Stem-loop sequence dre-mir-212

Accession

MI0003385 (change log)

Description

Danio rerio miR-212 stem-loop

Gene family

MIPF0000065; mir-132

Literature search

5 open access papers mention dre-mir-212
(57 sentences)

Stem-loop

        u  a   a  -  u     c       c      c  aa gug 
5' gucag gc uca ua cc uggcu uagacug uuacug ua  u   u
   ||||| || ||| || || ||||| ||||||| |||||| ||  |    
3' cgguc ug agu au gg acuga aucugac aaugac au  a   c
        -  c   c  c  u     c       -      -  ga agu 

Deep sequencing

278 reads, 0 reads per million, 10 experiments

Confidence

Annotation confidence: high

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Comments

This miRNA was predicted by computational methods using conservation between human, mouse and Fugu rubripes sequences [1]. Expression of the excised miR was validated in zebrafish, and the 5' end mapped by PCR. The 3' end was not experimentally determined.

Genome context

• Coordinates (GRCz11; GCA_000002035.4): chr15: 25897446-25897539 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from dre-mir-212

• dre-mir-212: chr15: 25897446-25897539 [+]
• dre-mir-132-1: chr15: 25897715-25897799 [+]

Database links

• EntrezGene:mir212
• EntrezGene:mir212
• miRBase Tracker:MI0003385

Mature sequence dre-miR-212

• Accession: MIMAT0003049
• Sequence: 61 - uaacagucuacagucauggcu - 81
• Deep sequencing: 37 reads, 10 experiments
• Evidence: experimental; PCR [1]
• Predicted targets:
  • TargetScanFish:dre-miR-212

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).