miRBase entry: ppe-MIR477b

Stem-loop ppe-MIR477b


Accession
MI0021625
Description
Prunus persica ppe-MIR477b precursor miRNA
Gene family
MIPF0000216; MIR477


Sequence

cuaaggagaagguuccaacuUCCCUCAAGGGCUCCCAAUAUUccauguuaauacuccgauaucaaaaucaaguaugggaauGUUGGGGGCUCUUUUGGGACGuugaagcccuugcuccuugg
((((((((((((((.((((.((((..((((((((((((((((((......(((((..(((......))).))))).))))))))))))))))))..)))).)))).)).)))).))))))))

Structure
        -    -  c    u    UC                  auguua     cc   au 
cuaaggag aagg uu caac UCCC  AAGGGCUCCCAAUAUUcc      auacu  gau  c
|||||||| |||| || |||| ||||  ||||||||||||||||||      |||||  |||   
gguuccuc uucc ga guuG AGGG  UUCUCGGGGGUUGuaagg      uauga  cua  a
        g    c  a    C    UU                  -----g     -a   aa 


Annotation confidence Not enough data
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Comments
Gao et al. identified small RNAs by sequencing in Prunus mume [1]. The reads were mapped to the Prunus persica genome. This entry represents the Prunus persica sequence, but the evidence for mature RNA expression is from Prunus mume.

Genome context
chrG3: 15489969-15490090 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from ppe-MIR477b
Name Accession Chromosome Start End Strand Confidence




Database links

Mature ppe-miR477b-5p

Accession MIMAT0031171
Description Prunus persica ppe-miR477b-5p mature miRNA
Sequence 21 - UCCCUCAAGGGCUCCCAAUAUU - 42
Evidence experimental
Illumina [2]

Mature ppe-miR477b-3p

Accession MIMAT0027288
Description Prunus persica ppe-miR477b-3p mature miRNA
Sequence 82 - GUUGGGGGCUCUUUUGGGACG - 102
Evidence experimental
Illumina [1-2]

References

  1. PubMed ID: 22863067
    High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot
    Gao Z, Shi T, Luo X, Zhang Z, Zhuang W, Wang L
    BMC Genomics (2012) 13:371

  2. PubMed ID: 22909020
    Unique expression, processing regulation, and regulatory network of peach (Prunus persica) miRNAs
    Zhu H, Xia R, Zhao B, An YQ, Dardick CD, Callahan AM, Liu Z
    BMC Plant Biol (2012) 12:149