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6 publications mentioning dme-mir-307a

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-307a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 122
Consistent with this, expression of senseless (Sens), a short-range target gene of Wg signaling 31, is reduced by miR-307a overexpression (Fig. 1d-d’). [score:7]
Through Bip staining, we found that overexpression of miR-307a by hhGal4 caused ectopic expression of Bip specifically in the wg -expressing cells of the posterior wing disc (Fig. 1i-i”’). [score:7]
Moreover, overexpression of wls suppressed the ectopic Bip pattern induced by miR-307a overexpression (Fig. 2f-f”’). [score:7]
We found that knockdown of wg suppressed the ectopic expression of Bip induced by miR-307a (Fig. 3a-a’). [score:6]
Intriguingly, we found that overexpression of miR-307a induces ER stress specifically in the wg -expressing cells. [score:5]
MiR-307a initiates ER stress through targeting of wlsTo investigate the possible correlation between miR-307a and ER stress, we searched for targets of miR-307a using miRanda and TargetScan 32 33. [score:5]
The arrowheads indicate the loss of Sens expression in the miR-307a -expressing P compartment. [score:5]
In contrast to accumulated Wg within its expressing cells, extracellular Wg levels were reduced with miR-307a overexpression (Fig. 1c). [score:5]
In the wing disc, overexpression of miR-307a using enGal4 blocked expression of wls-sensor in the P compartment (Fig. 2c-c’). [score:5]
In this study, we identified that miR-307a inhibits Wg secretion through targeting wls. [score:5]
Overexpression of miR-307a resulted in accumulation of Wg in its expressing cells (Fig. 1b-b’). [score:5]
MiR-307a regulates Wg secretion and initiates ER stress in wg -expressing cellsTo identify new regulators of Wg secretion, we performed a genetic screen which mainly focuses on vesicle trafficking related proteins and a microRNA library described previously 30. [score:4]
miR-307a -induced ectopic Bip staining was suppressed by wg knockdown (arrowhead in a). [score:4]
Further, overexpression of miR-307a-sponge enhanced the signal of miR-307a-sensor (Supplementary Fig. S2d-d’). [score:3]
We found wls is one of the predicted targets of miR-307a (Fig. 2a). [score:3]
Subsequently, we generated the miR-307a-sensor (Supplementary Fig. S2b-b”) and miR-307a-sponge (Supplementary Fig. S2c) transgenes for monitoring the endogenous expression levels of miR-307a. [score:3]
MiR-307a regulates Wg secretion and initiates ER stress in wg -expressing cells. [score:3]
Comparing with the wild-type control (Supplementary Fig. S2a), the miR-307a-sensor shows decreased expression in the wing pouch with striking reduction at the A/P and D/V boundaries (Supplementary Fig. S2b-b”). [score:3]
Next, we asked whether miR-307a triggered ER stress through targeting of wls. [score:3]
Consistently, overexpression of miR-307a causes a remarkable reduction in Wls protein levels (Fig. 2d-d’). [score:3]
This pattern is similar with that of wls-sensor, indicating that miR-307a is endogenously expressed in these regions. [score:3]
To investigate the possible correlation between miR-307a and ER stress, we searched for targets of miR-307a using miRanda and TargetScan 32 33. [score:3]
These data demonstrated that wls is a target of miR-307a. [score:3]
In the screen, we identified that ectopic expression of miR-307a resulted in wing notches (Supplementary Fig. S1b-b’) and loss of dorsal thoracic bristles (Supplementary Fig. S1c-c’, red box in S1c’), raising the possibility that Wg signaling is disrupted. [score:3]
MiR-307a initiates ER stress through targeting of wls. [score:2]
MiR-307a initiates ER stress through targeting Wls. [score:2]
Interestingly, we found that miR-307a regulates the initiation of ER stress as indicated by Bip staining. [score:2]
Together, our data demonstrate that miR-307a is a negative regulator of Wg secretion. [score:2]
Forward: 5′-CGGCTAGCCGGAACGAGGATTCTG-3′ Reverse: 5′-CGGCTAGCCCTGATGGTTTAAGTCCTG-3′ To generate the pWALIUM10-moe-DsRed-miR-307a-sponge, the following sequence was designed as described in 47, and then directly cloned into pWALIUM10-moe-DsRed through BglII and NdeI sites. [score:2]
Taken together, these data suggested that miR-307a acts as an endogenous regulator in the wing pouch. [score:2]
Vps35 knockdown mimicked the Bip induced phenotype caused by miR-307a (Fig. 3b-b’). [score:2]
Forward: 5′-CGGCTAGCCGGAACGAGGATTCTG-3′ Reverse: 5′-CGGCTAGCCCTGATGGTTTAAGTCCTG-3′To generate the pWALIUM10-moe-DsRed-miR-307a-sponge, the following sequence was designed as described in 47, and then directly cloned into pWALIUM10-moe-DsRed through BglII and NdeI sites. [score:2]
UAS-GFP-miR-307a, UAS-DsRed-miR-307a-sponge, UAS-wg, UAS-wg(K [334] K > R [334] R), tub-EGFP, tub-EGFP-miR-307a-sensor and tub-DsRed-wls-3′ UTR transgenes were generated in this study. [score:1]
Next, we examined the role of miR-307a in Wg signaling in the wing imaginal discs. [score:1]
To generate the pWALIUM10-moe-GFP-GPI-miR-307a, 714 bp of genomic DNA surrounding miR-307a was amplified by PCR and cloned downstream of GFP-GPI in the NheI site of pWALIUM10-moe-GFP-GPI vector 30. [score:1]
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2
[+] score: 26
As shown in the Fig. 6A, sfr-mir-307-3p is highly expressed in haemolymph and expressed less in whole gut while there is a moderate to significant increase in the expression levels of sfr-mir-305-5p and Novel_miR-17 in the whole gut respectively. [score:7]
As shown in the Fig. 6B, there is no significant change in distribution of expression in case of sfr-mir-305-5p and Novel_miR-17 while there is a moderate change in distribution of expression in case of sfr-mir-307-3p. [score:5]
In addition to sfr-mir-305-5p, sfr-mir-307-3p displayed an increase of 2 fold and more in its expression levels in 6 [th] instar larvae. [score:3]
In addition, we performed the expression check for sfr-mir-305-5p, sfr-mir-307-3p and Novel_miR-17 in three segments of the whole gut, i. e., fore gut, mid gut and hind gut. [score:3]
Particularly, sfr-mir-307-3p levels are less in midgut while the expression is mostly constrained to the foregut followed by hindgut. [score:3]
The analysis revealed that, some of the known miRs (sfr-mir-305-5p, sfr-mir-307-3p) are expressed in a wide range of insect species (A. gambie, Culex sp, A. aegypti, A. mellifera, B. mori, T. castenum, D. melanogaster) while some (sfr-mir-71-3p) are restricted to a few (A. aegypti, B. mori, T. castenum). [score:3]
The miRs (sfr-mir-305-5p, sfr-mir-307-3p, sfr-mir-71-3p, sfr-mir-281, sfr-mir-317, sfr-mir-2756, sfr-mir-932, sfr-mir-184-3p, sfr-mir-2766, Novel_miR15, Novel_miR16, and Novel_miR17) were easily detectable in total RNA isolated from Sf21 cells (Fig. 4). [score:1]
As shown in Fig. 6C, Novel_miR-15 is highly expressed when compared to the other miRs, and sfr-mir-307-3p is approximately 2 fold higher compared to sfr-mir-305-5p. [score:1]
[1 to 20 of 8 sentences]
3
[+] score: 12
After controlling for the generation of adventitious target sites in the luciferase vector for other microRNAs expressed in S2 cells, only six microRNAs are found to repress their targets at levels greater than expected (bantam-5p, miR-33-5p, miR-8-3p, miR-307a-3p, miR-308-5p and miR-8-3p). [score:7]
Just four of these cases (miR-307a-5p, miR-79-3p, miR-970-5p and miR-308-3p) are targeted by other microRNAs that are expressed at similar or higher levels than the microRNA for which the assay was designed. [score:4]
The level of repression of five low abundance mature microRNAs (let-7-3p, miR-307a-5p, miR-970-5p, miR-1003-5p, miR-34-3p) is not significantly different from the empty control vector (paired t-test; p<0.05). [score:1]
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4
[+] score: 4
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-27a, hsa-mir-29a, hsa-mir-101-1, dme-mir-1, dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-10, mmu-let-7g, mmu-let-7i, mmu-mir-1a-1, mmu-mir-101a, mmu-mir-124-3, mmu-mir-126a, mmu-mir-133a-1, mmu-mir-137, mmu-mir-140, mmu-mir-142a, mmu-mir-155, mmu-mir-10b, mmu-mir-183, mmu-mir-193a, mmu-mir-203, mmu-mir-143, hsa-mir-10a, hsa-mir-10b, hsa-mir-34a, hsa-mir-183, hsa-mir-199b, hsa-mir-203a, hsa-mir-210, hsa-mir-222, hsa-mir-223, dme-mir-133, dme-mir-34, dme-mir-124, dme-mir-79, dme-mir-210, dme-mir-87, mmu-mir-295, mmu-mir-34c, mmu-mir-34b, mmu-let-7d, dme-let-7, dme-mir-2c, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-137, hsa-mir-140, hsa-mir-142, hsa-mir-143, hsa-mir-126, hsa-mir-193a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-29a, mmu-mir-27a, mmu-mir-34a, mmu-mir-101b, hsa-mir-1-1, mmu-mir-1a-2, hsa-mir-155, mmu-mir-10a, mmu-mir-210, mmu-mir-223, mmu-mir-222, mmu-mir-199b, mmu-mir-124-1, mmu-mir-124-2, hsa-mir-101-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-378a, mmu-mir-378a, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, mmu-mir-411, hsa-mir-193b, hsa-mir-411, mmu-mir-193b, hsa-mir-944, dme-mir-193, dme-mir-137, dme-mir-994, mmu-mir-1b, mmu-mir-101c, hsa-mir-203b, mmu-mir-133c, mmu-let-7j, mmu-let-7k, mmu-mir-126b, mmu-mir-142b, mmu-mir-124b
A recent study has shown that Drosophila Dicer-1, in association with various alternatively spliced isoforms of Dicer partner protein, loquacious (loqs), is capable of adjusting the length of mature miRNAs on pre-miR-307a, leading to the generation of 5′-isomiRs with distinct target specificity (23). [score:3]
Besides, a recent study has showed that Dicer can tune the cleavage sites on miR-307a precursor in fruitfly by associating with various loqs protein isoforms, leading to the generation of 5′ end miRNA isoforms of various lengths (23). [score:1]
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5
[+] score: 2
Our data show that the most abundant miRNA, miR-9a-5p, is about 12,000-fold more abundant than the levels of the least abundant miRNA reliably detected (miR-307a-3p). [score:1]
A total of 109 mature miRNAs were detected (with >1 read per sample), with the most abundant miRNA, miR-9a-5p, roughly 12,000-fold more abundant than the levels of the least abundant miRNA reliably detected (miR-307a-3p). [score:1]
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6
[+] score: 1
37), thus leaving 4% as conclusive false positives (miR-14, miR-79, miR-307 and miR-975; dark blue in Fig. 2b and ). [score:1]
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