Stem-loop sequence mmu-mir-301b

Accession

MI0004122 (change log)

Symbol

MGI:Mir301b

Description

Mus musculus miR-301b stem-loop

Gene family

MIPF0000034; mir-130

Literature search

24 open access papers mention mmu-mir-301b
(56 sentences)

Stem-loop

   u   -cu     c g        c    uagguugc    c  ug    g 
5'  uuc   gcugg u cgggugcu ugac        acua ug  cugu a
    |||   ||||| | |||||||| ||||        |||| ||  |||| g
3'  aag   cgacc g gucuacga acug        uggu ac  gacg a
   g   cuc     a g        a    -----uua    a  gu    a 

Deep sequencing

161781 reads, 828 reads per million, 108 experiments

Confidence

Annotation confidence: high

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Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr16: 17124400-17124496 [-]
• Overlapping transcripts:

  sense

  ENSMUST00000093336 ; 2610318N02Rik-201; intron 1

Clustered miRNAs

< 10kb from mmu-mir-301b

• mmu-mir-301b: chr16: 17124400-17124496 [-]
• mmu-mir-130b: chr16: 17124061-17124142 [-]

Database links

• EntrezGene:Mir301b
• MGI:Mir301b
• RNAcentral:URS000075B83C_10090
• miRBase Tracker:MI0004122

Mature sequence mmu-miR-301b-5p

• Accession: MIMAT0017232
• Previous IDs: mmu-miR-301b*
• Sequence: 20 - gcucugacuagguugcacuacu - 41
• Deep sequencing: 261 reads, 35 experiments
• Evidence: experimental; Illumina [5]
• Database links:
  • RNAcentral:URS00003952AD_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-301b-5p
  • miRDB:mmu-miR-301b-5p

Mature sequence mmu-miR-301b-3p

• Accession: MIMAT0004186
• Previous IDs: mmu-miR-301b
• Sequence: 55 - cagugcaaugguauugucaaagc - 77
• Deep sequencing: 161490 reads, 108 experiments
• Evidence: experimental; miRAP-cloned [1], cloned [2], 454 [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS0000507675_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-301b-3p
  • miRDB:mmu-miR-301b-3p
  • microrna.org:mmu-miR-301b-3p

References

1. PMID:16973894 "Mouse microRNA profiles determined with a new and sensitive cloning method" Takada S, Berezikov E, Yamashita Y, Lagos-Quintana M, Kloosterman WP, Enomoto M, Hatanaka H, Fujiwara S, Watanabe H, Soda M, Choi YL, Plasterk RH, Cuppen E, Mano H Nucleic Acids Res. 34:e115(2006).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:17989215 "RNA sequence analysis defines Dicer's role in mouse embryonic stem cells" Calabrese JM, Seila AC, Yeo GW, Sharp PA Proc Natl Acad Sci U S A. 104:18097-18102(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).