Stem-loop sequence mmu-mir-199b

Accession

MI0000714 (change log)

Symbol

MGI:Mir199b

Description

Mus musculus miR-199b stem-loop

Gene family

MIPF0000040; mir-199

Literature search

77 open access papers mention mmu-mir-199b
(375 sentences)

Stem-loop

   cca    -auacc    cu  -     c       u        c   u  ----  a 
5'    gagg      ucca  cc gucua ccagugu uagacuac ugu ca    gg c
      ||||      ||||  || ||||| ||||||| |||||||| ||| ||    ||  
3'    cucc      gggu  gg cggau gguuaca gucugaug aca gu    cc u
   -gg    cagauu    cg  u     u       c        -   u  uaaa  c 

Deep sequencing

4069977 reads, 1.69e+04 reads per million, 106 experiments

Confidence

Annotation confidence: high

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Comments

Mouse miR-199b is predicted based on homology with the previously identified human miR-199b (MI0000282) [1,2]. Landgraf et al. later showed that the 3' product is the predominant one [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr2: 32318460-32318569 [+]
• Overlapping transcripts:

  antisense

  OTTMUST00000031111 ; Dnm1-013; intron 1

  OTTMUST00000031103 ; Dnm1-005; intron 14

  OTTMUST00000031101 ; Dnm1-003; intron 15

  OTTMUST00000031100 ; Dnm1-002; intron 15

  OTTMUST00000031102 ; Dnm1-004; intron 15

  OTTMUST00000031099 ; Dnm1-006; intron 15

  OTTMUST00000031104 ; Dnm1-001; intron 15

  ENSMUST00000129156 ; Dnm1-013; intron 1

  ENSMUST00000091089 ; Dnm1-005; intron 14

  ENSMUST00000078352 ; Dnm1-003; intron 15

  ENSMUST00000113352 ; Dnm1-002; intron 15

  ENSMUST00000113350 ; Dnm1-004; intron 15

  ENSMUST00000139624 ; Dnm1-006; intron 15

  ENSMUST00000113365 ; Dnm1-001; intron 15

Clustered miRNAs

< 10kb from mmu-mir-199b

• mmu-mir-3154: chr2: 32318265-32318343 [+]
• mmu-mir-199b: chr2: 32318460-32318569 [+]

Database links

• EntrezGene:Mirn199b
• MGI:Mir199b
• RNAcentral:URS000075997E_10090
• miRBase Tracker:MI0000714

Mature sequence mmu-miR-199b-5p

• Accession: MIMAT0000672
• Previous IDs: mmu-miR-199b;mmu-miR-199b*
• Sequence: 26 - cccaguguuuagacuaccuguuc - 48
• Deep sequencing: 438831 reads, 91 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00004DECC9_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-199b-5p
  • miRDB:mmu-miR-199b-5p
  • microrna.org:mmu-miR-199b-5p

Mature sequence mmu-miR-199b-3p

• Accession: MIMAT0004667
• Previous IDs: mmu-miR-199b
• Sequence: 65 - acaguagucugcacauugguua - 86
• Deep sequencing: 3631119 reads, 106 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00003F2D94_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-199b-3p
  • miRDB:mmu-miR-199b-3p
  • microrna.org:mmu-miR-199b-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).