Stem-loop sequence hsa-mir-183

Accession

MI0000273 (change log)

Symbol

HGNC:MIR183

Description

Homo sapiens miR-183 stem-loop

Gene family

MIPF0000066; mir-183

Literature search

202 open access papers mention hsa-mir-183
(1025 sentences)

Stem-loop

   ccgcaga   u  ---c  -         g      --ac     ga        --   ac 
5'        gug ga    uc cuguucugu uauggc    uggua  auucacug  uga  a
          ||| ||    || ||||||||| ||||||    |||||  ||||||||  |||   
3'        cac cu    ag gacgagaca auaccg    gccau  uaagugac  acu  g
   -----ag   -  agac  a         a      ggaa     --        ug   cu 

Deep sequencing

281850 reads, 490 reads per million, 153 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and the 5' end mapped by PCR. Expression was later confirmed in human [2,3]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr7: 129774905-129775014 [-]
• Overlapping transcripts:

  antisense

  OTTHUMT00000349347 ; KLHDC10-001; exon 10

  ENST00000335420 ; KLHDC10-001; exon 10

Clustered miRNAs

< 10kb from hsa-mir-183

• hsa-mir-183: chr7: 129774905-129775014 [-]
• hsa-mir-96: chr7: 129774692-129774769 [-]
• hsa-mir-182: chr7: 129770383-129770492 [-]

Database links

• EntrezGene:MIR183
• HGNC:MIR183
• RNAcentral:URS00005BBC98_9606
• miRBase Tracker:MI0000273

Mature sequence hsa-miR-183-5p

• Accession: MIMAT0000261
• Previous IDs: hsa-miR-183
• Sequence: 27 - uauggcacugguagaauucacu - 48
• Deep sequencing: 277729 reads, 151 experiments
• Evidence: experimental; cloned [2-4]
• Database links:
  • RNAcentral:URS0000528CBC_9606
• Predicted targets:
  • TargetMiner:hsa-miR-183-5p
  • TargetScanVert:hsa-miR-183-5p.1
  • miRDB:hsa-miR-183-5p
  • microrna.org:hsa-miR-183-5p

Mature sequence hsa-miR-183-3p

• Accession: MIMAT0004560
• Previous IDs: hsa-miR-183*
• Sequence: 66 - gugaauuaccgaagggccauaa - 87
• Deep sequencing: 4094 reads, 120 experiments
• Evidence: experimental; cloned [3]
• Database links:
  • RNAcentral:URS0000345DEB_9606
• Predicted targets:
  • TargetMiner:hsa-miR-183-3p
  • TargetScanVert:hsa-miR-183-3p
  • miRDB:hsa-miR-183-3p
  • microrna.org:hsa-miR-183-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:12554860 "Numerous microRNPs in neuronal cells containing novel microRNAs" Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G RNA. 9:180-186(2003).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).