miRBase entry: dme-mir-219

Stem-loop dme-mir-219


Accession
MI0000358
Description
Drosophila melanogaster dme-mir-219 precursor miRNA
Gene family
MIPF0000044; mir-219

Literature search
6 open access papers mention dme-mir-219
(7 sentences)

Sequence

4871 reads, 256 reads per million, 46 experiments
uaauucgauuuuuagcuaUGAUUGUCCAAACGCAAUUCUuguugauauucaauauucaAGGGUUGCGACUGGGCAUCGCGgcucgaaauaagaauacaac
..((((.(((((.((((.((((.(((((..(((((((((((..(((((...))))))))))))))))..))))))))).)))).)))))..)))).....

Structure
---ua    -g     u    a    U     AA           uu     u 
     auuc  auuuu agcu UGAU GUCCA  CGCAAUUCUug  gauau  
     ||||  ||||| |||| |||| |||||  |||||||||||  ||||| c
     uaag  uaaag ucgG GCUA CGGGU  GCGUUGGGAac  uuaua  
caaca    aa     c    C    -     CA           --     a 


Annotation confidence High
Do you think this miRNA is real?
Comments
This sequence is computationally predicted based on conservation in D. pseudoobscura [1]. The sequence of the excised miR is strikingly conserved in human (MIR:MI0000296), but the expression of the miR has not been confirmed in fly and the precise 5' or 3' ends are unknown.

Genome context
chr3L: 17315006-17315105 [+]

Database links

Mature dme-miR-219-5p

Accession MIMAT0000335
Description Drosophila melanogaster dme-miR-219-5p mature miRNA
Sequence 19 - UGAUUGUCCAAACGCAAUUCU - 39
Evidence experimental
454 [2-3], Illumina [3]
Database links
Predicted targets

Mature dme-miR-219-3p

Accession MIMAT0020803
Description Drosophila melanogaster dme-miR-219-3p mature miRNA
Sequence 59 - AGGGUUGCGACUGGGCAUCGCG - 80
Evidence not_experimental
Database links

References

  1. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  2. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  3. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42