Stem-loop sequence mmu-mir-224

Accession

MI0000711 (change log)

Symbol

MGI:Mir224

Description

Mus musculus miR-224 stem-loop

Gene family

MIPF0000088; mir-224

Literature search

44 open access papers mention mmu-mir-224
(146 sentences)

Stem-loop

          ua         u   u      a u   ug  uu 
5' gggcuuu  agucacuag ggu ccguuu g aga  gu  g
   |||||||  ||||||||| ||| |||||| | |||  ||   
3' cccgaaa  ucagugauc ccg gguaaa c uuu  ua  u
          ca         -   u      a -   gu  cg 

Deep sequencing

15873 reads, 11.6 reads per million, 86 experiments

Confidence

Annotation confidence: not enough data

Feedback: Do you believe this miRNA is real?

Comments

Mouse mir-224 is predicted [2] based on homology to a cloned miR from human (MI0000301) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chrX: 72261031-72261112 [-]
• Overlapping transcripts:

  sense

  OTTMUST00000043189 ; Gabre-001; intron 6

  ENSMUST00000064780 ; Gabre-001; intron 6

Clustered miRNAs

< 10kb from mmu-mir-224

• mmu-mir-452: chrX: 72262224-72262308 [-]
• mmu-mir-224: chrX: 72261031-72261112 [-]

Database links

• EntrezGene:Mirn224
• MGI:Mir224
• RNAcentral:URS00006A71D5_10090
• miRBase Tracker:MI0000711

Mature sequence mmu-miR-224-5p

• Accession: MIMAT0000671
• Previous IDs: mmu-miR-224
• Sequence: 8 - uaagucacuagugguuccguu - 28
• Deep sequencing: 15692 reads, 86 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00001F7925_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-224-5p
  • miRDB:mmu-miR-224-5p
  • microrna.org:mmu-miR-224-5p

Mature sequence mmu-miR-224-3p

• Accession: MIMAT0017062
• Previous IDs: mmu-miR-224*
• Sequence: 55 - aaauggugcccuagugacuaca - 76
• Deep sequencing: 177 reads, 19 experiments
• Evidence: experimental; Illumina [5]
• Database links:
  • RNAcentral:URS0000236D35_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-224-3p
  • miRDB:mmu-miR-224-3p

References

1. PMID:12554860 "Numerous microRNPs in neuronal cells containing novel microRNAs" Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G RNA. 9:180-186(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).