miRBase entry: dme-mir-275

Stem-loop dme-mir-275


Accession
MI0000356
Description
Drosophila melanogaster dme-mir-275 precursor miRNA
Gene family
MIPF0000187; mir-275

Literature search
11 open access papers mention dme-mir-275
(19 sentences)

Sequence

187117 reads, 3405 reads per million, 49 experiments
uguaaagucuccuaccuugCGCGCUAAUCAGUGACCGGGGCUgguuuuuuauauacagUCAGGUACCUGAAGUAGCGCGCGugguggcagacauauau
......((((.(((((.(((((((((.((((..(((..(((((..(......)..))))).)))..))))..))))))))).))))).))))......

Structure
uguaaa    c     u         -A    UG   GG     gu uu 
      gucu cuacc ugCGCGCUA  UCAG  ACC  GGCUg  u  u
      |||| ||||| |||||||||  ||||  |||  |||||  |   
      caga ggugg GCGCGCGAU  AGUC  UGG  CUgac  a  u
uauaua    c     u         GA    CA   -A     au ua 


Annotation confidence High
Do you think this miRNA is real?
Comments
miR-275 was reported independently in references [1] and [2]. Computational prediction followed by northern blotting confirmed that the strand containing the predicted miR is predominantly expressed [1]. Reference [2] confirmed the 5' end of the excised miRNA by cloning and reported a length distribution of 19-25 nt with 22 nt the most commonly expressed.

Genome context
chr2L: 7425795-7425892 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from dme-mir-275
Name Accession Chromosome Start End Strand Confidence




Database links

Mature dme-miR-275-3p

Accession MIMAT0000333
Description Drosophila melanogaster dme-miR-275-3p mature miRNA
Sequence 59 - UCAGGUACCUGAAGUAGCGCGCG - 81
Evidence experimental
Northern [1], cloned [2], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-275-5p

Accession MIMAT0020801
Description Drosophila melanogaster dme-miR-275-5p mature miRNA
Sequence 20 - CGCGCUAAUCAGUGACCGGGGCU - 42
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42