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19 publications mentioning gga-mir-146a

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-146a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 56
The relative expression of each Renilla luciferase target construct was compared between cells expressing miR-146a and those expressing the scrambled control sequence (SC) using a t-test for statistical significance (p < 0.05). [score:8]
Insertion of target sequences into psiCHECK-2. Construction of RCAS viruses expressing chicken miR-146a. [score:5]
The results are shown in Figure 4. The 3’ UTR of five (ARL11, CHMP2B, POU1F1, PDHB and HIF1AN) out of the seven genes targeted by miR-146a showed significant suppression of Renilla luciferase activity in RCAS-miR-146a infected cells relative to those infected with RCAS-SC (P < 0.05). [score:5]
The psiCHECK-2 construct (100 ng) for each potential target gene, as well as the scramble control, were then transfected into both RCAS- miR-146a or RCAS- SC infected DF-1 cells using FuGENE 6 (Promega, WI). [score:3]
Of particular note, gga-miR-146a is one of differentially expressed miRNAs that was associated with virus infection in both broiler (current study) and layer chickens [20]. [score:3]
The previous described RCASBP(A)-miR vector [49] was used to ectopically express miR-146a. [score:3]
Ectopic expression of the miR-146a was validated using a miScript Reverse Transcription kit and a miScript SYBR Green PCR kit (Qiagen, CA). [score:3]
CASP6, a gene containing no binding site for miR-146a but predicted to contain a miR-143 target site was used as negative control. [score:3]
For each predicted target gene a luciferase reporter vector was constructed in which the predicted miR-146a binding site was cloned into the 3’ UTR of a Renilla luciferase reporter gene. [score:3]
To produce miR-146a expressing viruses (RCAS- miR-146a), the RCASBP(A)-miR146a plasmid vector was transfected into DF-1 cells, a chicken embryo fibroblast continuous cell line, using FuGENE 6 (Promega, WI). [score:3]
Figure 4 Validation of miR-146a target genes in the Renilla luciferase reporter system. [score:3]
Seven potential miR-146a target genes predicted by the miRanda algorithm were chosen for validation. [score:3]
In order to produce RCAS viruses expressing chicken miR-146a, an entry vector was constructed using PAGE purified 76-nt forward and 68-nt reverse oligos (Invitrogen). [score:3]
Seven potential target genes (Table 5) of gga-miR-146a were picked for the validation by a dual luciferase reporter assay. [score:2]
A set of target genes containing miR-146a binding sites within their 3´UTRs were selected for further analysis using a dual luciferase reporter assay. [score:2]
For each potential target gene, the region of 3´UTR flanking the miR-146a binding sites was PCR amplified from Red Jungle Fowl genomic DNA using gene specific primers (Additional file 4: Table S4). [score:2]
The RCASBP(A)- miR-146a vector was generated via a recombination between the pENTR3C- miR-146a entry vector and RCASBP(A)-YDV gateway destination vector using a LR clonase kit (Invitrogen, CA). [score:1]
DF1 cells were infected with either RCAS-miR-146a or RCAS- SC at a multiplicity of infection of 1 and maintained for 6 days in a 96-well plate in RPMI 1640 medium supplemented with 1% heat-inactivated FBS, L-glutamine, penicillin (100 U/ml), streptomycin (100 μg/ml), and fungizone (4 μg/ml), at 37°C with 5% CO [2]. [score:1]
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2
[+] score: 27
Similarly, gga-miR-146a, upregulated in CD40L-stimulated B cells, was down-regulated in DT40 cells, providing further evidence for its role in the immune system. [score:7]
On the other hand, gga-miR-146a, which was upregulated in CD40L stimulated B cells, is down-regulated in DT40 cells, providing further evidence for its role in the immune system. [score:7]
Our study demonstrating the upregulation of miR-146a through CD40L interaction further adds to our understanding of the molecular pathways of biogenesis of miR-146a and CD40L functions. [score:4]
Another miRNA that shows increase in expression after CD40L-stimulated B-cells is gga-miR-146a. [score:3]
Role of miRNA-146a in the regulation of the innate immune response and cancer. [score:2]
Activation of miR-146a, thought to be through the NF-κ B pathway, has also shown to be important in the innate immune responses (Williams et al., 2008). [score:1]
The miRNAs which showed significant increase upon CD40L-stimulation included gga-miR-21, gga-miR-155, gga-miR-146a, gga-miR-20b, gga-miR-106, gga-miR-222 and gga-miR-22 (Figure 2). [score:1]
The miRNAs which showed significant increase upon CD40L-stimulation included gga-miR-21, gga-miR-155, gga-miR-146a, gga-miR-20b, gga-miR-106, gga-miR-222, and gga-miR-22. [score:1]
The emerging role of MIR-146A in the control of hematopoiesis, immune function and cancer. [score:1]
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3
[+] score: 26
For example, miR-146a down-regulates the expression of TRAF6 and IRAK1 to suppress the activity of the NF-κB signaling pathway and has a key role in suppressing tumorigenesis and tumor progression by inhibiting tumor cell migration and invasion (Taganov et al., 2006). [score:12]
miR-146 is an immune system regulator and plays a key role in regulating different types of diseases (Jopling et al., 2005). [score:5]
NF-κB -dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses. [score:5]
Divergent intracellular pathways regulate interleukin-1beta -induced miR-146a and miR-146b expression and chemokine release in human alveolar epithelial cells. [score:4]
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4
[+] score: 25
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-106a, hsa-mir-16-2, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-23b, hsa-mir-27b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-140, hsa-mir-125b-2, hsa-mir-136, hsa-mir-146a, hsa-mir-150, hsa-mir-206, hsa-mir-155, hsa-mir-181b-2, hsa-mir-106b, hsa-mir-302a, hsa-mir-34b, hsa-mir-34c, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-125b-2, gga-mir-155, gga-mir-222a, gga-mir-221, gga-mir-92-1, gga-mir-19b, gga-mir-20a, gga-mir-19a, gga-mir-18a, gga-mir-17, gga-mir-16-1, gga-mir-15a, gga-mir-1a-2, gga-mir-206, gga-mir-223, gga-mir-106, gga-mir-302a, gga-mir-181a-1, gga-mir-181b-1, gga-mir-16-2, gga-mir-15b, gga-mir-140, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-181b-2, gga-mir-181a-2, gga-mir-1a-1, gga-mir-1b, gga-let-7a-2, gga-mir-34b, gga-mir-34c, gga-let-7j, gga-let-7k, gga-mir-23b, gga-mir-27b, gga-mir-24, gga-mir-122-1, gga-mir-122-2, hsa-mir-429, hsa-mir-449a, hsa-mir-146b, hsa-mir-507, hsa-mir-455, hsa-mir-92b, hsa-mir-449b, gga-mir-146b, gga-mir-302b, gga-mir-302c, gga-mir-302d, gga-mir-455, gga-mir-367, gga-mir-429, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-1458, gga-mir-1576, gga-mir-1612, gga-mir-1636, gga-mir-449c, gga-mir-1711, gga-mir-1729, gga-mir-1798, gga-mir-122b, gga-mir-1811, gga-mir-146c, gga-mir-15c, gga-mir-449b, gga-mir-222b, gga-mir-92-2, gga-mir-125b-1, gga-mir-449d, gga-let-7l-1, gga-let-7l-2, gga-mir-122b-1, gga-mir-122b-2
Specifically, there was a 1.16 fold up-regulation (2.12 fold in deep sequencing analysis) in miR-1a, 2.13 fold down-regulation (8.33 fold in deep sequencing analysis) in miR-125b, and 3.03 fold down-regulation (3.45 fold in deep sequencing analysis) in miR-146a (P < 0.05). [score:10]
Based on other immune related miRNA studies in mammals [11, 66], differentially expressed miRNAs of their mammalian homologs and their targets are presented in Table 9. MiR-15a, miR-21 and miR-181a have important functions in lymphocytes development and modulations while miR-122 and miR-24 are related to virus infection and miR-146a, induced by macrophages, can activate Toll like receptor (TLR) and expose antigens to interleukin-1 beta. [score:6]
Interestingly, some of these differentially expressed miRNAs, including miR-146, have been previously reported to be associated with immune-related signal pathways in mammals. [score:3]
After exposure of THP-1 (human acute monocytic leukemia cell line) cells to lipopolysaccharides (LPS), miR-146 was identified as an inhibitor of signalling proteins of the innate immune responses by NF-kappaB [14]. [score:3]
There were general consistency between TaqMan assay and deep sequence analysis in three miRNAs (miR-1a, miR-125b and miR-146a) in terms of directions of regulation and significance. [score:2]
The expression levels of miR-1a, miR-125b and miR-146a in each sample were measured in terms of threshold cycle value and normalized to U6 using 2 [-ΔΔCT ][72]. [score:1]
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5
[+] score: 25
Other miRNAs from this paper: gga-mir-146b, gga-mir-21
We observed miRNA-146a expression to be increasingly up-regulated until 24 hours in TECs exposed to either R [low] or R [high] in contrast to the pattern observed upon exposure to LAMPs, which peaked at 6 hours and waned thereafter, likely due to the lack of LAMP re-stimulation [91]. [score:6]
For example, miRNA-146a, which is induced by LPS via NF-κB activation, down-regulates IRAK-1 and TRAF-6 and in turn suppresses further activation of NF-κB [90]. [score:6]
Expression analysis of miRNAs showed that miRNA146a increased over time in TECs exposed to live mycoplasmas; however in TECs exposed to the LAMPs from both strains, miRNA146a expression peaked at 6 hours (Figure 6B ). [score:5]
Expression analysis of four miRNA genes including mir-21, mir-146a, mir-146b, and mir-146c1, as well as IL-10 was assessed in TECs exposed to either LAMPs or live organisms. [score:3]
miRNA146a also negatively regulate TLR-2 signaling [91]. [score:2]
Therefore, miRNA-146a and in part IL-10 may play a role in regulating the over-exuberant pro-inflammatory response observed during M. gallisepticum infection. [score:2]
B: mRNA fold difference of miRNA-146a in TECs at all three time points post exposure. [score:1]
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6
[+] score: 12
For instance, few proteins (IRAK1, IRAK2, and TRAF6) within TLR signaling have been confirmed as direct targets of miR-146 (O'Neill et al., 2011); signal molecules MyD88, TAB2, SHIP1, and SOCS1 were targets of miR-155 (Eulalio et al., 2012); and cytokines IL-6 and IL-10 are targeted by Let-7 (Stae del and Darfeuille, 2013). [score:8]
Previous studies have shown that miRNAs, such as miR-146a, miR-155, and Let-7 and their targets are involved in the regulation of immune response to Salmonella or lipopolysaccharide infection in mice (O'Neill et al., 2011; Schulte et al., 2011; Eulalio et al., 2012) and swine (Bao et al., 2014, 2015; Yao et al., 2016a, b). [score:4]
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[+] score: 9
These miRNAs were divided into three groups according to their expression levels in the fat broiler line, 4 highly expressed (gga-miR-21, gga-miR-148a, gga-miR-103, gga-miR-101) (2 [-ΔCt] >0.7), 4 moderately expressed (gga-miR-100, gga-miR-146a, gga-miR-92, gga-miR-2188) (0.7>2 [-ΔCt]>0.08), and 7 lowly expressed (gga-miR-1a, gga-miR-130a, gga-miR-221, gga-miR-19a, gga-miR-181b, gga-miR-458, gga-miR-17–3p) (2 [-ΔCt]<0.08) (Table 2). [score:9]
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8
[+] score: 7
Other miRNAs from this paper: gga-mir-26a, gga-mir-223, gga-mir-126, gga-mir-451
For example, miR-146a has been shown to modulate the adaptive immune responses by regulating the IL-2 expression in human T lymphocytes [18]. [score:4]
However, chicken IL-2 is not a predicted target of gga-miR-146a http://www. [score:3]
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9
[+] score: 7
Other miRNAs from this paper: hsa-let-7d, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-21, hsa-mir-22, hsa-mir-30a, hsa-mir-32, hsa-mir-33a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-147a, hsa-mir-34a, hsa-mir-187, hsa-mir-204, hsa-mir-205, hsa-mir-200b, hsa-mir-23b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-138-2, hsa-mir-142, hsa-mir-144, hsa-mir-125b-2, hsa-mir-138-1, hsa-mir-146a, hsa-mir-190a, hsa-mir-200c, hsa-mir-155, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-365b, hsa-mir-328, gga-mir-33-1, gga-mir-125b-2, gga-mir-155, gga-mir-17, gga-mir-148a, gga-mir-138-1, gga-mir-187, gga-mir-32, gga-mir-30d, gga-mir-30b, gga-mir-30a, gga-mir-30c-2, gga-mir-190a, gga-mir-204-2, gga-mir-138-2, gga-let-7d, gga-let-7f, gga-mir-205b, gga-mir-200a, gga-mir-200b, gga-mir-34a, gga-mir-30e, gga-mir-30c-1, gga-mir-205a, gga-mir-204-1, gga-mir-23b, gga-mir-142, hsa-mir-449a, hsa-mir-489, hsa-mir-146b, hsa-mir-548a-1, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-33b, hsa-mir-449b, gga-mir-146b, gga-mir-147, gga-mir-489, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-144, gga-mir-460a, hsa-mir-147b, hsa-mir-190b, gga-mir-22, gga-mir-460b, gga-mir-1662, gga-mir-1684a, gga-mir-449c, gga-mir-146c, gga-mir-449b, gga-mir-2954, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, gga-mir-365b, gga-mir-33-2, gga-mir-125b-1, gga-mir-190b, gga-mir-449d, gga-mir-205c
The miR-146b and miR-146a these two members of miR-146 family, which negatively regulate expressions of inflammatory genes in some cell types containing airway smooth muscle, endothelial, epithelial cells and fibroblasts, are upregulated in a murine asthma mo del [34]. [score:7]
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10
[+] score: 7
Specifically, the gga-miR-146a is an only differentially expressed miRNAs with the criteria of FDR < 0.05 by targeting ESRP2, which indicated that miR-146a could influence the postnatal liver maturation process by targeting ESRP2 genes [3]. [score:7]
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11
[+] score: 5
The SDE miRNAs with high fold-changes were all down-regulated in L30, including miR-146b-5p (−8.50-fold), miR-24-3p (−7.39-fold), miR-146a-5p (−5.96-fold), miR-221-5p (−5.85-fold), miR-7b (−5.35-fold), miR-147 (−5.11-fold), miR-20-5p (−4.59-fold), and miR-140b-5p (−4.57-fold). [score:4]
Seven conserved families all were DE with P ≤ 0.05, including let-7 (let-7a, -7b, -7c,-7f, -7g, -7i, -7j, and -7k), miR-130 (miR-130a, and -130b), miR-146 (miR-146a, -146b, and -146c), miR-15 (miR-15a, -15b, and -15c), miR-181 (miR-181a and -181b), miR-29 (miR-29a, -29b and -29c), and miR-30 (miR-30a, -30b, -30c, -30d, and -30e). [score:1]
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12
[+] score: 5
N1 gga-miR-199b 1.92 2.15E-04 Up gga-miR-6651-5p 1.95 9.19E-03 Up gga-miR-31-5p −2.28 3.36E-16 Down gga-miR-200b-5p −1.78 1.31E-08 Down gga-miR-365-2-5p −1.99 4.74E-06 Down gga-miR-211 −2.21 9.36E-06 Down gga-miR-204 −2.21 9.36E-06 Down gga-miR-200a-5p −1.21 1.40E-05 Down gga-miR-146a-5p −1.07 7.10E-04 Down gga-miR-1674 −2.79 1.20E-03 Down gga-miR-1552-5p −1.02 4.91E-03 Down Differentially expressed miRNA of each comparison were selected with a setting of q value < 0.01 and fold change > 2 The analysis of differentially expressed genes revealed a significant difference in skin tissues between early-feathering and late-feathering birds. [score:5]
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13
[+] score: 4
MiR-21, miR-146a/b and miR-155 were obviously up-regulated in rat’s mononuclear cells after Salmonella infection [14, 15]. [score:4]
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14
[+] score: 3
Comer B. S. Camoretti-Mercado B. Kogut P. C. Halayko A. J. Solway J. Gerthoffer W. T. MicroRNA-146a and microRNA-146b expression and anti-inflammatory function in human airway smooth muscle Am. [score:3]
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15
[+] score: 2
MiR-146a functions in an anti-catabolic manner in articular cartilage by antagonizing the IL-1β -induced expression of cartilage-degrading enzymes MMP13 [51] and ADAMTS5 [52]. [score:2]
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16
[+] score: 2
[40] It was revealed that miR-146, [41] miR-155, [42] and miR-203 [43] regulate arthritic inflammatory response. [score:2]
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17
[+] score: 1
Moreover, the activation of TLR signals can also induce microRNAs (such as miR-146, miR-155 and miR-223) that can feedback the components in the TLR signalling system[9, 10]. [score:1]
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18
[+] score: 1
Similarly, other miRNA families exhibited vast read ranges, such as the gga-miR146 (91–235,844 reads) and gga-miR-30 (886–164,361 reads) families (Additional file 1: Table S2). [score:1]
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[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-mir-15a, hsa-mir-18a, hsa-mir-33a, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-107, mmu-mir-27b, mmu-mir-126a, mmu-mir-128-1, mmu-mir-140, mmu-mir-146a, mmu-mir-152, mmu-mir-155, mmu-mir-191, hsa-mir-10a, hsa-mir-211, hsa-mir-218-1, hsa-mir-218-2, mmu-mir-297a-1, mmu-mir-297a-2, hsa-mir-27b, hsa-mir-128-1, hsa-mir-140, hsa-mir-152, hsa-mir-191, hsa-mir-126, hsa-mir-146a, mmu-let-7a-1, mmu-let-7a-2, mmu-mir-15a, mmu-mir-18a, mmu-mir-103-1, mmu-mir-103-2, mmu-mir-342, hsa-mir-155, mmu-mir-107, mmu-mir-10a, mmu-mir-218-1, mmu-mir-218-2, mmu-mir-33, mmu-mir-211, hsa-mir-374a, hsa-mir-342, gga-mir-33-1, gga-let-7a-3, gga-mir-155, gga-mir-18a, gga-mir-15a, gga-mir-218-1, gga-mir-103-2, gga-mir-107, gga-mir-128-1, gga-mir-140, gga-let-7a-1, gga-mir-103-1, gga-mir-218-2, gga-mir-126, gga-let-7a-2, gga-mir-27b, mmu-mir-466a, mmu-mir-467a-1, hsa-mir-499a, hsa-mir-545, hsa-mir-593, hsa-mir-600, hsa-mir-33b, gga-mir-499, gga-mir-211, gga-mir-466, mmu-mir-675, mmu-mir-677, mmu-mir-467b, mmu-mir-297b, mmu-mir-499, mmu-mir-717, hsa-mir-675, mmu-mir-297a-3, mmu-mir-297a-4, mmu-mir-297c, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-467c, mmu-mir-467d, mmu-mir-466d, hsa-mir-297, mmu-mir-467e, mmu-mir-466l, mmu-mir-466i, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-467f, mmu-mir-466j, mmu-mir-467g, mmu-mir-467h, hsa-mir-664a, hsa-mir-1306, hsa-mir-1307, gga-mir-1306, hsa-mir-103b-1, hsa-mir-103b-2, gga-mir-10a, mmu-mir-1306, mmu-mir-3064, mmu-mir-466m, mmu-mir-466o, mmu-mir-467a-2, mmu-mir-467a-3, mmu-mir-466c-2, mmu-mir-467a-4, mmu-mir-466b-4, mmu-mir-467a-5, mmu-mir-466b-5, mmu-mir-467a-6, mmu-mir-466b-6, mmu-mir-467a-7, mmu-mir-466b-7, mmu-mir-467a-8, mmu-mir-467a-9, mmu-mir-467a-10, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, hsa-mir-466, hsa-mir-3173, hsa-mir-3618, hsa-mir-3064, hsa-mir-499b, mmu-mir-466q, hsa-mir-664b, gga-mir-3064, mmu-mir-126b, gga-mir-33-2, mmu-mir-3618, mmu-mir-466c-3, gga-mir-191
For example, a miRNA host transcript with a lincRNA biotype (ENSG00000253522) was merged between the Ensembl automatic pipeline and the Havana manual curation and was found to be given two names, CTC-231O11.1 or hsa-mir-146a. [score:1]
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