miRBase entry: ppy-mir-10a

Stem-loop ppy-mir-10a


Accession
MI0002789
Description
Pongo pygmaeus ppy-mir-10a precursor miRNA
Gene family
MIPF0000033; mir-10


Sequence

gaucugucugucuucuguauaUACCCUGUAGAUCCGAAUUUGUGuaaggaauuuuguggucacaaauucguaucuaggggaauauguaguugacauaaacacuccgcucg
....(((.((((..((((((((.((((.(((((.((((((((((................)))))))))).))))))))).))))))))..))))...))).........

Structure
-----gauc   --c    uu        A    G     C          uaaggaa 
         ugu   uguc  cuguauaU CCCU UAGAU CGAAUUUGUG       u
         |||   ||||  |||||||| |||| ||||| ||||||||||        
         aca   acag  gauguaua gggg aucua gcuuaaacac       u
gcucgccuc   aau    uu        a    -     u          ugguguu 


Annotation confidence Not enough data
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Comments
Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [1]. The expression of the mature miRNA was not validated.

Genome context
NW_002963091.1: 1547447-1547556 [-]

Database links

Mature ppy-miR-10a

Accession MIMAT0002487
Description Pongo pygmaeus ppy-miR-10a mature miRNA
Sequence 22 - UACCCUGUAGAUCCGAAUUUGUG - 44
Evidence experimental
Illumina [2]

References

  1. PubMed ID: 15652478
    Phylogenetic shadowing and computational identification of human microRNA genes
    "Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E"
    "Cell (2005) 120:21-24

  2. PubMed ID: 22453055
    Annotation of primate miRNAs by high throughput sequencing of small RNA libraries
    "Dannemann M, Nickel B, Lizano E, Burbano HA, Kelso J"
    "BMC Genomics (2012) 13:116