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24 publications mentioning hsa-mir-519e

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-519e. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 56
miR-519, a microRNA that suppresses tumorigenesis and lowers expression of RNA -binding protein HuR, was upregulated in senescent cells. [score:8]
In conjunction with the finding that miR-519 reduced tumorigenesis in a xenograft mo del [32], we propose that the coordinated action of senescence -upregulated microRNAs can suppress tumor growth by reducing the levels of oncogenes or tumor promoters. [score:6]
Among the microRNAs showing increasing abundance with senescence, miR-519 was of particular interest because it was shown to inhibit translation of HuR and to diminish tumor growth [31, 32]. [score:5]
miR-519 was recently found to inhibit translation of the RNA -binding protein HuR through its interaction with the HuR coding region [31]. [score:5]
As indicated above, miR-519 was found to suppress tumor growth [32]. [score:3]
Overexpression of miR-519 induced senescence in WI-38 and HeLa cells. [score:3]
As shown here for miR-519, we postulate that these changes help to meet the needs of senescent cells in eliciting tumor suppression and growth arrest. [score:3]
Since HuR potently enhances the expression of cancer-promoting proteins, and reducing HuR levels promotes HDF senescence [11, 38], we propose that miR-519 represses tumor growth at least in part, by lowering HuR and thereby promoting senescence (Figs. 4 and 5). [score:3]
Additionally, miR-519 could further repress tumor growth by lowering the expression of other genes, such as ABCG2 or HIF-1α [46, 47]. [score:3]
In a separate study, miR-519 suppressed the growth of tumor xenografts in an HuR -dependent manner [32]. [score:3]
Accordingly, we postulate that one of the mechanisms by which miR-519 suppress tumor growth is by inducing senescence, and further propose that miR-519 triggers senescence -at least in part- by reducing HuR levels. [score:3]
Given that HuR promotes cell proliferation and decreases senescence [33, 34], we hypothesized that the elevated miR-519 in senescent cells (Figure 4A) might lower HuR expression in WI-38 HDFs, and hence promote senescence. [score:3]
We were particularly interested in the miR-519 family. [score:1]
Together, these data indicate that miR-519 reduced HeLa cell proliferation and promoted HeLa cell senescence. [score:1]
miR-519 -induced senescence in HDFs. [score:1]
miR-519 -induced senescence in HeLa cells. [score:1]
We previously reported that miR-519 represses the production of HuR, an RNA -binding protein which is highly abundant in cancer cells and is low in untransformed cells [11, 38]. [score:1]
Moreover, while HuR levels are high in tumors and low in normal tissues, miR-519 levels are high in normal tissues and low in cancer tissues [32]. [score:1]
Influence of miR-519 on the senescent phenotype of HeLa cells. [score:1]
Influence of miR-519 on WI-38 senescence. [score:1]
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2
[+] score: 33
The numerical in brackets shows the ranking of each pathway Table 3 Common validated target genes shared between the C19MC-AAGUGC-miRNAs and the miR-302/-372 families AAGUGC-miRNASeed position [a] Target transcript miR-302/-372 C19MC miR-302c miR-520e I NIK[10, 15] miR-373 miR-520c I MT1-MMP, mTOR, SIRT1[14, 21] miR-372, -373 miR-520c, -520e I RelA[12] miR-302b, -372, -373 miR-520c, -520e I TGFβR2[9, 12] miR-520b, -520e I CD46[16] miR-302c miR-520c I MICA, MICB, ULBP2[17] miR-519a I RBL2[13] miR-512 IIa miR-519d, -520g IIb SMAD7[19, 20] miR-520g, -520h IIb DAPK2[18, 22] miR-302d, -372 miR-520b, -519b-3p, -520a-3p I CDKN1A[5, 6] miR-519e IIa miR-519d, -520h IIb [a]Group I seed position is the canonical nts 2-7; IIa is nts 1-6 and IIb is other non-canonical position, as defined in Fig.   2a The 2058 putative target genes were further subjected to GO analysis and KEGG pathway annotation (Fig.   3b-d). [score:7]
The numerical in brackets shows the ranking of each pathway Table 3 Common validated target genes shared between the C19MC-AAGUGC-miRNAs and the miR-302/-372 families AAGUGC-miRNASeed position [a] Target transcript miR-302/-372 C19MC miR-302c miR-520e I NIK[10, 15] miR-373 miR-520c I MT1-MMP, mTOR, SIRT1[14, 21] miR-372, -373 miR-520c, -520e I RelA[12] miR-302b, -372, -373 miR-520c, -520e I TGFβR2[9, 12] miR-520b, -520e I CD46[16] miR-302c miR-520c I MICA, MICB, ULBP2[17] miR-519a I RBL2[13] miR-512 IIa miR-519d, -520g IIb SMAD7[19, 20] miR-520g, -520h IIb DAPK2[18, 22] miR-302d, -372 miR-520b, -519b-3p, -520a-3p I CDKN1A[5, 6] miR-519e IIa miR-519d, -520h IIb [a]Group I seed position is the canonical nts 2-7; IIa is nts 1-6 and IIb is other non-canonical position, as defined in Fig.   2a The 2058 putative target genes were further subjected to GO analysis and KEGG pathway annotation (Fig.   3b-d). [score:7]
However, construction of a Venn diagram showed that only 262 putative target genes are common between the miR-519 and miR-520 subfamilies in group I, indicating that the miR-519 and -520 subfamilies target different sets of genes. [score:5]
Genes targeted by either or both the miR-519 or -520 subfamilies are shown in different color boxes. [score:3]
Furthermore, one of the characteristics of target prediction, the sequence context surrounding the seed binding site of the target transcript [1], between the miR-519 and -520 subfamilies are also dissimilar (Fig.   2a). [score:3]
The group I miR-519 subfamily also shares 262 putative target genes with the miR-302/-372 families, far fewer than the miR-520 subfamily (Fig.   3a, red box). [score:3]
In this study, the putative target sets of the miR-519 and -520 subfamilies are overlapping gene sets predicted by two different prediction algorithms. [score:3]
The group I miRNAs are composed of the miR-519 and -520 subfamilies. [score:1]
Furthermore, it is noted that the AAGUGC seed position at 5’ end is variable among the C19MC-AAGUGC-miRNAs: subgroup I miRNAs, which includes eight miR-519 and -520 subfamilies, have the seed sequence located at the canonical and optimal 5’-nucleotide positions (nts) 2-7, as in the miR-302/-372 families; the seed sequence of the four subgroup IIa miRNAs is at location nts 1-6, and that of the remaining subgroup IIb miRNAs is at nts 3-8 and 4-9 (Fig.   2a). [score:1]
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[+] score: 17
The mechanism underlying selective upregulation of the ORAI1 subunit is not known, but the post-transcriptional expression of the ORAI1 gene has been shown to be regulated by miR-519 using TargetScan/complementarity studies in HELA cells (Ab delmohsen et al., 2012). [score:9]
Whether similar regulation by MIR-519 occurs in hVF-HF is not known, but in our studies we were unable to detect MIR-519 expression in fibroblasts from non-failing or failing hearts, which is suggestive that other possible regulatory mechanisms are involved (Sauc et al., 2015). [score:5]
Growth inhibition by miR-519 via multiple p21-inducing pathways. [score:3]
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[+] score: 17
Other miRNAs from this paper: hsa-mir-34a, hsa-mir-381
For example, hsa-miR-34a was up-regulated (t-test p = 0.0001), while its gene target, FUT8 (encoding fucosyltransferase 8) was down-regulated in transplant patients; and hsa-miR-519e was up-regulated (t-test p = 0.003), consistent with down-regulation of its potential targets, ATR (encoding ataxia telangiectasia and Rad3 related) and PYHIN1 (encoding pyrin and HIN domain family, member 1). [score:17]
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[+] score: 10
Mir-519 and -519E were also upregulated in cKit+ ILCs; these microRNAs have been shown to inhibit cell growth and thus promote cell survival [25]whereas mir-423 promotes cell growth by regulating the G(1)/S transition in the cell cycle in hepatocellular carcinoma cell lines [26]. [score:7]
Mirs 365-2 and 365-1 (49.6 and 20, respectively), as well as mir-1324 (35.1) and mir-519e [19] were very highly expressed in cKit+ ILCs and not by control cells (Figure 6C). [score:3]
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[+] score: 9
We then applied real-time qRT-PCR to validate the expression of two up-regulated miRNAs (hsa-miR-27a-3p and hsa-miR-146a-5p) and two down-regulated miRNAs (hsa-miR-20b-3p and hsa-miR-519e-5p) from the HLEC and colon cancer cell co-culture system. [score:9]
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[+] score: 6
detected the expression profile of miRNAs from blood samples of influenza A H1N1 virus-infected patients and then exhibited that the expression levels of 193 miRNA molecules were altered in all influenza patients, of which 16 highly dysregulated miRNAs (miR-1260, miR-1285, miR-18a, miR-185*, miR-299-5p, miR-26a, miR-30a, miR-335*, miR-34b, miR-519e, miR-576-3p, miR-628-3p, miR-664, miR-665, miR-765 and miR-767-5p) were able to provide a clear distinction between infected and healthy individuals [39]. [score:6]
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[+] score: 6
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-29a, hsa-mir-30a, hsa-mir-98, hsa-mir-101-1, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-106a, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-10a, hsa-mir-10b, hsa-mir-34a, hsa-mir-210, hsa-mir-215, hsa-mir-200b, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-30b, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-137, hsa-mir-138-2, hsa-mir-143, hsa-mir-144, hsa-mir-145, hsa-mir-152, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-126, hsa-mir-127, hsa-mir-138-1, hsa-mir-146a, hsa-mir-193a, hsa-mir-194-1, hsa-mir-206, hsa-mir-320a, hsa-mir-200c, hsa-mir-1-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-106b, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-302a, hsa-mir-101-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-369, hsa-mir-371a, hsa-mir-340, hsa-mir-335, hsa-mir-133b, hsa-mir-146b, hsa-mir-519c, hsa-mir-519b, hsa-mir-519d, hsa-mir-519a-1, hsa-mir-519a-2, hsa-mir-499a, hsa-mir-504, hsa-mir-421, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-190b, hsa-mir-301b, hsa-mir-302e, hsa-mir-302f, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, hsa-mir-320e, hsa-mir-371b, hsa-mir-499b
Small RNA regulation of some of the splicing control proteins has previously been reported; for example, the ELAV transcript, which encodes the HuR protein, a major modulator of mRNA stability and translation in addition to mRNA splicing [58], is subject to regulation by miRNAs miR-519, miR-16 and miR-125a in a variety of cell types including cervical, ovarian and colon cancer cell lines [59, 60, 61]. [score:5]
Ab delmohsen K. Srikantan S. Kuwano Y. Gorospe M. miR-519 reduces cell proliferation by lowering RNA -binding protein HuR levels Proc. [score:1]
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[+] score: 5
Finally, when miRNA expression pattern was linked to the cause of HCC, we found that the expression level of miR-181d, miR-542-3p, and miR-519e in HCC derived from CH was significantly higher than in HCC from liver cirrhosis (LC). [score:5]
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[+] score: 4
Recently identified miRNAs, such as miR-9, miR-34a, miR-16, miR-125a, miR-29a, miR-200c, and miR-519, play crucial roles in regulating HuR expression through interaction of miRNAs with specific sites in the 3′UTR and 5′UTR of the HuR mRNA. [score:4]
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[+] score: 4
E) Quantification of SMYD2 protein level in HEK293T transfected with either pre-miR-519-3p or random miRNA sequences. [score:1]
D) Quantification of Smyd2 mRNA level in HEK293T transfected a plasmid containing either pre-miR-519-3p or random miRNA sequences. [score:1]
F) Western-blot of HEK293T protein extract, transfected either with pre-miR-519-3p or random miRNA sequences. [score:1]
Validation of Smyd2 mRNA interaction with miR-519. [score:1]
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12
[+] score: 4
miR-212 and miR-519e were also upregulated in the impaired monocyte but did not reach statistical significance (P = 0.09). [score:4]
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13
[+] score: 4
MiR-519 represses the production of HuR, an RNA -binding protein very abundantly found in tumor cells and less expressed in untransformed cells, while the overexpression of HuR delays the senescent phenotype (83). [score:4]
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[+] score: 3
Other miRNAs from this paper: hsa-mir-511, hsa-mir-515-1, hsa-mir-515-2, mmu-mir-511, hsa-mir-4711
Of the Renilla luciferase reporter construct (phRL-TK rs2734647C, or rs2734647T, respectively), 1 μg (per well), plus 1 μg (per well) of the reference construct pCMV-LacZ (Clontech, Mountain View, CA, USA) were co -transfected in the presence of 10 pg of mirVana miRNA mimic hsa-miR-4711-3p, hsa-miR-511, hsa-miR-515-3p, hsa-miR-519e-3p, negative control #2 (all Life Technologies, Darmstadt, Germany), or no miRNA, respectively. [score:1]
While miR-4711-3p and miR-511 are predicted to show preferential binding in case of the presence of the T-allele, miR-515-3p has a strong negative ΔΔG only in case of the C-allele, and miR-519e lacks a strong allele preference. [score:1]
Co-transfection with miR-515-3p or miR-519e did not lead to luciferase activity reduction for either rs2734647 T or C (Fig  6B). [score:1]
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[+] score: 3
For example, miR-144, miR-937, miR-376, miR-519, and miR-548A-3P are shown to regulate a number of mRNAs, and HCK, NFKBIE, IL6, SHMT2, and MCM4 are regulated by several miRNAs. [score:3]
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[+] score: 3
In addition to the known miRNAs, the revealed other differentially enriched miRNAs, such as miR-887/miR-24-2 in endothelial cells and miR-519e in hPSCs (Fig.   3d and Additional file 5: Figure S3D). [score:1]
However, for some less abundant miRNAs, loss of peaks was identified in some samples with either 1 cell or 10 cells (Additional file 6: Figure S4E, miR-24-2 and miR-519e). [score:1]
qPCR (e) and representative denaturing PAGE (12%) of the amplified products (f) of miR-302c, miR-367, miR-498, miR-515, miR-519e, miR-19b, miR-92a, miR-24-2, miR-126-3p, miR-126-5p, and miR-887. [score:1]
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17
[+] score: 3
Our analysis shows a possible involvement of several members of the miR-515 family (such as miR-515-5p, miR-519 and miR-520a-3p/b/c-3p/d-3p/e/g/h), located at 19q13.4, in POF, since they are predicted to target FMR1 and FOXL2, two genes which are associated to POF [47], [48]. [score:3]
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[+] score: 1
Seed sequence examination indicated no similarities to the known seed sequences of pluripotency-specific miRNAs such as AAGUGC in miR-302b-3p, miR-373, miR-520e, miR-519c-3p, miR-520a-3p, and miR-520b; AGUGCC in miR-515-3p and miR-519e; and AAGUG in miR-519d. [score:1]
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[+] score: 1
For example, rs2999200 was found to be significantly associated with miR-198, miR-509-3-5p and miR-519e* (p = 8.8x10 [-13], p = 6.9x10 [-11] and p = 4.3x10 [-13], respectively). [score:1]
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[+] score: 1
Of these, miR-302a and miR-302d are members of the miR-302 family and miR-519e, -519b, and -517a belong to the miR-515 family. [score:1]
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[+] score: 1
2010.00683. x 20649821 8. Ab delmohsen K. Srikantan S. Kuwano Y. Gorospe M. miR-519 reduces cell proliferation by lowering RNA -binding protein HuR levels Proc. [score:1]
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[+] score: 1
Besides these 9 miRNAs, we also identified 12 more miRNAs in this cluster; they were miR-515-5p, miR-517a, miR-517b, miR-517c, miR-519e, miR-520b, miR-520d, miR-520f, miR-520h, miR-521, miR-525-3p, and miR-526b*. [score:1]
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[+] score: 1
The miR-659-3p cluster also included miR-219-3p and miR-519-5p (Fig.   1b), which had less significant or no association with PFS (p = 0.036 and p = 0.12, respectively). [score:1]
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[+] score: 1
039  hsa-mir-519e-3p 3.768. [score:1]
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