miRBase entry: aae-mir-12

Stem-loop aae-mir-12


Accession
MI0013462
Description
Aedes aegypti aae-mir-12 precursor miRNA
Gene family
MIPF0000181; mir-12

Literature search
6 open access papers mention aae-mir-12
(83 sentences)

Sequence

21928 reads, 1123 reads per million, 2 experiments
uucggacUGAGUAUUACAUCAGGUACUGGUgucgauauucgaaucaccaacgaaucggguuccuuugauuaacuuacCAGUACUUAUGUUAUGCUCUCUccg
..((((..((((((.((((.(((((((((((((((.((((((.((......)).))))))....)))))......)))))))))))))).))))))..))))

Structure
uu    cU      U    C          ------     ---u      a  ac 
  cgga  GAGUAU ACAU AGGUACUGGU      gucga    auucga uc  c
  ||||  |||||| |||| ||||||||||      |||||    |||||| ||   
  gccU  CUCGUA UGUA UUCAUGACca      uaguu    ugggcu ag  a
--    CU      U    -          uucaau     uccu      a  ca 


Annotation confidence High
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Comments
This miRNA was identified independently in Aedes aegypti [1] and in the closely related Aedes albopictus, for which there was no genome sequence [2]. The latter reads were therefore also mapped to the Aedes aegypti genome [2].

Genome context
supercont1.68: 2720396-2720497 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from aae-mir-12
Name Accession Chromosome Start End Strand Confidence




Database links

Mature aae-miR-12-5p

Accession MIMAT0014253
Description Aedes aegypti aae-miR-12-5p mature miRNA
Sequence 8 - UGAGUAUUACAUCAGGUACUGGU - 30
Evidence experimental
454 [1], Illumina [2]

Mature aae-miR-12-3p

Accession MIMAT0014254
Description Aedes aegypti aae-miR-12-3p mature miRNA
Sequence 78 - CAGUACUUAUGUUAUGCUCUCU - 99
Evidence experimental
Illumina [2]

References

  1. PubMed ID: 19961592
    Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
    Li S, Mead EA, Liang S, Tu Z
    BMC Genomics (2009) 10:581

  2. PubMed ID: 20167119
    Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
    Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
    BMC Genomics (2010) 11:119