Stem-loop sequence mmu-let-7e

Accession

MI0000561 (change log)

Symbol

MGI:Mirlet7e

Description

Mus musculus let-7e stem-loop

Gene family

MIPF0000002; let-7

Literature search

430 open access papers mention mmu-let-7e
(2159 sentences)

Stem-loop

   c    cc  c   cu   g                u  ggaaga ac 
5'  gcgc  cc ggg  gag uaggagguuguauagu ga      c  c
    ||||  || |||  ||| |||||||||||||||| ||      |   
3'  cgcg  gg ccc  uuc auccuccggcauauca cu      g  c
   c    uc  a   cu   g                -  --agag ag 

Deep sequencing

51253163 reads, 9.65e+04 reads per million, 109 experiments

Confidence

Annotation confidence: high

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Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr17: 17830352-17830444 [+]
• Overlapping transcripts:

  antisense

  ENSMUST00000175058 ; Gm25927-201; exon 1

Clustered miRNAs

< 10kb from mmu-let-7e

• mmu-mir-99b: chr17: 17830188-17830257 [+]
• mmu-let-7e: chr17: 17830352-17830444 [+]
• mmu-mir-125a: chr17: 17830812-17830879 [+]

Database links

• EntrezGene:Mirnlet7e
• MGI:Mirlet7e
• RNAcentral:URS000075CDF6_10090
• miRBase Tracker:MI0000561

Mature sequence mmu-let-7e-5p

• Accession: MIMAT0000524
• Previous IDs: mmu-let-7e
• Sequence: 15 - ugagguaggagguuguauaguu - 36
• Deep sequencing: 51249173 reads, 109 experiments
• Evidence: experimental; cloned [1-4], Illumina [5,7]
• Database links:
  • RNAcentral:URS000000B1C9_10090
• Predicted targets:
  • TargetScanVert:mmu-let-7e-5p
  • miRDB:mmu-let-7e-5p
  • microrna.org:mmu-let-7e-5p

Mature sequence mmu-let-7e-3p

• Accession: MIMAT0017016
• Previous IDs: mmu-let-7e*
• Sequence: 60 - cuauacggccuccuagcuuucc - 81
• Deep sequencing: 3863 reads, 77 experiments
• Evidence: experimental; 454 [6], Illumina [7]
• Database links:
  • RNAcentral:URS0000409B45_10090
• Predicted targets:
  • TargetScanVert:mmu-let-7e-3p
  • miRDB:mmu-let-7e-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:15538371 "A pancreatic islet-specific microRNA regulates insulin secretion" Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
3. PMID:16766679 "Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes" Watanabe T, Takeda A, Tsukiyama T, Mise K, Okuno T, Sasaki H, Minami N, Imai H Genes Dev. 20:1732-1743(2006).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6. PMID:20668074 "Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68" Zhu JY, Strehle M, Frohn A, Kremmer E, Hofig KP, Meister G, Adler H J Virol. 84:10266-10275(2010).
7. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).