Stem-loop mmu-mir-653

Accession

MI0005557

Symbol

MGI: Mir653

Description

Mus musculus mmu-mir-653 precursor miRNA

Gene family

MIPF0000435; mir-653

Literature search

2 open access papers mention mmu-mir-653
(2 sentences)

Sequence

655 reads, 20 reads per million, 32 experiments

cauucuuucaGUGUUGAAACAAUCUCUACUGaaccaagcuccaaagcgagUUCACUGGAGUUUGUUUCAGUauugcaggagugcu
(((((((.((((((((((((((.(((((.(((((...(((....)))..))))).))))).)))))))))))))).)))))))..

Structure

--       u              U     C     caa   c 
  cauucuu caGUGUUGAAACAA CUCUA UGaac   gcu c
  ||||||| |||||||||||||| ||||| |||||   |||  
  gugagga guuaUGACUUUGUU GAGGU ACUUg   cga a
uc       c              U     C     -ag   a 

Annotation confidence High

Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression was independently shown in human and rat [2].

Genome context

chr6: 3721301-3721385 [-]

Clustered miRNAs

1 other miRNA is < 10 kb from mmu-mir-653

Mature mmu-miR-653-5p

• Accession: MIMAT0004943
• Description: Mus musculus mmu-miR-653-5p mature miRNA
• Sequence: 11 - GUGUUGAAACAAUCUCUACUG - 31
• Evidence: experimental ; RAKE [1], 454 [3], Illumina [4]

Mature mmu-miR-653-3p

• Accession: MIMAT0017284
• Description: Mus musculus mmu-miR-653-3p mature miRNA
• Sequence: 51 - UUCACUGGAGUUUGUUUCAGU - 71
• Evidence: experimental ; Illumina [4]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


3. PubMed ID: 16954537
   Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
   "Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E"
   "Genome Res (2006) 16:1289-1298


4. PubMed ID: 17989215
   RNA sequence analysis defines Dicer's role in mouse embryonic stem cells
   "Calabrese JM, Seila AC, Yeo GW, Sharp PA"
   "Proc Natl Acad Sci U S A (2007) 104:18097-18102