miRBase entry: mmu-mir-218-1

Stem-loop mmu-mir-218-1


Accession
MI0000700
Symbol
MGI: Mir218-1
Description
Mus musculus mmu-mir-218-1 precursor miRNA
Gene family
MIPF0000026; mir-218

Literature search
72 open access papers mention mmu-mir-218-1
(607 sentences)

Sequence

107885 reads, 1446 reads per million, 78 experiments
gugauaauggagcgagauuuucugUUGUGCUUGAUCUAACCAUGUgcuugcgagguaugagaaAAACAUGGUUCCGUCAAGCACCauggaacgucacgcagcuuucuaca
(((..((.(..(((.(((.((((((.(((((((((..((((((((....................))))))))..))))))))).)))))).))).)))..)))..))).

Structure
-   au  u ga   a   u      U         CU        gcuugcgag 
 gug  aa g  gcg gau uucugU GUGCUUGAU  AACCAUGU         g
 |||  || |  ||| ||| |||||| |||||||||  ||||||||          
 cau  uu c  cgc cug aaggua CACGAACUG  UUGGUACA         u
a   cu  - ga   a   c      C         CC        AAaagagua 


Annotation confidence High
Do you think this miRNA is real?
Comments
Mouse miR-218 is predicted [2] based on homology to a reported miR from human (MIR:MI0000294) [1]. Its expression was later verified by cloning [3].

Genome context
chr5: 48223942-48224051 [+]

Database links

Mature mmu-miR-218-5p

Accession MIMAT0000663
Description Mus musculus mmu-miR-218-5p mature miRNA
Sequence 25 - UUGUGCUUGAUCUAACCAUGU - 45
Evidence experimental
cloned [3], Illumina [4-5]
Database links
Predicted targets

Mature mmu-miR-218-1-3p

Accession MIMAT0004665
Description Mus musculus mmu-miR-218-1-3p mature miRNA
Sequence 64 - AAACAUGGUUCCGUCAAGCACC - 85
Evidence experimental
cloned [3], Illumina [4-5]
Database links
Predicted targets

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 12624257
    Vertebrate microRNA genes
    "Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP"
    "Science (2003) 299:1540

  3. PubMed ID: 20215419
    MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
    "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
    "Mol Hum Reprod (2010) 16:463-471

  4. PubMed ID: 20413612
    Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
    "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
    "Genes Dev (2010) 24:992-1009

  5. PubMed ID: 15634332
    New human and mouse microRNA genes found by homology search
    "Weber MJ"
    "FEBS J (2005) 272:59-73