miRBase entry: rno-mir-20b

Stem-loop rno-mir-20b


Accession
MI0003554
Description
Rattus norvegicus rno-mir-20b precursor miRNA
Gene family
MIPF0000001; mir-17

Literature search
32 open access papers mention rno-mir-20b
(71 sentences)

Sequence

1557 reads, 38 reads per million, 264 experiments
guagugcCAAAGUGCUCAUAGUGCAGGUAGguuuugcugcacucuACUGCAGUGUGAGCACUUCUGGuacuc
..((((((((((((((((((.(((((.(((((........)).)))))))).))))))))))).))))))).

Structure
gu       -           G     G   -  uuu 
  agugcCA AAGUGCUCAUA UGCAG UAG gu   g
  ||||||| ||||||||||| ||||| ||| ||    
  ucauGGU UUCACGAGUGU ACGUC Auc ca   c
-c       C           G     -   u  cgu 


Annotation confidence High
Do you think this miRNA is real?
Comments
The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2]. The ends of the miRNA may be offset with respect to previous annotations.

Genome context
chrX: 140117518-140117589 [-]
Clustered miRNAs
4 other miRNAs are < 10 kb from rno-mir-20b
Name Accession Chromosome Start End Strand Confidence




Database links

Mature rno-miR-20b-5p

Accession MIMAT0003211
Description Rattus norvegicus rno-miR-20b-5p mature miRNA
Sequence 8 - CAAAGUGCUCAUAGUGCAGGUAG - 30
Evidence experimental
SOLiD [3]

Mature rno-miR-20b-3p

Accession MIMAT0003212
Description Rattus norvegicus rno-miR-20b-3p mature miRNA
Sequence 46 - ACUGCAGUGUGAGCACUUCUGG - 67
Evidence experimental
cloned [1-2], SOLiD [3]

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 20403161
    Small RNA expression and strain specificity in the rat
    Linsen SE, de Wit E, de Bruijn E, Cuppen E
    BMC Genomics (2010) 11:249

  3. PubMed ID: 16274478
    Identification of clustered microRNAs using an ab initio prediction method
    Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M
    BMC Bioinformatics (2005) 6:267