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11 publications mentioning ssc-mir-9-2

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-9-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 115
In abdominal fat the expression of both mir-9 and mir-124a was found to be more highly expressed in obese males (mir-124a FC: 119.8; p-value = 0.0108, mir-9 FC: 7.8; p-value = 0.07) and both were significantly more highly expressed in the liver of obese males (mir-124a FC: 12.3; p-value = 0.013, mir-9 FC: 1.6; p. value = 0.036) while no difference in expression could be detected in the females. [score:9]
Mir-9 over -expression in β-cells induces exocytosis and thereby insulin release by targeting the transcription factor Onecut, which negatively regulates granuphilin. [score:5]
Both the 3’ and 5’ mature miRNAs of mir-9 were significantly differentially expressed in the sequencing analysis, but only mir-9-5p was tested in qPCR due to low expression of mir-9-3p. [score:5]
In Fig 2 the expression of mir-9 and mir-124a in lean and obese subcutaneous adipose tissue is illustrated in a Column scatter plot as the relative mean of log2 fold change were the lowest expressed sample of each miRNA assay is set to 1. Mir-9 and mir-124a are both significantly up regulated in obese animals with even higher fold changes than found in the sequencing study. [score:5]
Two of the 6 miRNAs found to be differentially expressed in the sequencing study, mir-9 and mir-124a, were significantly differentially expressed between the two groups (Table 6, Fig 2). [score:5]
qPCR validation of these results in general confirms the expression pattern, however, statistical significant differential expression was only observed for two of the six miRNAs, mir-9 and mir-124a (Table 6, Fig 2). [score:5]
A selection of target genes for mir-9 and mir-124 (list in S7 Dataset) were tested by qPCR but none of them were significantly suppressed in the obese group (S8 Dataset). [score:5]
Mir-9 expression has also been linked to the obesity related diseases diabetes and inflammation. [score:4]
The overexpression of mir-9 and mir-124 in the adipose tissue of obese pigs may also contribute to the lipid accumulation in the adipocytes. [score:3]
Among these the expression profiles of mir-124a-3p and mir-9-3p overlapped with the results for the analysis of the combined dataset. [score:3]
Student’s t-test of mir-9 and mir-124a qPCR Expression in Liver and Abdominal Adipose Tissue. [score:3]
Mir-9 and mir-124a were both significantly differentially expressed in the liver of obese males, while there was no significant difference in the females. [score:3]
In abdominal adipose tissue both mir-9 and mir-124a was expressed at a higher level in the obese male pigs, (p-value = 0.07 and 0.01 respectively). [score:3]
Differential expression in the sequencing data from the lean and obese pigs was calculated using the DESeq2 package in R and revealed six significantly differentially expressed miRNAs between the two groups: mir-9-5p, mir-124a-3p, mir-9-3p, mir-199a-5p, mir-489-3p and mir-34c-3p. [score:3]
Expression of mir-9 and mir-124a in Abdominal Adipose Tissue and Liver. [score:3]
When the qPCR results were analyzed for male pigs only, mir-99a was differentially expressed in addition to mir-124a and mir-9 (Table 6). [score:3]
Both mature arms of mir-9 were significantly differentially expressed in the sequencing data, but only the mir-9-5p was tested in qPCR. [score:3]
qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). [score:3]
0131650.g002 Fig 2 qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). [score:3]
Additionally, only mir-124 is significantly up regulated in abdominal adipose tissue of male obese pigs and both mir-9 and mir-124a are up regulated in the liver of obese male pigs. [score:3]
0131650.g003 Fig 3 qPCR expression of mir-9 and mir-124a in Abdominal Adipose Tissue (Abd AT) (A,B) and Liver (C,D). [score:3]
Fold changes and p-values for qPCR in subcutaneous adipose tissue can be found in Table 6. To further study the expression of mir-9 and mir-124a, the two miRNAs with the largest fold changes between the lean and obese group in the subcutaneous adipose tissue, qPCR was also performed on cDNA from abdominal adipose tissue, liver and muscle from the same animals as in the study of subcutaneous adipose tissue. [score:3]
Expression of mir-9 and mir-124a in Subcutaneous Adipose Tissue. [score:3]
Inactivated lipid carrying HSCs have higher mir-9 and mir-124 expression than activated HSCs that carry no lipids [63]. [score:3]
MiRTarbase Verified mir-9 and mir-124 Target Genes. [score:3]
qPCR expression of mir-9 (A) and mir-124a (B) in Subcutaneous Adipose Tissue (SC AT). [score:3]
In the female pigs mir-9, mir-124a, mir-103, mir-10b and mir-99a were differentially expressed (Table 6). [score:3]
The data is presented as column scatter plots in Fig 3. Mir-9 and mir-124a were expressed in all three tissues. [score:3]
Analysis of mir-9 and mir-124a target genes has not previously been performed in adipose tissue. [score:3]
QPCR studies confirm some of these differences, in particular for mir-9 and mir-124a which are significantly differentially expressed with large fold changes. [score:3]
Mir-9 expression is also induced in monocytes and neutrophils upon activation of the immune receptors TLR4, TLR2 and TLR7/8 and by the pro-inflammatory cytokines TNF-a and IL-1B. [score:2]
Mir-9 and mir-124a are significantly up regulated in subcutaneous adipose tissue of obese pigs, independently of gender. [score:2]
An example is that mir-9 and mir-124 are slightly up regulated in the blood of diabetic patients compared to non-diabetic controls [52]. [score:1]
This is, to our knowledge, the first study of subcutaneous adipose tissue of lean versus obese subjects where mir-9 and mir-124a have been shown to be significantly up regulated in the obese subjects with large fold changes compared to the lean subjects. [score:1]
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[+] score: 44
In humans, CXCR4 is a verified target gene of miR-9 [64] and there is extensive support for a human target site in Table 1, but no support for a porcine target site. [score:7]
MiR-9-5p, miR-148a and miR-125a also have target sites in SCD, which is upregulated in the adipose tissue of the obese minipigs. [score:6]
In contrast, MiR-9-5p is upregulated in serum of human diabetic patients and, in another study, upregulated in porcine adipose tissue from a mixed breed population [10, 40]. [score:6]
LEP has target sites for three miRNAs: MiR-30a, miR-148a and miR-9-5p which were all downregulated in obese adipose tissue and muscle. [score:6]
LEP was the gene containing the most miRNA target sites, i. e. is targeted by miR-148a-3p, miR-125a-5p, miR-30a, miR-9-5p and miR-17-5p. [score:5]
However, miR-9 was highly downregulated (FC 7.6; p value 0.046). [score:4]
MiR-9 (FC -8.5; p value 0.02) was the most downregulated miRNA. [score:4]
CXCL14 has a target site for miR-9-5p. [score:3]
SCD is also targeted by many of the same miRNAs, namely miR-148a-3p, miR-125a-5 and miR-9-5p. [score:3]
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[+] score: 18
It has been established that miRNAs can target specific genes [23]; in the present study, let-7c, let-7 g, miR-18a, miR-196b, and miR-9 targeted MAP3K7, and miR-196b and miR-19b targeted dipeptidyl-peptidase 8 (DPP8), suggesting that cellular miRNAs play a key role in regulating gene expression in response to PPV infection. [score:10]
Among them, let-7 g, miR-17-5p, miR-17-3p, miR-20a, miR-181a, miR-16, miR-146b, miR-10b, and miR-155-5p were upregulated; let-7c, miR-122, miR-18a, miR-19a, miR-19b, miR-196b, miR-21, and miR-9 were downregulated. [score:7]
Many immune-related miRNAs have been identified in innate and adaptive immune systems, including the miR-17—92 cluster, miR-221, miR-10, miR-196b, miR-126, miR-155, miR-150; miR-181a, miR-326, miR-142-3p, miR-424, miR-21, miR-106a, miR-223, miR-146; the let-7 family, miR-9, and miR-34 [6]. [score:1]
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[+] score: 11
In both pig breeds, 14 miRNAs were identically up-regulated after sexual maturity (Supplementary Table S14); the relative expression fold-change of miR-145-5p, miR-205, miR-34c, miR-9, miR-9-1 and miR-9-2 at 200 days old were more notable, especially that of miR-34c, which were consistent with the findings of Lian 30 (Fig. 3). [score:6]
We found that miR-143-5p, miR-29c, miR-9, miR-9-1, miR-9-2 and piR_20121 were correlated with the target NUP160, whereas miR-22-3p, miR-29c and piR_16678 were correlated with the target TET3. [score:5]
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[+] score: 11
For example, the significantly differentially expressed miRNAs, miR-9, miR-9–1 and miR-9–2 all target IL12A, but the immune-related gene IL7R was individually regulated by miR-140–3p. [score:6]
This study also found a series of miRNAs that target host-encoded pre-miRNAs; for example, ssc-miR-9, ssc-miR-19a, ssc-miR-142–5p, ssc-miR-134 and ssc-miR-20c-5p all target ssc-mir-21. [score:5]
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[+] score: 7
A total of forty cellular miRNAs were differentially expressed in PRRSV infected macrophages, six of which (miR-30a-3p, miR-132, miR-27b*, miR-29b, miR-146a and miR-9-2) were altered at more than one time point (Figure 1). [score:3]
Of the differentially expressed miRNAs six (miR-30a-3p, miR-132, miR-27b*, miR-29b, miR-146a and miR-9-2) were altered at more than one time point in PRRSV-infected macrophages (Figure 1). [score:3]
Among these six miRNA, miR-30a-3p, miR-132, miR-27b*, miR-29b, miR-146a and miR-9-2, were altered at more than one time point. [score:1]
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[+] score: 7
In our study, prv-miR-1, prv-miR-3 and prv-miR-11 were predicted to target both LORF1 and LORF2, while prv-miR-2 and prv-miR-9 target LORF1, which reveal that there exist a similar self-regulation mechanism of PRV LLT and its miRNAs. [score:6]
Four other pre-miRNAs (prv-miR-1, prv-miR-5, prv-miR-9 and prv-miR-11) and one mature miRNA, prv-miR-1-3′, were detected by northern blot as well (Figure 2F and Table 1). [score:1]
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[+] score: 5
Moreover, we also detected three new miRNAs (new-miR-9, new-miR-64, and new-miR-80) differentially expressed in response to 1.5% CLA treatment, and new-miR-9 was a differential expression miRNA in both of the two adipose tissues (Table S10). [score:5]
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[+] score: 4
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-27a, hsa-mir-30a, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-107, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-30a, mmu-mir-30b, mmu-mir-125b-2, mmu-mir-9-2, mmu-mir-150, mmu-mir-24-1, mmu-mir-204, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-204, hsa-mir-210, hsa-mir-221, hsa-mir-222, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-150, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-21a, mmu-mir-24-2, mmu-mir-27a, mmu-mir-103-1, mmu-mir-103-2, mmu-mir-326, mmu-mir-107, mmu-mir-17, mmu-mir-210, mmu-mir-221, mmu-mir-222, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-125b-1, hsa-mir-30c-1, hsa-mir-30e, hsa-mir-378a, mmu-mir-378a, hsa-mir-326, ssc-mir-125b-2, ssc-mir-24-1, ssc-mir-326, ssc-mir-27a, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-204, ssc-mir-21, ssc-mir-30c-2, ssc-mir-9-1, hsa-mir-378d-2, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-15a, ssc-mir-17, ssc-mir-30b, ssc-mir-210, ssc-mir-221, ssc-mir-30a, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-30d, ssc-mir-30e, ssc-mir-103-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-222, ssc-mir-125b-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-30c-1, ssc-mir-378-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, mmu-mir-378b, ssc-let-7a-2, hsa-mir-378j, mmu-mir-21b, mmu-let-7j, mmu-mir-378c, mmu-mir-21c, mmu-mir-378d, mmu-mir-30f, ssc-let-7d, ssc-let-7f-2, ssc-mir-9-3, ssc-mir-150-1, ssc-mir-150-2, mmu-let-7k, ssc-mir-378b, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
We found 13 adipogenesis-promoting miRNAs (let-7、miR-9、miR-15a、miR-17、miR-21、miR-24、miR-30、miR-103、miR-107、miR-125b、miR-204、miR-210、and miR-378) target 860 lncRNA loci. [score:3]
We analyzed the relationship between the 343 identified lncRNAs with the 13 promoting adipogenesis miRNAs (let-7、miR-9、miR-15a、miR-17、miR-21、miR-24、miR-30、miR-103、miR-107、miR-125b、miR-204、miR-210、and miR-378) and five depressing adipogenesis miRNAs (miR-27, miR-150, miR-221, miR-222, and miR-326). [score:1]
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[+] score: 3
Among the non-profile-shared miRNAs, there were 6 high expressed miRNAs (CN>1,000 counts): miR-99a-5p (11,178 counts), miR-10a-5p (3,570 counts), miR-133a (1,990 counts), miR-218b (1,887 counts), miR-9-3p (1,620 counts) and miR-129a (1,566 counts). [score:3]
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[+] score: 1
In accordance with the miRNA naming conventions adopted by miRBase, these identical miRNAs from different pre-miRNAs were distinguished by the suffix such as ssc-miR-9-1 and ssc-miR-9-2 [11]. [score:1]
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