Stem-loop sequence hsa-mir-218-2

Accession

MI0000295 (change log)

Symbol

HGNC:MIR218-2

Description

Homo sapiens miR-218-2 stem-loop

Gene family

MIPF0000026; mir-218

Literature search

177 open access papers mention hsa-mir-218-2
(1065 sentences)

Stem-loop

   gac   --uc  u    g       uuu         cu        gguggaacga  g 
5'    cag    gc gcgg gcuuucc   gugcuugau  aaccaugu          ug a
      |||    || |||| |||||||   |||||||||  ||||||||          ||  
3'    guc    cg ugcc cgaaagg   cacgaacug  uugguaca          gc a
   -ac   cucu  -    a       cgc         uc        --------ag  a 

Deep sequencing

349966 reads, 1.08e+03 reads per million, 145 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and the 5' end mapped by PCR. Landgraf et al. confirm expression in human [2].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr5: 168768146-168768255 [-]
• Overlapping transcripts:

  intergenic

Database links

• EntrezGene:MIR218-2
• HGNC:MIR218-2
• RNAcentral:URS00003735F0_9606
• miRBase Tracker:MI0000295

Mature sequence hsa-miR-218-5p

• Accession: MIMAT0000275
• Previous IDs: hsa-miR-218
• Sequence: 25 - uugugcuugaucuaaccaugu - 45
• Deep sequencing: 715721 reads, 142 experiments
• Evidence: experimental; cloned [2-3]
• Database links:
  • RNAcentral:URS000020D84A_9606
• Predicted targets:
  • TargetMiner:hsa-miR-218-5p
  • TargetScanVert:hsa-miR-218-5p
  • miRDB:hsa-miR-218-5p
  • microrna.org:hsa-miR-218-5p

Mature sequence hsa-miR-218-2-3p

• Accession: MIMAT0004566
• Previous IDs: hsa-miR-218-2*
• Sequence: 67 - caugguucugucaagcaccgcg - 88
• Deep sequencing: 1597 reads, 69 experiments
• Evidence: experimental; cloned [2]
• Predicted targets:
  • TargetMiner:hsa-miR-218-2-3p
  • TargetScanVert:hsa-miR-218-2-3p
  • miRDB:hsa-miR-218-2-3p
  • microrna.org:hsa-miR-218-2-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).