Stem-loop sequence rno-mir-322-2

AccessionMI0031763 (change log)
DescriptionRattus norvegicus miR-322-2 stem-loop
Gene family MIPF0000164; mir-322
Literature search

15 open access papers mention rno-mir-322-2
(100 sentences)

   ccucgcuga   -       a  ca     aa             g auu 
5'          cuc cgaaggg ug  gcagc  uucauguuuugga u   g
            ||| ||||||| ||  |||||  ||||||||||||| |    
3'          ggg gcuuccc ac  cgucg  aaguacaaaacuu g   c
   ------aaa   u       c  aa     cg             g aac 
Get sequence
Deep sequencing
698483 reads, 431 reads per million, 503 experiments
Confidence Annotation confidence: high
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Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons [1]. The miR-322 locus has an orthologous sequence in human (MI0001446), which expresses an experimentally validated mature miRNA sequence from its 5' arm, named miR-424. The human mir-424 locus does not appear to contain a homolog of the miR-322 sequence. The mouse ortholog (MI0000590) appears able to express both miR-322 and miR-424. Both miR-322 and miR-424 have been experimentally verified in rat. Landgraf et al. show that the 5' product is the predominant one [3]. The 5' product is therefore renamed miR-322 and the 3' product renamed miR-322*.

Genome context
Coordinates (Rnor_6.0; GCA_000001895.4) Overlapping transcripts
chrX: 158584542-158584636 [+]
Clustered miRNAs
< 10kb from rno-mir-322-2
rno-mir-322-2chrX: 158584542-158584636 [+]
rno-mir-503-2chrX: 158584857-158584927 [+]
rno-mir-351-2chrX: 158585587-158585667 [+]
rno-mir-542-3chrX: 158588529-158588607 [+]
rno-mir-450b-2chrX: 158589637-158589705 [+]
Database links

Mature sequence rno-miR-322-5p

Accession MIMAT0001619
Previous IDsrno-miR-424;rno-miR-322

23 - 


 - 44

Get sequence
Deep sequencing1144242 reads, 493 experiments
Evidence experimental; cloned [2-3], SOLiD [4]

Mature sequence rno-miR-322-3p

Accession MIMAT0000547
Previous IDsrno-miR-322;rno-miR-322*

61 - 


 - 80

Get sequence
Deep sequencing252618 reads, 498 experiments
Evidence experimental; cloned [1-2], SOLiD [4]


PMID:14691248 "Identification of many microRNAs that copurify with polyribosomes in mammalian neurons" Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G Proc Natl Acad Sci U S A. 101:360-365(2004).
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
PMID:20403161 "Small RNA expression and strain specificity in the rat" Linsen SE, de Wit E, de Bruijn E, Cuppen E BMC Genomics. 11:249(2010).