Stem-loop sequence mmu-mir-216a

Accession

MI0000699 (change log)

Previous IDs

mmu-mir-216

Symbol

MGI:Mir216a

Description

Mus musculus miR-216a stem-loop

Gene family

MIPF0000054; mir-216

Literature search

24 open access papers mention mmu-mir-216a
(162 sentences)

Stem-loop

   u    u         cu   a        --   uc 
5'  uggu uaaucucag  ggc acugugag  aug  c
    |||| |||||||||  ||| ||||||||  |||  c
3'  aucg auuaggguc  cug ugacacuc  uac  u
   a    u         -u   g        cu   ua 

Deep sequencing

2228 reads, 0 reads per million, 33 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Mouse mir-216 is predicted [2] based on homology to a reported miR from human (MI0000292) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr11: 28757012-28757083 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000011841 ; RP23-298H6.1-001; intron 2

  ENSMUST00000138164 ; Gm12082-001; intron 2

Clustered miRNAs

< 10kb from mmu-mir-216a

• mmu-mir-216a: chr11: 28757012-28757083 [+]
• mmu-mir-217: chr11: 28763728-28763835 [+]

Database links

• EntrezGene:Mirn216a
• MGI:Mir216a
• RNAcentral:URS00007599E4_10090
• miRBase Tracker:MI0000699

Mature sequence mmu-miR-216a-5p

• Accession: MIMAT0000662
• Previous IDs: mmu-miR-216;mmu-miR-216a
• Sequence: 7 - uaaucucagcuggcaacuguga - 28
• Deep sequencing: 1786 reads, 32 experiments
• Evidence: experimental; cloned [3], Illumina [4]
• Database links:
  • RNAcentral:URS0000318E24_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-216a-5p
  • miRDB:mmu-miR-216a-5p
  • microrna.org:mmu-miR-216a-5p

Mature sequence mmu-miR-216a-3p

• Accession: MIMAT0017054
• Previous IDs: mmu-miR-216a*
• Sequence: 47 - cacaguggucucugggauuaug - 68
• Deep sequencing: 442 reads, 16 experiments
• Evidence: experimental; Illumina [4]
• Database links:
  • RNAcentral:URS00000458CF_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-216a-3p
  • miRDB:mmu-miR-216a-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).