Stem-loop sequence mmu-mir-218-2

Accession

MI0000701 (change log)

Symbol

MGI:Mir218-2

Description

Mus musculus miR-218-2 stem-loop

Gene family

MIPF0000026; mir-218

Literature search

71 open access papers mention mmu-mir-218-2
(539 sentences)

Stem-loop

   gac   uu -cc   gg       uuu         cu        gguggaacga  g 
5'    cag  g   gcg  gcuuucc   gugcuugau  aaccaugu          ug a
      |||  |   |||  |||||||   |||||||||  ||||||||          ||  
3'    guc  c   cgc  cgaaagg   cacgaacug  uugguaca          gc a
   -ac   cu ucu   ua       cgc         uc        --------ag  a 

Deep sequencing

160101 reads, 991 reads per million, 82 experiments

Confidence

Annotation confidence: high

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Comments

Mouse miR-218 is predicted [2] based on homology to a reported miR from human (MI0000295) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr11: 35616816-35616925 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000012061 ; Slit3-001; intron 14

  ENSMUST00000069837 ; Slit3-001; intron 14

Database links

• EntrezGene:Mirn218-2
• MGI:Mir218-2
• RNAcentral:URS000075ED64_10090
• miRBase Tracker:MI0000701

Mature sequence mmu-miR-218-5p

• Accession: MIMAT0000663
• Previous IDs: mmu-miR-218
• Sequence: 25 - uugugcuugaucuaaccaugu - 45
• Deep sequencing: 316666 reads, 79 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS000020D84A_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-218-5p
  • miRDB:mmu-miR-218-5p
  • microrna.org:mmu-miR-218-5p

Mature sequence mmu-miR-218-2-3p

• Accession: MIMAT0005444
• Previous IDs: mmu-miR-218-2*
• Sequence: 67 - caugguucugucaagcaccgcg - 88
• Deep sequencing: 1467 reads, 53 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00001F9A0F_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-218-2-3p
  • miRDB:mmu-miR-218-2-3p
  • microrna.org:mmu-miR-218-2-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).