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12 publications mentioning gga-mir-31

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-31. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 104
As shown in Fig 3, transfection of bantam and miR-31 miRNA mimics resulted in a reduction of luciferase activity compared to transfection with scrambled miRNA mimics, indicating that these miRNAs can down-regulate the expression of the corresponding schistosome mRNA target regions in a heterologous system. [score:7]
miR-1b, miR-61 and miR-281 were highly expressed in males whereas miR-8447, miR-2f, mir-8437, miR-31, bantam, miR-2c, miR-2d, miR-71b, miR-36b and miR-755 were highly expressed in females. [score:5]
qRT-PCR analyses of the expression of miR-31 (A) or bantam miRNA (B) in female schistosomes treated with miRNA inhibitor. [score:5]
We also demonstrated that schistosome smad1 (a target gene of bantam) and Frizz7 (a target gene of miR-31) are predominantly localized in the ovary of S. japonicum, consistent with their localization in S. mansoni [88– 90]. [score:5]
To determine whether these miRNAs could repress mRNA targets in vivo in schistosomes, we introduced bantam and miR-31 antisense miRNAs by electroporation into in vitro cultured adult female schistosomes and determined if their target mRNA levels changed using qRT-PCR. [score:5]
The co-localization of the miRNAs and their target mRNAs, at least for bantam miRNA and miR-31, suggest their coordinated involvement in the regulation of ovarian development. [score:5]
From these data, we conclude that bantam and miR-31 are involved in the regulation of target genes that are instrumental in ovary development and oocyte maturation. [score:5]
These results suggest that bantam and miR-31 and their target mRNAs play important roles in the development of the structure and architecture of the ovary and in oocyte differentiation. [score:4]
S11 Fig (A) Effect of miR-31 suppression on worm mortality in female schistosomes. [score:3]
Quantitation of ovary changes due to miR-31 (C) and bantam miRNA suppression (D). [score:3]
S8 FigZ-Stack of optical sections from S. japonicum ovary treated with a scrambled miR-31 inhibitor. [score:3]
To test whether miR-31 and bantam miRNA suppression would lead to any morphological changes in female schistosomes, 24–28 day-old female worms were cultured in vitro for four days following electroporation of antisense miR-31 or bantam into worms. [score:3]
Suppression of female enriched miRNAs bantam and miR-31 results in morphological alternation of ovaries in female schistosomes. [score:3]
Z-Stack of optical sections from S. japonicum ovary treated with the miR-31 inhibitor. [score:3]
Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Movie showing a Z-stack series of the ovary treated with a scrambled miR-31 inhibitor. [score:3]
Movie showing a Z-stack series of the ovary treated with a miR-31 inhibitor. [score:3]
1005423.g003 Fig 3Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Four female enriched miRNAs (miR-31, bantam, miR-1989 and miR-2c) were shown to be predominantly expressed in the region of ovary (Fig 5A and 5B). [score:3]
We next demonstrated that the target mRNAs of bantam (Smad1) or miR-31 (Frizz7) are also primarily localized in ovary of female schistosomes (S6 Fig and S10 Table). [score:3]
By examining the ratio of the total area of ovary to the area occupied by oocytes, miR-31/bantam inhibitor resulted in a significant reduction of parenchymal cells and oocytes in the ovary of female schistosomes (Fig 6C and 6D). [score:3]
1005423.g006 Fig 6 Effect of miR-31 (A) or bantam (B) suppression on female ovary architecture and morphology. [score:3]
S7 FigZ-Stack of optical sections from S. japonicum ovary treated with the miR-31 inhibitor. [score:3]
Z-Stack of optical sections from S. japonicum ovary treated with a scrambled miR-31 inhibitor. [score:3]
In addition, suppression of female enriched miRNAs such as miR-31 and bantam led to morphological changes in the ovaries of female schistosomes. [score:3]
These findings strongly imply that female enriched miRNAs such as bantam and miR-31 may be key regulators in S. japonicum ovarian development. [score:3]
Furthermore, RNA levels of miR-31 and bantam determined by qRT-PCR were also significantly reduced in target/antisense RNA -treated worms compared to control worms (S12 Fig). [score:2]
miR-31 and bantam are predominantly present in the ovaries of female schistosomes (Fig 5), suggesting that these miRNAs may play an important role in ovary development. [score:2]
We demonstrated that female-enriched miR-31 can regulate the mRNA for Frizz 7 (Accession No: EU370927, a receptor of WNT signaling pathway) and an O-glycosyltransferase (Accession No: FN319623.1) whereas the female-enriched miR-1989 likely interacts with asparagine-rich protein mRNA (Accession No. [score:2]
To further corroborate these results, we selected two of the female enriched miRNAs, bantam and miR-31, and used in situ hybridization to demonstrate that they predominantly localize to the ovary (Fig 5C and S10 Table). [score:1]
Similar results were also observed in the schistosomes treated with antisense miR-31 (Fig 4B). [score:1]
Hela or HEK293T cells were transfected with these recombinant plasmids, control plasmids (pGL3), and the corresponding 2`-O-methyl and phosphorothioate miRNA mimics representing bantam, miR-8, miR-31, miR-1989, miR-3479, or control mimics (scrambled or mismatched seeding region of these miRNA) (S8 Table). [score:1]
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2
[+] score: 21
Therefore, although little is known about the specific functions of several of these miRNAs (e. g. miR-31, miR-101, miR-200b, miR-10b, miR-460, miR-15b, miR-16 and miR203) during muscle development, the close relationship between their targets and myogenesis regulation demonstrates a potential role during muscle development. [score:6]
Seven (miR-101, miR-10a, miR-10b, miR-1677, let-7f, miR-31, and miR-205b) were expressed at higher levels in layers, and ten (miR-203, miR-200b, miR-16c, miR-15b, miR-15c, miR-460, miR-429, let-7c, miR-2188, and gga-miR-N2) were expressed at higher levels in broilers. [score:5]
LATS2, a putative target of gga-miR-31, encodes a protein that has been reported to regulate the size of myocytes in the heart negatively [67]. [score:4]
Greco and colleagues demonstrated that miR-31 was induced in dystrophic (mdx) mice and in Duchenne muscular dystrophy patients, and in newborn mice and newly formed myofibers during postischemic regeneration, suggesting that it could be important in pathophysiological pathways that regulate muscle responses to damage and regeneration [42]. [score:2]
Little is known about the functional roles of the remaining eight (miR-31, miR-101, miR-200b, miR-10b, miR-460, miR-15b, miR-16 and miR-203) during muscle development. [score:2]
Six of these miRNAs (miR-31, miR-10a, miR-10b, miR-16C and two let-7 members) have been implicated in skeletal muscle regeneration or development [39- 42]. [score:2]
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3
[+] score: 12
The most significantly differentially expressed were gga-miR-206 (3.5-fold), gga-miR-31 (2.5-fold), gga-miR-3535 (2.5-fold), gga-miR-17–3p (2.3-fold), gga-miR-429 (2.3-fold) and gga-miR-200b (2.2-fold), and in comparison, gga-miR-454 (-2.9-fold) and gga-miR-1b (-2.7-fold) were those mostly down-regulated in the fat line (Fig. 4). [score:6]
miR-22 can regulate the PTEN/AKT pathway and target HDAC6 [66– 68]; miR-206 and miR-1a can suppress hepatic lipogenesis [69]; miR-29b and miR-9 are involved in insulin sensitivity and diabetes [70, 71]; miR-31 and miR-32 participate in differentiation of stem cells into adipocyte and lipid metabolism in oligodendrocytes [72, 73]. [score:6]
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4
[+] score: 10
N1 gga-miR-199b 1.92 2.15E-04 Up gga-miR-6651-5p 1.95 9.19E-03 Up gga-miR-31-5p −2.28 3.36E-16 Down gga-miR-200b-5p −1.78 1.31E-08 Down gga-miR-365-2-5p −1.99 4.74E-06 Down gga-miR-211 −2.21 9.36E-06 Down gga-miR-204 −2.21 9.36E-06 Down gga-miR-200a-5p −1.21 1.40E-05 Down gga-miR-146a-5p −1.07 7.10E-04 Down gga-miR-1674 −2.79 1.20E-03 Down gga-miR-1552-5p −1.02 4.91E-03 Down Differentially expressed miRNA of each comparison were selected with a setting of q value < 0.01 and fold change > 2 The analysis of differentially expressed genes revealed a significant difference in skin tissues between early-feathering and late-feathering birds. [score:5]
Gga-miR-31-5p was the only miRNA that overlapped among all the possible comparisons, which was downregulated in F1 vs. [score:4]
L2 gga-miR-1c 2.69 8.53E-04 Up gga-miR-499-5p 2.64 1.73E-03 Up gga-miR-31-5p −1.14 1.95E-04 Down L2 vs. [score:1]
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5
[+] score: 8
In our study, gga-miR-202* was down-regulated by 3.2-fold and gga-miR-31 was down-regulated by 1.68-fold in sexually mature ovaries compared with immature ovaries, suggesting they are also involved in the sexual maturity of ovary. [score:6]
Studies indicated that gga-miR-31, gga-miR-101, gga-miR-202 and gga-miR-202* may be involved in regulating gonadal differentiation in embryonic chicken gonads [54- 56]. [score:2]
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6
[+] score: 3
Expression patterns of 15 miRNAs identified using RT-PCR agreed with those identified using deep sequencing, miR-101, miR-10a, miR-10b, miR-1677, let-7f, and miR-31 were higher in layers, while miR-200b, let-7c, miR-16c, miR15b, miR-15c, miR460, miR-429, miR-2188, and the novel miR-N2 were higher in broilers. [score:3]
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7
[+] score: 3
Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinicopathologic features of colorectal cancer. [score:3]
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8
[+] score: 3
MiR-31 negatively regulates expression of Fgf10, components of the Wnt and BMP signaling pathways, including sclerostin, BAMBI, and Dlx3 transcription factor, as well as selected keratin genes. [score:3]
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9
[+] score: 3
Other miRNAs from this paper: hsa-mir-31, mmu-mir-31, dre-mir-31
Furthermore, it was shown that in quiescent satellite cells, the mRNA is held together with miR31 in mRNP granules, preventing translation (Crist et al. 2012), and this mechanisms may also operate in the embryo. [score:3]
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10
[+] score: 3
In the discovery of Yamagishi et al., the Epi2miR pathway can be described as the regulatory process wherein under the control of EZH2 and SUZ12 (two important components of polycomb repressive complex), H3K9me and H3K27me histone modification regulates miR-31 and then affects NIK. [score:3]
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11
[+] score: 1
Of these, nine (gga-mir-1b, gga-mir-7, gga-mir-7b, gga-mir-10b, gga-mir-31, gga-mir-130b, gga-mir-204, gga-mir-215, gga-mir-489) are increased, and five (gga-mir-223, gga-mir-124b, gga-mir-140, gga-mir-183, gga-mir-222a) are decreased in CD30 [hi] cells. [score:1]
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12
[+] score: 1
Among them, moreover, 26 miRNAs (including 10 known miRNAs of miR-122, miR-1329-3p, miR-1587, miR-1736-3p, miR-1769-3p, miR-1769-5p, miR-1773-5p, miR-205a, miR-31 and miR-375) were found in all four comparisons (Table 3). [score:1]
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