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5 publications mentioning cel-mir-360

Open access articles that are associated with the species Caenorhabditis elegans and mention the gene name mir-360. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 66
Based on fold-change analysis and statistical analysis, we identified 16 up-regulated miRNAs (mir-240, mir-75, mir-787, mir-62, mir-251, mir-252, mir-1821, mir-360, mir-353, mir-254, mir-229, mir-1824, mir-795, mir-1820, mir-41, and mir-4923b) and 4 down-regulated miRNAs (mir-4812, mir-53, mir-794, and mir-86) in nematodes infected with C. albicans SC5314 (Figs 1, 2a,b and 9, Table S2). [score:7]
The up-regulated miRNAs included mir-240, mir-75, mir-787, mir-62, mir-251, mir-252, mir-1821, mir-360, mir-353, mir-254, mir-229, mir-1824, mir-795, mir-1820, mir-41, and mir-4923b, while the down-regulated miRNAs included mir-4812, mir-53, mir-794, and mir-86 in C. albicans SC5314 infected nematodes (Fig. 2a,b). [score:7]
Consistent with the effects of mir-251, mir-252, and mir-360 mutations on lifespan and immune-response gene expression, mutation of mir-251 and mir-252 reduced C. albicans colony formation in the intestine, whereas mutation of mir-360 enhanced C. albicans colony formation in the intestine (Fig. 6). [score:6]
Meanwhile, after 4-h infection with C. albicans SC5314, we observed that mutation of mir-360 did not obviously influence the expression level of any examined antimicrobial gene (data not shown), implying that we should consider both the early and late immune response of nematodes during C. albicans infection. [score:4]
After infection with C. albicans, qRT-PCR assays showed that the expression levels of mir-251, mir-360, mir-252, mir-1821, mir-254, mir-62, mir-240, and mir-75 were significantly increased, and the expression levels of mir-86 and mir-53 were significantly decreased (Fig. 2c). [score:4]
After infection with C. albicans SC5314 for 36 h, loss-of-function mutation of mir-360 further caused the decrease in expression levels of abf-2, cnc-4, cnc-7, and fipr-22/23 relative to wild-type N2 nematodes (Fig. 5a). [score:4]
In contrast, after infection with C. albicans SC5314, mir-360 mutants showed lower expression levels of abf-2, cnc-4, cnc-7, and fipr-22/23 than wild-type N2 (Fig. 5a), suggesting that mutation of mir-360 may reduce the innate immune response to C. albicans SC5314. [score:4]
Using the available mutants, we found that mir-251 or mir-252 mutation renders nematodes resistant to C. albicans infection, whereas mir-360 mutation makes nematodes hypersensitive to C. albicans infection. [score:3]
In contrast, after infection for 24 h, mir-251 and mir-252 mutations significantly reduced the CFU per worm, whereas mir-360 mutation significantly increased the CFU per worm (Fig. 6c). [score:3]
In contrast, after infection with C. albicans SC5314 for 24 h, loss-of-function mutation of mir-360 resulted in the decreased expression levels of abf-2, cnc-4, and fipr-22/23 compared with wild-type N2 nematodes (Fig. 5a). [score:3]
Effect of candidate miRNAs on C. elegans lifespan after C. albicans infectionTo understand the function of our candidate miRNAs in in C. elegans’ immune response to C. albicans infection, we employed the available miRNA mutants (mir-251(n4606), mir-252(n4570), mir-254(n4470), mir-360(n4635), mir-240/786(n4541), mir-62(n4539), and mir-75(n4472)) to examine the effects of loss-of-function mutations of candidate miRNAs on C. elegans lifespan after C. albicans SC5314 infection. [score:2]
These data suggest that mutations in mir-251, mir-252, or mir-360 can influence the adverse effects of C. albicans infection on nematodes. [score:2]
In contrast, mutation of mir-360 increased the relative fluorescence intensity of CaSA1::GFP in both the proximal and the distal intestines in nematodes (Fig. 6b). [score:2]
Conversely, mutation of mir-360 exacerbated the accumulation of C. albicans cells in both the proximal and distal intestine in nematodes (Fig. 6a). [score:2]
To understand the function of our candidate miRNAs in in C. elegans’ immune response to C. albicans infection, we employed the available miRNA mutants (mir-251(n4606), mir-252(n4570), mir-254(n4470), mir-360(n4635), mir-240/786(n4541), mir-62(n4539), and mir-75(n4472)) to examine the effects of loss-of-function mutations of candidate miRNAs on C. elegans lifespan after C. albicans SC5314 infection. [score:2]
These results suggest that mir-251, mir-252, and mir-360 possibly play crucial roles in regulating the innate immune response of nematodes to C. albicans SC5314 infection. [score:2]
Our data suggest that mir-251, mir-252, and mir-360 play crucial roles in regulating the innate immune response to C. albicans infection. [score:2]
In contrast, the mir-360(n4635) mutant was hypersensitive to C. albicans SC5314 infection. [score:1]
Therefore, mir-251, mir-252, and mir-360 may alter C. albicans colony formation in the intestine of nematodes. [score:1]
Among the available mutants for candidate miRNAs, we found that mir-251 and mir-252 mutants were resistant to C. albicans SC5314 infection, whereas mir-360 mutants were hypersensitive to C. albicans SC5314 infection (Figs 4 and 9). [score:1]
After C. albicans SC5314 infection, statistical comparisons of the survival plots indicate that survival of the mir-251, mir-252, or mir-360 mutant was significantly different from that in wild-type N2 (P < 0.0001). [score:1]
C. elegans strains used in this study were wild-type N2, mir-251(n4606), mir-252(n4570), mir-254(n4470), mir-360(n4635), mir-240/786(n4541), mir-62(n4539), and mir-75(n4472). [score:1]
C. albicans strain of CaSA1 was fed for 16 h. (c) Comparison of colony-forming units (CFU) between wild-type N2 and mir-251, mir-252, or mir-360 mutant. [score:1]
Under normal conditions, the mir-251(n4606), mir-252(n4570), and mir-360(n4635) mutants had similar lifespan to wild-type N2 nematodes (Fig. S4). [score:1]
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2
[+] score: 4
Other miRNAs from this paper: cel-mir-231
Another dual regulation mechanism between mir-360 and CEP-1 in the control of GO induced germline apoptosis was recently identified in the gonads, a secondary targeted organ of GO in nematodes 29. [score:4]
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3
[+] score: 3
Among the dysregulated miRNAs, the further bioinformatics analysis demonstrated that lin-4, mir-228, mir-249, mir-47, mir-355, mir-45, mir-2210, mir-57, mir-1018, mir-360, mir-64, mir-2209, mit-793, mir-1830, mir-2210, mir-83, mir-789, and mir-806 might be involved in the control of MWCNTs toxicity through affecting the functions of identified dysregulated genes in exposed nematodes (Table S6). [score:3]
[1 to 20 of 1 sentences]
4
[+] score: 1
Other miRNAs from this paper: cel-let-7, cel-mir-84, cel-mir-233, cel-mir-241, cel-mir-251, cel-mir-252
Recently, some miRNAs such as mir-84, mir-241, mir-233, mir-251, mir-252, and mir-360 have been shown to be involved in the control of innate immunity in C. elegans [15– 17]. [score:1]
[1 to 20 of 1 sentences]
5
[+] score: 1
Other miRNAs from this paper: cel-mir-259, cel-mir-355
Similarly, previous study has demonstrated that the activated mir-360 may encode a protection mechanism for nematodes against the toxic effects of graphene oxide (GO) in inducing germline apoptosis 25. [score:1]
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