Stem-loop sequence mmu-mir-1224

Accession

MI0004118 (change log)

Symbol

MGI:Mir1224

Description

Mus musculus miR-1224 stem-loop

Gene family

MIPF0000440; mir-1224

Literature search

22 open access papers mention mmu-mir-1224
(54 sentences)

Stem-loop

   g      cu          ------    u   aucau      c 
5'  ugagga  ggggaggugg      aggg agc     uagagc a
    ||||||  ||||||||||      |||| |||     ||||||  
3'  acuccu  cuucuccacc      uccc ucg     gucucg g
   g      cu          ccccuc    -   acucu      a 

Deep sequencing

33867 reads, 109 reads per million, 69 experiments

Confidence

Annotation confidence: not enough data

Feedback: Do you believe this miRNA is real?

Comments

This sequence was identified as a miRNA candidate by Berezikov et al [1], and confirmed later by more extensive cloning [2].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr16: 20604452-20604536 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000064280 ; Vwa5b2-005; intron 1

  OTTMUST00000086876 ; Vwa5b2-004; intron 18

  ENSMUST00000046188 ; Vwa5b2-005; intron 1

  ENSMUST00000159780 ; Vwa5b2-004; intron 18

  ENSMUST00000096197 ; Vwa5b2-201; intron 22

Database links

• EntrezGene:Mir1224
• MGI:Mir1224
• RNAcentral:URS0000628581_10090
• miRBase Tracker:MI0004118

Mature sequence mmu-miR-1224-5p

• Accession: MIMAT0005460
• Previous IDs: mmu-miR-1224
• Sequence: 1 - gugaggacuggggagguggag - 21
• Deep sequencing: 33804 reads, 58 experiments
• Evidence: experimental; RAKE [1], cloned [2], Illumina [3-4]
• Database links:
  • RNAcentral:URS0000224406_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-1224-5p
  • miRDB:mmu-miR-1224-5p
  • microrna.org:mmu-miR-1224-5p

Mature sequence mmu-miR-1224-3p

• Accession: MIMAT0017231
• Previous IDs: mmu-miR-1224*
• Sequence: 65 - ccccaccucuucucuccucag - 85
• Deep sequencing: 49 reads, 16 experiments
• Evidence: experimental; Illumina [4]
• Predicted targets:
  • TargetScanVert:mmu-miR-1224-3p
  • miRDB:mmu-miR-1224-3p

References

1. PMID:16954537 "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis" Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E Genome Res. 16:1289-1298(2006).
2. PMID:17964270 "Mammalian mirtron genes" Berezikov E, Chung WJ, Willis J, Cuppen E, Lai EC Mol Cell. 28:328-336(2007).
3. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
4. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).