Stem-loop sequence mmu-mir-421

Accession

MI0005496 (change log)

Symbol

MGI:Mir421

Description

Mus musculus miR-421 stem-loop

Gene family

MIPF0000098; mir-95

Literature search

19 open access papers mention mmu-mir-421
(40 sentences)

Stem-loop

   -  uu      -    uua            a   aaa 
5'  ca  guaggc cuca   aauguuuguuga uga   a
    ||  |||||| ||||   |||||||||||| |||   a
3'  gu  cguccg gggu   uuacagacaacu acu   u
   u  cu      c    uaa            -   aag 

Deep sequencing

27136 reads, 32.7 reads per million, 104 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chrX: 103572921-103572996 [-]
• Overlapping transcripts:

  sense

  OTTMUST00000044290 ; Thcytx-002; intron 9

  OTTMUST00000044291 ; Thcytx-003; intron 10

  OTTMUST00000044293 ; Thcytx-005; intron 10

  OTTMUST00000044239 ; Thcytx-001; intron 10

  ENSMUST00000083575 ; Mir421-201; exon 1

  ENSMUST00000130063 ; Ftx-002; intron 9

  ENSMUST00000156211 ; Ftx-001; intron 10

  ENSMUST00000145803 ; Ftx-005; intron 10

  ENSMUST00000156240 ; Ftx-003; intron 10

Clustered miRNAs

< 10kb from mmu-mir-421

• mmu-mir-374c: chrX: 103573085-103573133 [+]
• mmu-mir-374b: chrX: 103573060-103573154 [-]
• mmu-mir-421: chrX: 103572921-103572996 [-]

Database links

• EntrezGene:Mir421
• MGI:Mir421
• RNAcentral:URS000075DE0F_10090
• miRBase Tracker:MI0005496

Mature sequence mmu-miR-421-5p

• Accession: MIMAT0017273
• Previous IDs: mmu-miR-421*
• Sequence: 11 - cucauuaaauguuuguugaau - 31
• Deep sequencing: 121 reads, 40 experiments
• Evidence: experimental; Illumina [3]
• Database links:
  • RNAcentral:URS000058723A_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-421-5p
  • miRDB:mmu-miR-421-5p

Mature sequence mmu-miR-421-3p

• Accession: MIMAT0004869
• Previous IDs: mmu-miR-421
• Sequence: 45 - aucaacagacauuaauugggcgc - 67
• Deep sequencing: 27011 reads, 102 experiments
• Evidence: experimental; cloned [1], Illumina [2-3]
• Database links:
  • RNAcentral:URS00004505E4_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-421-3p
  • miRDB:mmu-miR-421-3p
  • microrna.org:mmu-miR-421-3p

References

1. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
2. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
3. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).