Stem-loop sequence hsa-mir-299

Accession

MI0000744 (change log)

Symbol

HGNC:MIR299

Description

Homo sapiens miR-299 stem-loop

Gene family

MIPF0000186; mir-299

Literature search

55 open access papers mention hsa-mir-299
(217 sentences)

Stem-loop

        a                      uu 
5' aagaa ugguuuaccgucccacauacau  u
   ||||| ||||||||||||||||||||||  g
3' uucuu gccaaaugguaggguguaugua  a
        c                      ua 

Deep sequencing

7589 reads, 14.9 reads per million, 121 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

The sequence from the 5' arm of this miRNA precursor is the predicted human homologue of mouse miR-299, cloned from mouse embryonic stem cells [1,2], later validated in human [4]. Altuvia et al [3] report the cloning of a miRNA sequence from the 3' arm of the same precursor in human.

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr14: 101023794-101023856 [+]
• Overlapping transcripts:

  antisense

  OTTHUMT00000414334 ; BEGAIN-007; intron 1

  OTTHUMT00000414329 ; BEGAIN-001; intron 2

  OTTHUMT00000414331 ; BEGAIN-005; intron 2

  OTTHUMT00000414332 ; BEGAIN-003; intron 2

  OTTHUMT00000414335 ; BEGAIN-006; intron 2

  OTTHUMT00000414337 ; BEGAIN-004; intron 2

  ENST00000557378 ; BEGAIN-007; intron 1

  ENST00000443071 ; BEGAIN-201; intron 1

  ENST00000355173 ; BEGAIN-001; intron 2

  ENST00000553553 ; BEGAIN-005; intron 2

  ENST00000556188 ; BEGAIN-003; intron 2

  ENST00000554140 ; BEGAIN-006; intron 2

  ENST00000554747 ; BEGAIN-004; intron 2

Clustered miRNAs

< 10kb from hsa-mir-299

• hsa-mir-379: chr14: 101022066-101022132 [+]
• hsa-mir-411: chr14: 101023325-101023420 [+]
• hsa-mir-299: chr14: 101023794-101023856 [+]
• hsa-mir-380: chr14: 101025017-101025077 [+]
• hsa-mir-1197: chr14: 101025564-101025651 [+]
• hsa-mir-323a: chr14: 101025732-101025817 [+]
• hsa-mir-758: chr14: 101026020-101026107 [+]
• hsa-mir-329-1: chr14: 101026785-101026864 [+]
• hsa-mir-329-2: chr14: 101027100-101027183 [+]
• hsa-mir-494: chr14: 101029634-101029714 [+]
• hsa-mir-1193: chr14: 101030052-101030129 [+]
• hsa-mir-543: chr14: 101031987-101032064 [+]
• hsa-mir-495: chr14: 101033755-101033836 [+]

Database links

• EntrezGene:MIR299
• HGNC:MIR299
• RNAcentral:URS0000656761_9606
• miRBase Tracker:MI0000744

Mature sequence hsa-miR-299-5p

• Accession: MIMAT0002890
• Sequence: 7 - ugguuuaccgucccacauacau - 28
• Deep sequencing: 5691 reads, 117 experiments
• Evidence: experimental; cloned [4]
• Database links:
  • RNAcentral:URS000017DBB8_9606
• Predicted targets:
  • TargetMiner:hsa-miR-299-5p
  • TargetScanVert:hsa-miR-299-5p
  • miRDB:hsa-miR-299-5p
  • microrna.org:hsa-miR-299-5p

Mature sequence hsa-miR-299-3p

• Accession: MIMAT0000687
• Previous IDs: hsa-miR-299;hsa-miR-299-5p
• Sequence: 39 - uaugugggaugguaaaccgcuu - 60
• Deep sequencing: 1894 reads, 116 experiments
• Evidence: experimental; cloned [3-4]
• Database links:
  • RNAcentral:URS00003B1F5C_9606
• Predicted targets:
  • TargetMiner:hsa-miR-299-3p
  • TargetScanVert:hsa-miR-299-3p
  • miRDB:hsa-miR-299-3p
  • microrna.org:hsa-miR-299-3p

References

1. PMID:12919684 "Embryonic stem cell-specific MicroRNAs" Houbaviy HB, Murray MF, Sharp PA Dev Cell. 5:351-358(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).