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11 publications mentioning gga-mir-130b

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-130b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 113
Other miRNAs from this paper: gga-mir-29a, gga-mir-29b-1, gga-mir-124a, gga-mir-29c, gga-mir-29b-2
Gga-miR-130b-3p inhibited the expression level of MMP2 and MMP9, which indicates that this miRNA may be involved in MD tumor transformation through suppressing cell invasion. [score:7]
Overexpression of gga-miR-130b-3p inhibited cell proliferation, migration and invasion, indicating that this miRNA may function as a tumor suppressor to be involved in MD tumor transformation. [score:7]
The expression of TET2 was downregulated in tumorous spleens, which may contribute to the lower demethylation in the upstream region of gga-miR-130b-3p gene. [score:6]
Overexpression of gga-miR-130b-3p remarkably inhibited MSB1 cell proliferation at different time points. [score:5]
In GH3 rat pituitary tumor cells, miR-130b inhibited cell proliferation by arresting the cells in the G1 and G2 phase of the cell cycle through targeting cyclin A2 gene [65]. [score:5]
Our previous study investigated miRNA expression profiling between MD lymphoma and non-infected samples of chickens using Solexa deep sequencing, which showed that gga-miR-130b-3p expression was downregulated in MD lymphoma samples compared with that in non-infected ones. [score:5]
The expression level of gga-miR-130b-3p was significantly downregulated in tumorous spleens and livers compared with that in non-infected samples, respectively (Figure 1). [score:5]
Overexpression of miR-130b remarkably inhibited the invasive ability of pancreatic cancer cells [66]. [score:5]
MiR-130b functions as an oncogenic or tumor suppressor miRNA and shows dysregulation in various cancers including gastric, endometrial, and lung cancer [28– 30]. [score:4]
We verified that gga-miR-130b-3p was significantly downregulated in MDV-infected tumorous tissues. [score:4]
The hypermethylation may contribute to the downregulation of gga-miR-130b-3p in MDV-infected tumorous tissues. [score:4]
Expression level of gga-miR-130b-3p in non-infected spleen (NS), tumorous spleen (TS), non-infected liver (NL) and MD lymphoma from liver (LL) (n = 8). [score:3]
Gga-miR-130b-3p expression level was slightly lower in MDV-infected CEF cells [64], which was similar with our data. [score:3]
Gga-miR-130b-3p inhibited MSB1 cell proliferation, migration and invasion. [score:3]
Figure 1Expression level of gga-miR-130b-3p in non-infected spleen (NS), tumorous spleen (TS), non-infected liver (NL) and MD lymphoma from liver (LL) (n = 8). [score:3]
The MSB1 cells were transfected with the gga-miR-130b-3p agomir to stimulate miRNA overexpression. [score:3]
The overexpression of gga-miR-130b-3p prohibited the migration ability of MSB1 cells. [score:3]
To elucidate the reason for its differential expression and its function in MD tumorigenesis, we analyzed the methylation pattern of gga-miR-130b-3p gene and detected the effect of gga-miR-130b-3p on main features of MD lymphoblastoid cell line MDCC-MSB1. [score:3]
MiR-130b hypermethylation is observed in ovarian cancer tissues and demethylation leads to reactivation of miR-130b expression in drug resistant ovarian cancer cell lines [43]. [score:3]
Expression level of gga-miR-130b-3p in MDV-infected tumorous and non-infected tissues. [score:3]
Our previous study found gga-miR-130b-3p was abnormally expressed in MD tumorous tissues. [score:3]
MiR-130b arrested cell cycle at S phase and induced apoptosis in human pancreatic cancer PANC-2 and ASPC-1 cell lines, which resulted in suppression in cell proliferation both in vitro and in vivo [66]. [score:2]
MiR-130b inhibited cell migration and invasion in epithelial ovarian cancer cells [67]. [score:2]
Methylation level of each CpG site in the upstream region of gga-miR-130b-3p gene between MDV-infected tumorous and non-infected spleens. [score:1]
Figure 5mRNA level of MMP2 (A) and MMP9 (B) after transduction of the gga-miR-130b-3p agomir or NC (n = 3). [score:1]
In total of 1.5×10 [5] cells were seeded into 24-well plates and transfected with gga-miR-130b-3p agomir or agomir NC. [score:1]
The methylation pattern for the upstream 1 kb region of gga-miR-130b-3p gene was analyzed. [score:1]
The gga-miR-130b-3p agomir and corresponding NC were purchased from GenePharma Company (GenePharma Co. [score:1]
Effect of gga-miR-130b-3p on cell invasion. [score:1]
The SP1 binding site was located at the CpG site 24, 25, 26, 27 in the upstream region of gga-miR-130b-3p gene and their methylation rate was 0.575, 0.900, 0.800, 0.675, respectively in tumorous spleens. [score:1]
The upstream 1 kb region of gga-miR-130b-3p gene was determined using Ensembl Genome Browser (http://asia. [score:1]
The cells were seeded into 96-well plates (3×10 [4] cells/well) and transfected with gga-miR-130b-3p agomir or agomir NC. [score:1]
Figure 2 (A) Heat map depicting methylation pattern of forty-eight CpG sites in the upstream region of gga-miR-130b-3p gene between tumorous and non-infected spleens. [score:1]
Effect of gga-miR-130b-3p on cell proliferation and migration. [score:1]
Two amplicons were designed to cover the upstream region of precursor gga-miR-130b-3p. [score:1]
There were forty-eight CpG sites in the upstream region of gga-miR-130b-3p gene. [score:1]
Transcription factor binding sites that bind with the upstream region of gga-miR-130b-3p gene were predicted through online software. [score:1]
mRNA level of MMP2 (A) and MMP9 (B) after transduction of the gga-miR-130b-3p agomir or NC (n = 3). [score:1]
We analyzed the effects of gga-miR-130b-3p on tumorous cell features. [score:1]
The promoter CpG islands of miR-130b was not methylated in NSCLC [40]. [score:1]
The upstream region of gga-miR-130b-3p gene was hypermethylated in MDV-infected tumorous spleens. [score:1]
Each individual sample for gga-miR-130b-3p detection was run in triplicate. [score:1]
The protein level of MMP2 (C) and MMP9 (D) after transduction of the gga-miR-130b-3p agomir or NC (n = 3). [score:1]
SP1 has the function of preventing the gene promoter from methylation and thus SP1 might be prevented from binding to the promoter of gga-miR-130b-3p, which results in the methylation of this miRNA. [score:1]
The upstream region of gga-miR-130b-3p gene was hypermethylated in tumorous spleens. [score:1]
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2
[+] score: 10
For example, a comparison of miRNA expression profiles in proliferating myoblasts and differentiated myotubes revealed that miR-221 and miR-222 are down-regulated upon differentiation of primary and established myogenic cells, whereas miR-21, miR-103, miR-130, miR-99, miR-30 and miR20 are up-regulated [19, 33], suggesting that these miRNAs play important roles in the transition between proliferation and differentiation of muscle cells. [score:9]
In addition to miR-206, miR-1 and miR-181, nine other miRNAs among the most abundant in these libraries (miR-221, miR-222, miR-21, miR-103, miR-130, miR-99, miR-30, miR20, and miR128) have been implicated in the proliferation and differentiation of muscle cells (Table 1) [15, 19, 33]. [score:1]
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3
[+] score: 4
The expression of the following miRNAs was detected: gga-let-7i, gga-mir-130a, gga-mir-130b, gga-mir-199-2, gga-mir-1639, gga-mir-1a-2, gga-mir-124a, gga-mir-219, and gga-mir-310. [score:3]
7i: gga-let-7i, 130b:gga-mir-130b, 1639: gga-mir-1639, 199-2: gga-mir-199-2, 1a-2: gga-mir-1a-2, 124a: gga-mir-124a, 130a: gga-mir-130a, 219: gga-mir-219, 301: gga-mir-301. [score:1]
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4
[+] score: 4
Approximately 15.34% of the DEGs are targeted by at least two DEMs, and the LASP1 gene is regulated by seven DEMs (miR-24, miR-133a, miR-133b, let-7b, miR-20a, miR-20b and miR-130b). [score:4]
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5
[+] score: 4
However, overlap between our data and zebra-fish cardiac enriched transcripts was limited to the miR499 and the miR133 family [26], while the Zebra fish cardiac enriched miR221 and miR130b miRNA transcripts showed significantly lower expression in our chicken heart data compared to embryo expression levels. [score:4]
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6
[+] score: 4
Among these, gga-miR-216a, gga-miR-365-2-5p and gga-miR-130b-5p participate in epithelial cell differentiation through negatively regulating the expression of KRT6A, UPK1B and TCF21, respectively. [score:4]
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7
[+] score: 4
Additional data indicate that FGF and BMP signaling pathway interactions are regulated by negative feedback loops involving microRNAs, particularly miR-130 and miR-133 [48, 49]. [score:2]
Lopez-Sanchez C. Franco D. Bonet F. Garcia-Lopez V. Aranega A. Garcia-Martinez V. Negative fgf8-bmp2 feed-back is regulated by mir-130 during early cardiac specification Dev. [score:2]
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8
[+] score: 3
The present study of splenic miRNA and mRNA profiles from chickens after Salmonella challenge has identified differential expression of several miRNAs linked to immune responses, including miR-155, miR-9, miR-30 which have been reported previously and several miRNAs, such as miR-101-3p and miR-130b-3p, which were shown here to be associated with the immune response to infection with SE. [score:3]
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9
[+] score: 1
Of these, nine (gga-mir-1b, gga-mir-7, gga-mir-7b, gga-mir-10b, gga-mir-31, gga-mir-130b, gga-mir-204, gga-mir-215, gga-mir-489) are increased, and five (gga-mir-223, gga-mir-124b, gga-mir-140, gga-mir-183, gga-mir-222a) are decreased in CD30 [hi] cells. [score:1]
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10
[+] score: 1
Seven conserved families all were DE with P ≤ 0.05, including let-7 (let-7a, -7b, -7c,-7f, -7g, -7i, -7j, and -7k), miR-130 (miR-130a, and -130b), miR-146 (miR-146a, -146b, and -146c), miR-15 (miR-15a, -15b, and -15c), miR-181 (miR-181a and -181b), miR-29 (miR-29a, -29b and -29c), and miR-30 (miR-30a, -30b, -30c, -30d, and -30e). [score:1]
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11
[+] score: 1
However, many important candidate miRNAs related to lipid mechanism (e. g., gga-miR-301b-3p, gga-miR-130b-3p, gga-miR-30a-5p, gga-miR-142, gga-miR-146b, gga-miR-103, gga -miR-26a, etc. ) [score:1]
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