C12 diff [28] ↓ muscle development [32]40miR-320↓(this study)↑ pMyo diff [33]  41 miR-324-3p (n)↑↑(this study)   42 miR-324-5p (n)↑(this study)   43 miR-331 (n)↑(this study)-  44miR-339↓(this study)↑ C2C12 diff [33] ↑ pMyo diff [33]  45miR-361↑(this study)↑ pMyo diff [33]  46 miR-362↑↑(this study)↑ C2C12 diff [28]  47 miR-374 (n)↑(this study)   48 miR-432 (n)↑(this study)   49 miR-451 (n)↓↓↓(this study)   50 miR-452 (n)↓↓(this study)   51 miR-500↑↑(this study)↑ C2C12 diff [28, 33] ↑ pMyo diff [33]  52 miR-501↑↑↑(this study)↑ C2C12 diff [28, 33]  53 miR-502 (n)↑↑(this study)-  54 miR-503↑(this study)↑ C2C12 diff [19, 28, 33] ↑ pMyo diff [33]  55 miR-532↑↑(this study)↑ C2C12 diff [28, 33] ↑ pMyo diff [33]  56↓↓ pMyo diff. [score:2]
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[+] score: 2
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-20a, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-33a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-93, hsa-mir-96, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-197, hsa-mir-199a-1, hsa-mir-208a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-204, hsa-mir-210, hsa-mir-181a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-mir-224, hsa-mir-200b, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-15b, hsa-mir-27b, hsa-mir-30b, hsa-mir-122, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-138-2, hsa-mir-140, hsa-mir-141, hsa-mir-142, hsa-mir-143, hsa-mir-145, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-126, hsa-mir-138-1, hsa-mir-146a, hsa-mir-193a, hsa-mir-194-1, hsa-mir-195, hsa-mir-206, hsa-mir-320a, hsa-mir-200c, hsa-mir-1-1, hsa-mir-155, hsa-mir-181b-2, hsa-mir-194-2, hsa-mir-106b, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-34b, hsa-mir-34c, hsa-mir-130b, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-363, hsa-mir-365a, hsa-mir-365b, hsa-mir-369, hsa-mir-370, hsa-mir-371a, hsa-mir-375, hsa-mir-378a, hsa-mir-133b, hsa-mir-423, hsa-mir-448, hsa-mir-429, hsa-mir-486-1, hsa-mir-146b, hsa-mir-181d, hsa-mir-520c, hsa-mir-499a, hsa-mir-509-1, hsa-mir-33b, hsa-mir-637, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, hsa-mir-509-2, hsa-mir-208b, hsa-mir-509-3, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, hsa-mir-378b, hsa-mir-320e, hsa-mir-378c, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-371b, hsa-mir-499b, hsa-mir-378j, hsa-mir-486-2
Insulin resistance, obesity, metabolic syndrome, type 2 diabetes, and an adverse lipid profile[207, 208] ↑miR-122 and miR-199a Children obesity[161] ↓miR-375 T1D onset[209] Ortega et al. have reported that morbidly obese patients exhibit a marked increase of circulating miR-140-5p, miR-142-3p, and miR-222 and a decrease of miR-532-5p, miR-125b, miR-130b, miR-221, miR-15a, miR-423-5p, and miR-520c-3p. [score:1]
Insulin resistance, obesity, metabolic syndrome, type 2 diabetes, and an adverse lipid profile[207, 208] ↑miR-122 and miR-199a Children obesity[161] ↓miR-375 T1D onset[209] Ortega et al. have reported that morbidly obese patients exhibit a marked increase of circulating miR-140-5p, miR-142-3p, and miR-222 and a decrease of miR-532-5p, miR-125b, miR-130b, miR-221, miR-15a, miR-423-5p, and miR-520c-3p. [score:1]
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Another study highlighted two miRNAs (again miR-140-5p as well as miR-532-5p) linked to type 2 diabetes that change with insulin sensitization [41]. [score:1]
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79 ** hsa-mir-365 7.13 *** 77.53 *** hsa-mir-429 54.63 *** 85.25 *** hsa-mir-454 33.25 *** 87.31 - hsa-mir-455-3p 42.76 *** 96.4 - hsa-mir-484 4.83 *** 78.73 - hsa-mir-485-3p 4.75 *** 71.49 *** hsa-mir-501-3p 69.25 *** 91.25 *** hsa-mir-512-5p 21.37 *** 72.89 *** hsa-mir-532-3p 9.5 *** 85.93 *** hsa-mir-541 69.87 *** 97.77 - hsa-mir-600 35.63 *** 93.48 - hsa-mir-625* 28.5 *** 72.89 *** Hits of functional screen Relative percentage of myotubes 1, % of control p value, Mann Whitney test Relative cell count 2, % of control p value, Mann Whitney test hsa-mir-636 2.37 *** 81.98 *** hsa-mir-663 21.38 *** 84.73 *** hsa-mir-664 7.13 *** 82.85 *** hsa-mir-766 45.13 *** 73. [score:1]
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Overall, the 40 combinations consisted of 15 different miRNAs (miR-20b, miR-24, miR-28-3p, miR-132-3p, miR-140-3p, miR-146b-5p, miR-155, miR-191, miR-193a-5p, miR-328, miR-331, miR-381, miR-532, miR-628-5p, and miR-660) that were used for further analysis. [score:1]
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Only 9 of the 11 miRNAs isolated could be analysed using the mimiRNA software; and amongst them 6 out of 9 showed a negative correlation, as expected, with the exception of miR-214, miR-24a and miR-532-3p. [score:1]
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However, similarly to some studies already reporting gender effects on some miRNAs for e. g. human brain tissue [44], we found gender specific differences for miR-106a, miR-17 and miR-320 in set A and miR-19a, miR-221, miR-532, miR-95 in set B. Therefore, we decided to control the results towards age and sex by considering these variables as covariates in the confirmatory MANCOVAs. [score:1]
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Each clusters had at least 2 genes, with miR-532∼miR-188 cluster in chromosome X having 2 genes, and mir-363∼106a cluster having 6 genes. [score:1]
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Some miRNAs were related to signal transduction (miR-532-3p and miR-236) and growth factor receptor (miR-1942 and miR-71a). [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-32, hsa-mir-33a, hsa-mir-96, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-16-2, hsa-mir-192, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-10a, hsa-mir-10b, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-182, hsa-mir-183, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-204, hsa-mir-211, hsa-mir-212, hsa-mir-181a-1, hsa-mir-214, hsa-mir-217, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-27b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-137, hsa-mir-138-2, hsa-mir-145, hsa-mir-152, hsa-mir-153-1, hsa-mir-153-2, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-126, hsa-mir-127, hsa-mir-136, hsa-mir-138-1, hsa-mir-146a, hsa-mir-150, hsa-mir-185, hsa-mir-193a, hsa-mir-194-1, hsa-mir-320a, hsa-mir-155, hsa-mir-181b-2, hsa-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-34c, hsa-mir-26a-2, hsa-mir-302b, hsa-mir-369, hsa-mir-375, hsa-mir-378a, hsa-mir-328, hsa-mir-335, hsa-mir-133b, hsa-mir-409, hsa-mir-484, hsa-mir-485, hsa-mir-486-1, hsa-mir-490, hsa-mir-495, hsa-mir-193b, hsa-mir-497, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-506, hsa-mir-509-1, hsa-mir-92b, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-33b, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-1224, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, hsa-mir-802, hsa-mir-509-2, hsa-mir-509-3, hsa-mir-548e, hsa-mir-548j, hsa-mir-548k, hsa-mir-548l, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, hsa-mir-548q, hsa-mir-548s, hsa-mir-378b, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-548w, hsa-mir-320e, hsa-mir-548x, hsa-mir-378c, hsa-mir-4262, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-378f, hsa-mir-378g, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-378h, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-378i, hsa-mir-548am, hsa-mir-548an, hsa-mir-203b, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-378j, hsa-mir-548ay, hsa-mir-548az, hsa-mir-486-2, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-548bc
However, exposure of male mice to stress or paternal stress increases several sperm microRNAs such as, miR-29c, miR-30a, miR-30c, miR-32, miR-193, miR-204, miR-375, miR-532-3p and miR-698 [159]. [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-33a, hsa-mir-98, hsa-mir-29b-1, hsa-mir-29b-2, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-29b-1, mmu-mir-30a, mmu-mir-30b, mmu-mir-126a, mmu-mir-133a-1, mmu-mir-135a-1, mmu-mir-141, mmu-mir-194-1, mmu-mir-200b, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-203a, hsa-mir-211, hsa-mir-218-1, hsa-mir-218-2, hsa-mir-200b, mmu-mir-300, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-135a-1, hsa-mir-135a-2, hsa-mir-141, hsa-mir-194-1, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-21a, mmu-mir-26b, mmu-mir-29a, mmu-mir-29c, mmu-mir-27a, mmu-mir-98, mmu-mir-326, rno-mir-326, rno-let-7d, rno-mir-343, rno-mir-135b, mmu-mir-135b, hsa-mir-200c, mmu-mir-200c, mmu-mir-218-1, mmu-mir-218-2, mmu-mir-33, mmu-mir-211, mmu-mir-29b-2, mmu-mir-135a-2, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-326, hsa-mir-135b, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-21, rno-mir-26b, rno-mir-27b, rno-mir-27a, rno-mir-29b-2, rno-mir-29a, rno-mir-29b-1, rno-mir-29c-1, rno-mir-30c-1, rno-mir-30e, rno-mir-30b, rno-mir-30d, rno-mir-30a, rno-mir-30c-2, rno-mir-33, rno-mir-98, rno-mir-126a, rno-mir-133a, rno-mir-135a, rno-mir-141, rno-mir-194-1, rno-mir-194-2, rno-mir-200c, rno-mir-200a, rno-mir-200b, rno-mir-203a, rno-mir-211, rno-mir-218a-2, rno-mir-218a-1, rno-mir-300, hsa-mir-429, mmu-mir-429, rno-mir-429, hsa-mir-485, hsa-mir-511, mmu-mir-532, rno-mir-133b, mmu-mir-485, rno-mir-485, hsa-mir-33b, mmu-mir-702, mmu-mir-343, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, hsa-mir-300, mmu-mir-511, rno-mir-466b-1, rno-mir-466b-2, rno-mir-532, rno-mir-511, mmu-mir-466b-4, mmu-mir-466b-5, mmu-mir-466b-6, mmu-mir-466b-7, mmu-mir-466b-8, hsa-mir-3120, rno-mir-203b, rno-mir-3557, rno-mir-218b, rno-mir-3569, rno-mir-133c, rno-mir-702, rno-mir-3120, hsa-mir-203b, mmu-mir-344i, rno-mir-344i, rno-mir-6316, mmu-mir-133c, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-mir-30f, mmu-let-7k, mmu-mir-3569, rno-let-7g, rno-mir-29c-2, rno-mir-29b-3, rno-mir-466b-3, rno-mir-466b-4, mmu-mir-203b
Type of site Context+ Context Structure Energy Is experimental validated rno-miR-326-5p MIMAT0017028 3 8mer 7mer-m8 imperfect −0.442 −0.242 431 −65.97 TRUE rno-miR-485-5p MIMAT0003203 2 7mer-m8 −0.343 −0.372 290 −34.96 TRUE rno-miR-300-5p MIMAT0004743 1 8mer −0.338 −0.421 156 −15.16 TRUE rno-miR-702-5p MIMAT0017884 1 8mer −0.317 −0.274 142 −13.86 TRUE rno-miR-203b-3p MIMAT0017800 2 7mer-m8 −0.298 −0.421 295 −29.93 TRUE rno-miR-33-3p MIMAT0017104 2 8mer 7mer-m8 −0.297 −0.813 305 −22.7 TRUE rno-miR-466b-3p MIMAT0017285 1 8mer −0.295 −0.47 159 −15.26 TRUE rno-miR-532-5p MIMAT0005322 1 7mer-m8 −0.268 −0.302 151 −10.71 TRUE rno-miR-511-5p MIMAT0012829 1 7mer-m8 −0.268 −0.302 152 −10.37 TRUE rno-miR-343 MIMAT0000591 1 7mer-m8 −0.262 −0.24 140 −13.75 TRUE rno-miR-203a-3p MIMAT0000876 1 8mer −0.245 −0. [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-31, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-181a-2, hsa-mir-205, hsa-mir-181a-1, hsa-mir-214, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-146a, hsa-mir-184, hsa-mir-186, hsa-mir-193a, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-219a-2, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-365a, hsa-mir-365b, hsa-mir-374a, hsa-mir-148b, hsa-mir-423, hsa-mir-486-1, hsa-mir-499a, hsa-mir-590, bta-mir-26a-2, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-221, bta-mir-222, bta-mir-27a, bta-mir-499, bta-mir-125b-1, bta-mir-181a-2, bta-mir-205, bta-mir-27b, bta-mir-30b, bta-mir-31, bta-mir-193a, bta-let-7d, bta-mir-148b, bta-mir-186, bta-mir-191, bta-mir-192, bta-mir-200a, bta-mir-214, bta-mir-22, bta-mir-23a, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-mir-532, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-365-1, bta-mir-374a, bta-mir-99b, hsa-mir-374b, hsa-mir-664a, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-1915, bta-mir-146a, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-194-2, bta-mir-219-1, bta-mir-223, bta-mir-26a-1, bta-mir-365-2, bta-mir-374b, bta-mir-486, bta-mir-763, bta-mir-9-1, bta-mir-9-2, bta-mir-181a-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2339, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-664a, bta-mir-2284e, bta-mir-1388, bta-mir-194-1, bta-mir-193a-2, bta-mir-2284w, bta-mir-2284x, bta-mir-148c, hsa-mir-374c, hsa-mir-219b, hsa-mir-499b, hsa-mir-664b, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2284z-2, hsa-mir-486-2, hsa-mir-6516, bta-mir-2284ab, bta-mir-664b, bta-mir-6516, bta-mir-219-2, bta-mir-2284ac, bta-mir-219b, bta-mir-374c, bta-mir-148d
Moreover, a false precursor of bta-miR-532 predicted by miRDeep2 (Additional file 1: Table S1.2) contained a star miRNA of 17 nucleotides in length, indicating that a precursor generating a short miRNA may have a high false positive rate. [score:1]
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