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6 publications mentioning cel-mir-246

Open access articles that are associated with the species Caenorhabditis elegans and mention the gene name mir-246. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 63
Other miRNAs from this paper: cel-lin-4, cel-mir-66, cel-mir-71, cel-mir-239a, cel-mir-239b
Specifically, we here find clear evidence that elevated levels of mir-71 and mir-246 expression are associated with extended longevity, yet in daf-2 animals these miRNAs are not upregulated, likely reflecting their upstream, negative-regulatory roles in insulin signaling [36]. [score:7]
Given this, the relatively late (i. e. post-reproductive) timing of its predictive ability, the sharp upregulation of mir-246::GFP expression at reproductive maturity (Figure 5A, 5B and [47]), and its localization literally at the interface between the gonad and other somatic tissues, it is tempting to speculate that mir-246 is involved in balancing reproduction and somatic maintenance [32], [59]. [score:6]
The expression of miR-246 increases over time, and a mir-246::GFP construct shows that the gene is expressed in the gonadal sheath [36], [47]. [score:5]
None of the principal components of spatial expression variability correlate with longevity: mir-246::GFP expression is highly tissue-specific, and so the principal components predominantly capture uninteresting variations in the internal position of the gonad sheath. [score:5]
Many miRNAs change expression levels during aging in C. elegans [35], and recently mir-71, mir-239, and mir-246, all of which increase in expression over time, have been shown to promote (mir-71, mir-246) and antagonize (mir-239) longevity and stress-resistance, through IIS (mir-71, mir-239) and the DNA damage response pathway (mir-71) [36]. [score:5]
fast-increasing mir-246::GFP expression are significantly different (Figure S2E), they have nearly identical modal values; however, a subset with particularly early mortality appears to be associated with low mir-246::GFP slopes. [score:3]
Changes in mir-246::GFP and mir-239::GFP expression over time predict longevity. [score:3]
Including mir-246 decreases the link between length and lifespan (Figure S6 and Table S2), suggesting that, while its precise position is indeterminate, mir-246 is either an upstream cause of both length and lifespan variation, or mir-246 levels are determined by physiological processes that regulate length maintenance, and these levels more directly control lifespan. [score:3]
While mir-71 and mir-246 levels peak or plateau, respectively, at mid-life (Figure 3A, 3B and Figure 5A, 5B), we observe that mir-239::GFP expression levels drift upward over time (Figure 5D and 5E). [score:3]
1002306.g005 Figure 5Changes in mir-246::GFP and mir-239::GFP expression over time predict longevity. [score:3]
Our detailed analysis of mir-246::GFP in individual animals shows a gradual plateauing of mir-246 expression in late adulthood (Figure 5A and 5B), but, unlike mir-71, no concomitant loss of tissue specificity. [score:3]
Shorter-lived animals have, in general, more rapidly declining levels of mir-246::GFP expression and slightly more rapidly increasing levels of mir-239::GFP. [score:3]
As with mir-246::GFP, PCA applied to mir-239::GFP images did not yield any expression-pattern trends predictive of future longevity. [score:3]
Rates of mir-246/ mir-239 Increase Promote/Antagonize Individual LongevityMuch like mir-71, mir-246 mutants appear phenotypically wild-type except for decreased longevity and stress-resistance [36], [46]. [score:1]
The mean level of mir-246::GFP between days 3 and 7, however, does not clearly predict longevity. [score:1]
We find that animals in which mir-246::GFP levels plateau more slowly (measured by the slope of mir-246::GFP 95 [th]-percentile fluorescence intensity between days 3 and 7) are relatively longer-lived: change in mir-246 expression in this time range predicts 20% of total longevity variation (l. o. o. 18%; Figure 5B and 5C). [score:1]
The following C. elegans strains provided by Caenorhabditis Genetics Center (CGC) were used in our studies: VT2084 (mir-71::GFP), VT1607 (mir-246::GFP), and PD4793 (mIs10: myo-2::GFP; pes-10::GFP; F22B7.9::GFP). [score:1]
Unlike mir-71 and mir-246, mir-239 antagonizes longevity: mutants lacking the identical mir-239a and mir-239b sequences have increased lifespan and stress-resistance (though, again, no other clear phenotypes) [36], [46]. [score:1]
Based on this analysis, we observe that the various predictors of longevity differ markedly in their timing: the mir-71 PCA scores provide the earliest, and best, predictors of lifespan, while mir-246 and motion provide the latest-onset information about future lifespan. [score:1]
Note that the low- mir-246::GFP-slope cohort has a bimodal lifespan distribution: some are nearly as long-lived as the high-slope cohort, while others have a markedly shortened lifespan. [score:1]
Here we report that mir-71, mir-239, and mir-246 expression profiles, measured by promoter::GFP reporter constructs, predict individual longevity in C. elegans. [score:1]
Rates of mir-246/ mir-239 Increase Promote/Antagonize Individual Longevity. [score:1]
However, while both mir-71 and mir-246 levels are correlated with length maintenance, mir-246, unlike mir-71, provides additional information about longevity not captured by length or other “somatic maintenance” features. [score:1]
Much like mir-71, mir-246 mutants appear phenotypically wild-type except for decreased longevity and stress-resistance [36], [46]. [score:1]
[1 to 20 of 24 sentences]
2
[+] score: 26
Another miRNA found to be upregulated was miR-246-3p, that has been suggested to promote longevity, since mutants in this miRNA display a shorter lifespan and its overexpression leads to increased lifespan [81]. [score:6]
Although the targets of miR-246-3p have not been identified, aakg-1 (one of the five gamma subunits of AMP kinase) and lin-37 (involved in larval, embryo and vulval development) have been predicted as targets. [score:6]
We observed that the expression of miR-240-5p and miR-246-3p was upregulated in 12-hr starved worms, as seen in the RNA-seq experiments (Fig 7A). [score:6]
miR-240-5p, miR-246-3p, and the miR-35-41 cluster were upregulated under starvation conditions. [score:4]
For miR-240-5p, miR-246-3p, miR-35-3p, miR-36-3p, miR-39-3p quantification, miR-58-3p was used as a control. [score:1]
Relative quantification of (A) miR-240-5p, miR-246-3p, and (B) miR-35-3p, miR-36-3p, miR-39-3p, and gld-1 and lin-23 mRNA abundance in well-fed and 12-hr starved early L4 larvae by. [score:1]
The quantification of miR-35-3p, miR-36-3p, miR-39-3p, miR-240-5p, and miR-246-3p expression relative to miR-58-3p was calculated as in Pfaffl, 2001[47]. [score:1]
For Reverse Transcription of miRNAs (miR-35-3p, miR-36-3p, miR-39-3p, miR-240-5p, miR-246-3p, and miR-58-3p) and mRNAs (gld-1, lin-23 and β-actin), a total of 2000 ng and 300 ng were used, respectively. [score:1]
[1 to 20 of 8 sentences]
3
[+] score: 6
Given its temporal regulation, this miRNA might be involved in control of developmental timing, like lin-4 and let-7. Another interesting case is the expression of miR-77, miR-85, miR-240 and miR-246, which is very low or completely absent in earlier developmental stages but increases after the mid-L4 and young adult stages (Figure 3b; 4 and 5), implying a potential role in adult functions like reproduction, metabolism or aging. [score:6]
[1 to 20 of 1 sentences]
4
[+] score: 6
Other miRNAs from this paper: cel-lin-4, cel-mir-71, cel-mir-239a, cel-mir-239b
Deletions of miR-71 and miR-246 decrease nematode lifespan, and deletion of miR-239 increases lifespan, while overexpression of these miRNAs has the opposite effects. [score:3]
In addition, mir-71, mir-239, and mir-246, which each increase significantly in expression during aging in wild-type C. elegans, have longevity and stress-resistance phenotypes [136]. [score:3]
[1 to 20 of 2 sentences]
5
[+] score: 1
Loss of certain miRNAs or miRNA families led to hypoxia sensitivity (mir-2, mir-35, mir-44, mir-49, mir-51, mir-60, mir-63 and mir-67) and others to hypoxia resistance (let-7, mir-58, mir-67, mir-79, mir-237, mir-246, mir-359). [score:1]
[1 to 20 of 1 sentences]
6
[+] score: 1
In some instances loss of specific miRNAs (lin-4, miR-71, miR-238, miR-246 or miR-228) has resulted in shortened lifespan, whereas in others (miR-80 or miR-239a/b) lifespan extension has been observed [18– 23]. [score:1]
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