Stem-loop sequence mmu-mir-218-1

Accession

MI0000700 (change log)

Symbol

MGI:Mir218-1

Description

Mus musculus miR-218-1 stem-loop

Gene family

MIPF0000026; mir-218

Literature search

72 open access papers mention mmu-mir-218-1
(598 sentences)

Stem-loop

   gugauaaugga   a   u      u         cu        ---g   gcg 
5'            gcg gau uucugu gugcuugau  aaccaugu    cuu   a
              ||| ||| |||||| |||||||||  ||||||||    |||   g
3'            cgc cug aaggua cacgaacug  uugguaca    gag   g
   acaucuuucga   a   c      c         cc        aaaa   uau 

Deep sequencing

159947 reads, 1.05e+03 reads per million, 78 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Mouse miR-218 is predicted [2] based on homology to a reported miR from human (MI0000294) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr5: 48223942-48224051 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000096636 ; Slit2-013; intron 1

  OTTMUST00000096653 ; Slit2-016; intron 8

  OTTMUST00000096559 ; Slit2-003; intron 14

  OTTMUST00000096561 ; Slit2-001; intron 14

  OTTMUST00000096560 ; Slit2-002; intron 15

  OTTMUST00000096578 ; Slit2-008; intron 15

  OTTMUST00000096562 ; Slit2-006; intron 16

  OTTMUST00000096577 ; Slit2-007; intron 16

  ENSMUST00000172824 ; Slit2-013; intron 1

  ENSMUST00000174487 ; Slit2-016; intron 8

  ENSMUST00000033967 ; Slit2-003; intron 14

  ENSMUST00000170109 ; Slit2-001; intron 14

  ENSMUST00000174313 ; Slit2-002; intron 15

  ENSMUST00000173107 ; Slit2-008; intron 15

  ENSMUST00000174421 ; Slit2-006; intron 16

  ENSMUST00000173702 ; Slit2-007; intron 16

Database links

• EntrezGene:Mirn218-1
• MGI:Mir218-1
• RNAcentral:URS000075AE6F_10090
• miRBase Tracker:MI0000700

Mature sequence mmu-miR-218-5p

• Accession: MIMAT0000663
• Previous IDs: mmu-miR-218
• Sequence: 25 - uugugcuugaucuaaccaugu - 45
• Deep sequencing: 316666 reads, 79 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS000020D84A_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-218-5p
  • miRDB:mmu-miR-218-5p
  • microrna.org:mmu-miR-218-5p

Mature sequence mmu-miR-218-1-3p

• Accession: MIMAT0004665
• Previous IDs: mmu-miR-218-1*
• Sequence: 64 - aaacaugguuccgucaagcacc - 85
• Deep sequencing: 1381 reads, 57 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS000048005B_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-218-1-3p
  • miRDB:mmu-miR-218-1-3p
  • microrna.org:mmu-miR-218-1-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).