miRBase entry: mml-mir-221

Stem-loop mml-mir-221


Accession
MI0002892
Description
Macaca mulatta mml-mir-221 precursor miRNA
Gene family
MIPF0000051; mir-221


Sequence

43713 reads, 287 reads per million, 9 experiments
ugaacauccaggucuggggcaugaACCUGGCAUACAAUGUAGAUUUCUGUguucguuaggcaacAGCUACAUUGUCUGCUGGGUUUCaggcuaccuggaaacauguucuc
.((((((((((((....(((.(((((((((((.(((((((((....(((((((.....))).))))))))))))).))))))).)))).))))))))))....)))))..

Structure
-u     ----       cugg   a    -       U         AUUU    -   c 
  gaaca    uccaggu    ggc ugaA CCUGGCA ACAAUGUAG    CUGU guu g
  |||||    |||||||    ||| |||| ||||||| |||||||||    |||| ||| u
  cuugu    aggucca    ucg aCUU GGGUCGU UGUUACAUC    GAca cgg u
cu     acaa       ----   g    U       C         ----    a   a 


Annotation confidence High
Do you think this miRNA is real?
Comments
Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [2]. The expression of this mature miRNA was validated by Miska et al [1].

Genome context
chrX: 45879461-45879570 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from mml-mir-221
Name Accession Chromosome Start End Strand Confidence




Database links

Mature mml-miR-221-3p

Accession MIMAT0002583
Description Macaca mulatta mml-miR-221-3p mature miRNA
Sequence 65 - AGCUACAUUGUCUGCUGGGUUUC - 87
Evidence experimental
cloned [1], Illumina [3]
Database links
Predicted targets

Mature mml-miR-221-5p

Accession MIMAT0026590
Description Macaca mulatta mml-miR-221-5p mature miRNA
Sequence 25 - ACCUGGCAUACAAUGUAGAUUUCUGU - 50
Evidence experimental
Illumina [3]
Database links
Predicted targets

References

  1. PubMed ID: 15345052
    Microarray analysis of microRNA expression in the developing mammalian brain
    Miska EA, Alvarez-Saavedra E, Townsend M, Yoshii A, Sestan N, Rakic P, Constantine-Paton M, Horvitz HR
    Genome Biol (2004) 5:R68

  2. PubMed ID: 15652478
    Phylogenetic shadowing and computational identification of human microRNA genes
    "Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E"
    "Cell (2005) 120:21-24

  3. PubMed ID: 23034410
    Birth and expression evolution of mammalian microRNA genes
    "Meunier J, Lemoine F, Soumillon M, Liechti A, Weier M, Guschanski K, Hu H, Khaitovich P, Kaessmann H"
    "Genome Res (2013) 23:34-45