miRBase entry: rno-mir-330

Stem-loop rno-mir-330


Accession
MI0000606
Description
Rattus norvegicus rno-mir-330 precursor miRNA
Gene family
MIPF0000200; mir-330

Literature search
14 open access papers mention rno-mir-330
(35 sentences)

Sequence

189120 reads, 236 reads per million, 491 experiments
acccuuuggcgaucucugccUCUCUGGGCCUGUGUCUUAGGCucuucaagaucuaacgaGCAAAGCACAGGGCCUGCAGAGAgguagcgcucugcuc
.......(((((.(.(((((((((((((((((((.(((..((((.............)))).))))))).))))..))))))))))).).)).))).

Structure
acccuuu   -  u u           --    -    U   AG    uucaa 
       ggc ga c cugccUCUCUG  GGCC UGUG CUU  GCuc     g
       ||| || | |||||||||||  |||| |||| |||  ||||     a
       ucg cu g gauggAGAGAC  CCGG ACAC GAA  CGag     u
------c   u  c c           GU    G    -   -A    caauc 


Annotation confidence High
Do you think this miRNA is real?
Comments
The mature miRNA originating from the 3' arm reported in [1] contains an extra 3' A residue, which conflicts with the precursor sequence shown here. Landgraf et al. show that the 5' miRNA is the predominant one [2]. The 3' mature sequence is renamed miR-300* here.

Genome context
chr1: 80092681-80092777 [+]

Database links

Mature rno-miR-330-5p

Accession MIMAT0004641
Description Rattus norvegicus rno-miR-330-5p mature miRNA
Sequence 21 - UCUCUGGGCCUGUGUCUUAGGC - 42
Evidence experimental
cloned [2], SOLiD [3]

Mature rno-miR-330-3p

Accession MIMAT0000568
Description Rattus norvegicus rno-miR-330-3p mature miRNA
Sequence 60 - GCAAAGCACAGGGCCUGCAGAGA - 82
Evidence experimental
cloned [1], SOLiD [3]

References

  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 14691248
    Identification of many microRNAs that copurify with polyribosomes in mammalian neurons
    "Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G"
    "Proc Natl Acad Sci U S A (2004) 101:360-365

  3. PubMed ID: 20403161
    Small RNA expression and strain specificity in the rat
    Linsen SE, de Wit E, de Bruijn E, Cuppen E
    BMC Genomics (2010) 11:249