Stem-loop sequence mmu-mir-674

Accession

MI0004611 (change log)

Symbol

MGI:Mir674

Description

Mus musculus miR-674 stem-loop

Gene family

MIPF0000394; mir-674

Literature search

10 open access papers mention mmu-mir-674
(67 sentences)

Stem-loop

      cu  u  u     u        ca             g     ag  u 
5' ggc  ag ca caccc gagccuug  cugagaugggagu gugua  gc c
   |||  || || ||||| ||||||||  ||||||||||||| |||||  ||  
3' ucg  uc gu guggg cucggaac  gacucuacccucg cacgu  ug a
      ac  -  -     -        aa             a     -a  g 

Deep sequencing

48963 reads, 103 reads per million, 108 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Landgraf et al. show that the 5' miRNA product is the predominant one [4].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr2: 117185127-117185226 [+]
• Overlapping transcripts:

  intergenic

Database links

• EntrezGene:Mir674
• MGI:Mir674
• RNAcentral:URS00006DF07F_10090
• miRBase Tracker:MI0004611

Mature sequence mmu-miR-674-5p

• Accession: MIMAT0003740
• Previous IDs: mmu-miR-674-5p;mmu-miR-674
• Sequence: 25 - gcacugagaugggaguggugua - 46
• Deep sequencing: 30637 reads, 104 experiments
• Evidence: experimental; MPSS [1], cloned [2,4], miRAP-cloned [3], Illumina [5-6]
• Database links:
  • RNAcentral:URS0000545517_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-674-5p
  • miRDB:mmu-miR-674-5p
  • microrna.org:mmu-miR-674-5p

Mature sequence mmu-miR-674-3p

• Accession: MIMAT0003741
• Previous IDs: mmu-miR-674-3p;mmu-miR-674*
• Sequence: 60 - cacagcucccaucucagaacaa - 81
• Deep sequencing: 18147 reads, 106 experiments
• Evidence: experimental; MPSS [1], cloned [2,4], Illumina [5-6]
• Database links:
  • RNAcentral:URS00002B277E_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-674-3p
  • miRDB:mmu-miR-674-3p
  • microrna.org:mmu-miR-674-3p

References

1. PMID:16582102 "The expression profile of microRNAs in mouse embryos" Mineno J, Okamoto S, Ando T, Sato M, Chono H, Izu H, Takayama M, Asada K, Mirochnitchenko O, Inouye M, Kato I Nucleic Acids Res. 34:1765-1771(2006).
2. PMID:16954537 "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis" Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E Genome Res. 16:1289-1298(2006).
3. PMID:16973894 "Mouse microRNA profiles determined with a new and sensitive cloning method" Takada S, Berezikov E, Yamashita Y, Lagos-Quintana M, Kloosterman WP, Enomoto M, Hatanaka H, Fujiwara S, Watanabe H, Soda M, Choi YL, Plasterk RH, Cuppen E, Mano H Nucleic Acids Res. 34:e115(2006).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).