Stem-loop sequence mmu-mir-221

Accession

MI0000709 (change log)

Symbol

MGI:Mir221

Description

Mus musculus miR-221 stem-loop

Gene family

MIPF0000051; mir-221

Literature search

197 open access papers mention mmu-mir-221
(1425 sentences)

Stem-loop

   a       cugg   a    -  ug   u         auuu    -   u 
5'  uccaggu    ggc ugaa cc  gca acaauguag    cugu guu g
    |||||||    ||| |||| ||  ||| |||||||||    |||| ||| u
3'  aggucca    ucg acuu gg  cgu uguuacauc    gaca cgg u
   a       ----   g    u  gu   c         ----    a   a 

Deep sequencing

1200021 reads, 2.5e+03 reads per million, 108 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Mouse mir-221 is predicted [2] based on homology to a reported miR from human (MI0000298) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chrX: 19146294-19146388 [-]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mmu-mir-221

• mmu-mir-222: chrX: 19146893-19146971 [-]
• mmu-mir-221: chrX: 19146294-19146388 [-]

Database links

• EntrezGene:Mirn221
• MGI:Mir221
• RNAcentral:URS000075A91F_10090
• miRBase Tracker:MI0000709

Mature sequence mmu-miR-221-5p

• Accession: MIMAT0017060
• Previous IDs: mmu-miR-221*
• Sequence: 20 - accuggcauacaauguagauuucugu - 45
• Deep sequencing: 33185 reads, 101 experiments
• Evidence: experimental; 454 [5], Illumina [6]
• Database links:
  • RNAcentral:URS00003713CD_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-221-5p
  • miRDB:mmu-miR-221-5p

Mature sequence mmu-miR-221-3p

• Accession: MIMAT0000669
• Previous IDs: mmu-miR-221
• Sequence: 60 - agcuacauugucugcuggguuuc - 82
• Deep sequencing: 1166826 reads, 107 experiments
• Evidence: experimental; cloned [3], Illumina [4,6]
• Database links:
  • RNAcentral:URS0000170CF4_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-221-3p
  • miRDB:mmu-miR-221-3p
  • microrna.org:mmu-miR-221-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20668074 "Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68" Zhu JY, Strehle M, Frohn A, Kremmer E, Hofig KP, Meister G, Adler H J Virol. 84:10266-10275(2010).
6. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).