miRBase entry: dme-mir-279

Stem-loop dme-mir-279


Accession
MI0000363
Description
Drosophila melanogaster dme-mir-279 precursor miRNA
Gene family
MIPF0000184; mir-279

Literature search
16 open access papers mention dme-mir-279
(228 sentences)

Sequence

401005 reads, 14159 reads per million, 49 experiments
ggaauucauacuacuguuuuuAGUGGGUGGGGGUCCAGUGUUUCACAuugauuuucuuaguauuugUGACUAGAUCCACACUCAUUAAuaacgguaguuc
.........(((((((((.((((((((((.(((((.((....(((((...(((.....)))...))))))).))))).)))))))))).)))))))))..

Structure
ggaauucau         u          G     C  UGUU     uug   u 
         acuacuguu uuAGUGGGUG GGGUC AG    UCACA   auu u
         ||||||||| |||||||||| ||||| ||    |||||   ||| c
         ugauggcaa AAUUACUCAC CCUAG UC    AGUgu   uga u
-------cu         u          A     A  ----     uua   u 


Annotation confidence High
Do you think this miRNA is real?
Comments
miR-279 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the ends of the excised miRNA by cloning.

Genome context
chr3R: 29215585-29215684 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from dme-mir-279
Name Accession Chromosome Start End Strand Confidence




Database links

Mature dme-miR-279-3p

Accession MIMAT0000341
Description Drosophila melanogaster dme-miR-279-3p mature miRNA
Sequence 67 - UGACUAGAUCCACACUCAUUAA - 88
Evidence experimental
Northern [1], cloned [2], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-279-5p

Accession MIMAT0020807
Description Drosophila melanogaster dme-miR-279-5p mature miRNA
Sequence 22 - AGUGGGUGGGGGUCCAGUGUUUCACA - 47
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42