miRBase entry: cel-mir-360

Stem-loop cel-mir-360


Accession
MI0000759
Description
Caenorhabditis elegans cel-mir-360 precursor miRNA
Gene family
MIPF0000210; mir-360

Literature search
5 open access papers mention cel-mir-360
(28 sentences)

Sequence

94 reads, 42 reads per million, 12 experiments
cauucuguuaggaagcaucaaugugUUGUGACCGUUGUUACGGUCAauuugcaaauugaaaaaaUGACCGUAAUCCCGUUCACAAuacauuguucguuuucuccaaaggu
...(((...(((((((..(((((((((((((.((..((((((((((.(((..........))).))))))))))..)).)))))))))))))...)))))))....))).

Structure
cau   -guu       -au             C  UU          a   gcaa 
   ucu    aggaagc   caaugugUUGUGA CG  GUUACGGUCA uuu    a
   |||    |||||||   ||||||||||||| ||  |||||||||| |||     
   gga    ucuuuug   guuacauAACACU GC  UAAUGCCAGU aaa    u
--u   aacc       cuu             U  CC          a   aagu 


Annotation confidence Not enough data
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Comments
This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing. The 5' end of the mature sequence ws mapped by PCR, but the 3' ends have not been experimentally determined.

Genome context
chrX: 5919149-5919258 [+]

Database links

Mature cel-miR-360-5p

Accession MIMAT0015121
Description Caenorhabditis elegans cel-miR-360-5p mature miRNA
Sequence 26 - UUGUGACCGUUGUUACGGUCA - 46
Evidence experimental
CLIPseq [3]
Database links

Mature cel-miR-360-3p

Accession MIMAT0000702
Description Caenorhabditis elegans cel-miR-360-3p mature miRNA
Sequence 65 - UGACCGUAAUCCCGUUCACAA - 85
Evidence experimental
cloned [1], PCR [1], Illumina [2], CLIPseq [3]

References

  1. PubMed ID: 19460142
    Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
    Kato M, de Lencastre A, Pincus Z, Slack FJ
    Genome Biol (2009) 10:R54

  2. PubMed ID: 20062054
    Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
    "Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW"
    "Nat Struct Mol Biol (2010) 17:173-179

  3. PubMed ID: 15317971
    Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
    "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
    "RNA (2004) 10:1309-1322