Stem-loop sequence mmu-mir-449a

Accession

MI0001649 (change log)

Previous IDs

mmu-mir-449

Symbol

MGI:Mir449a

Description

Mus musculus miR-449a stem-loop

Gene family

MIPF0000133; mir-449

Literature search

72 open access papers mention mmu-mir-449a
(802 sentences)

Stem-loop

   -c      ug    cuu      -    u          uga  au 
5'   ugugug  augg   ggcagu guau guuagcuggu   gu  g
     ||||||  ||||   |||||| |||| ||||||||||   ||   
3'   acacac  uauc   ucguca cgua caaucgacca   cg  u
   ac      gu    cuc      g    -          cgg  ag 

Deep sequencing

45191 reads, 1.18e+03 reads per million, 91 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Xie et al. [1] refer to this sequence by the internal identifier MIR54. The sequence is unrelated to C. elegans mir-54 (MI0000025).

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr13: 113037534-113037624 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000112400 ; Cdc20b-004; intron 2

  OTTMUST00000112401 ; Cdc20b-002; intron 2

  OTTMUST00000112402 ; Cdc20b-005; intron 2

  OTTMUST00000112399 ; Cdc20b-001; intron 2

  OTTMUST00000112403 ; Cdc20b-003; intron 2

  ENSMUST00000180543 ; Cdc20b-004; intron 2

  ENSMUST00000181568 ; Cdc20b-002; intron 2

  ENSMUST00000109244 ; Cdc20b-005; intron 2

  ENSMUST00000181117 ; Cdc20b-001; intron 2

  ENSMUST00000181741 ; Cdc20b-003; intron 2

Clustered miRNAs

< 10kb from mmu-mir-449a

• mmu-mir-449c: chr13: 113035983-113036091 [+]
• mmu-mir-449b: chr13: 113037419-113037498 [+]
• mmu-mir-449a: chr13: 113037534-113037624 [+]

Database links

• EntrezGene:Mir449a
• MGI:Mir449a
• RNAcentral:URS000064C3C7_10090
• miRBase Tracker:MI0001649

Mature sequence mmu-miR-449a-5p

• Accession: MIMAT0001542
• Previous IDs: mmu-miR-449;mmu-miR-449a
• Sequence: 16 - uggcaguguauuguuagcuggu - 37
• Deep sequencing: 45047 reads, 91 experiments
• Evidence: experimental; cloned [2], Illumina [3-4]
• Database links:
  • RNAcentral:URS00001F5B39_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-449a-5p
  • miRDB:mmu-miR-449a-5p
  • microrna.org:mmu-miR-449a-5p

Mature sequence mmu-miR-449a-3p

• Accession: MIMAT0017180
• Previous IDs: mmu-miR-449a*
• Sequence: 56 - cagcuaacaugcgacugcucuc - 77
• Deep sequencing: 137 reads, 30 experiments
• Evidence: experimental; Illumina [4]
• Database links:
  • RNAcentral:URS000029DF45_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-449a-3p
  • miRDB:mmu-miR-449a-3p

References

1. PMID:15735639 "Systematic discovery of regulatory motifs in human promoters and 3' UTRs by comparison of several mammals" Xie X, Lu J, Kulbokas EJ, Golub TR, Mootha V, Lindblad-Toh K, Lander ES, Kellis M Nature. 434:338-345(2005).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
4. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).