sort by

2 publications mentioning ocu-mir-223

Open access articles that are associated with the species Oryctolagus cuniculus and mention the gene name mir-223. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 117
In addition, our results showed that miR-223 was primarily located in the Kupffer cells, but its expression levels were significantly up-regulated in hepatocytes, hepatic stellate cells and Kupffer cells after infection. [score:6]
We found miR-223 was primarily located in the Kupffer cells, and the expression level of miR-223 was significantly up-regulated in all these three types of resident liver cells post-infection, which suggests that the elevated serum miR-223 is derived from the infected liver. [score:6]
Relative miR-223 expression in comparison with uninfected hepatocytes was determined by qPCR (A), and the miR-223 expression levels of hepatocytes, HSCs and Kupffer cells after infection were analyzed (B). [score:5]
Bioinformatics analyses (TargetScan analysis [28] and Gene ontology analysis [29]) revealed a potential role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding (Figure  4C). [score:4]
To identify miRNAs that reflected the schistosome infections and PZQ chemotherapy, six miRNA candidates (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) were selected for analysis in serum that were commonly deregulated in human liver diseases. [score:4]
However, a significant elevated expression level of miR-223 was detected in not only the Kuppfer cells, but also hepatocytes and HSCs after infection (Figure  4B). [score:3]
Therefore, we analyzed the expression level of miR-223 in serum of other hosts of schistosomes, including rabbits, buffalos and humans. [score:3]
Using a mouse mo del of schistosomiasis japonica, we found that the expression level of serum miR-223 was significantly elevated after infection, yet returned to near normal levels after treatment with the anti-helminth drug PZQ. [score:3]
We also conducted a separate experiment to determine the expression level of serum miR-223 in the progression of mouse schistosomiasis. [score:3]
All the rabbits were sacrificed at 56 days after infection to quantify the miR-223 expression level in the sera. [score:3]
We isolated the primary resident livers cells, including hepatocytes, hepatic stellate cells (HSCs) and Kupffer cells, to quantify the expression level of miR-223. [score:3]
Using a mouse mo del of Schistosoma japonicum infection, we found that the expression level of serum miR-223 was significantly elevated after infection, but returned to near normal levels after the treatment with praziquantel (PZQ). [score:3]
Therefore, we analyzed the expression level of miR-223 in serum of other host species of schistosome, including rabbits, buffalos and humans. [score:3]
Figure 4 Expression level of miR-223 in resident liver cells. [score:3]
Expression level of circulating miR-223 in the progression of schistosomiasis. [score:3]
Hydroxyproline content of liver samples (A) and miR-223 expression level of serum samples (B) from the four groups were determined, respectively. [score:3]
Next, we analyzed the expression level of serum miR-223 in the progression of mouse schistosomiasis. [score:3]
Expression levels of serum miR-223 (B), miR-122 (C), miR-34a (D), miR-199a-5p (E) miR-199a-3p (F), and miR-146b (G) were detected in the three groups of mice. [score:3]
Expression level of circulating miR-223 in human and other animal mo dels with schistosomiasis. [score:3]
The expression levels of miR-34a, miR-223, miR-122, miR-146b, miR-199a-5p, miR-199a-3p were determined using the SYBR Green Master Mix kit (TaKaRa, Dalian, China). [score:3]
Figure 2 Correlation between serum miR-223 expression level and liver hydroxyproline content in the infected mice. [score:3]
Considering the Kupffer cells are essential for the development of schistosomiasis -induced inflammation and fibrosis [38], our data implied that miR-223 could play an important role in the pathogenesis and progression of schistosomiasis japonica via regulating the function of Kupffer cells. [score:3]
As shown in Figure  3, the miR-223 expression level was obviously elevated in the serum of infected rabbit, buffalo and human, which validated the results of the miR-223 obtained from the mouse mo del of human schistosomiasis. [score:3]
In this study, we isolated the primary hepatocytes, HSCs and Kupffer cells from the infected and uninfected livers to quantify the expression level of miR-223. [score:3]
It is true that the elevated level of the serum miR-223 was associated with schistosomiasis, but it may not be specifically associated with the disease because the elevated level of the miR-223 was also observed in chronic hepatitis [18]. [score:3]
Figure 3 Expression level of the serum miR-223 in rabbit, buffalo and human with schistosomiasis. [score:3]
Expression level of miR-223 in resident liver cells and its potential function. [score:3]
Importantly, bioinformatics analyses showed that miR-223 potentially functions in transcription regulator activity, transcription factor activity and DNA binding. [score:2]
This suggested that miR-223 could regulate the transcription of some important genes in the Kupffer cells to modulate their function. [score:2]
Bioinformatics analyses revealed a potential functional role of miR-223 in transcription regulator activity, transcription factor activity and DNA binding. [score:2]
Previous studies have also shown that circulating miRNA-146a and miR-223 were significantly reduced in septic patients [19], while serum miRNA-122 and miRNA-192 were elevated in a mouse mo del of drug -induced liver injury [20]. [score:1]
For example, serum miR-21, miR-122 and miR-223 are elevated in patients with hepatocellular carcinoma and chronic hepatitis and thus, have the potential to serve as novel biomarkers for liver injury [18]. [score:1]
In mouse hosts, quantitative PCR result revealed that circulating miR-223, miR-122 and miR-34a were significantly elevated after infection (Figure  1B-D). [score:1]
Only one serum miRNA in infected mice, however, decreased significantly after the PZQ treatment (miR-223, Figure  1B). [score:1]
We validated the elevated level of the circulating miR-223 in serum samples of other host species including rabbits, buffalos and humans. [score:1]
Importantly, the level of serum miR-223 reflected the extent of liver pathology post-infection. [score:1]
In addition, we observed that circulating miR-223 was also significantly elevated in other hosts infected with S. japonicum, including rabbits, buffalos and humans, validating the results of the murine mo del. [score:1]
These results indicate that miR-233 was a schistosomiasis -associated miRNA, and thus circulating miR-223 may serve as a new potential biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy. [score:1]
Bioinformatics analyses were also conducted to assess the potential function of miR-223. [score:1]
Importantly, the level of serum miR-223 was significantly correlated with the hydroxyproline content in the liver tissue (r = 0.808, P < 0.001), which suggests that the level of serum miR-223 could reflect the extent of liver pathology after infection (Figure  2C). [score:1]
This study suggested that the circulating miR-223 could serve as a potential new biomarker for the detection of schistosome infection and the assessment of the response to chemotherapy. [score:1]
As expected, the level of circulating miR-223 increased significantly during the course of infection (Figure  2B). [score:1]
We analyzed the serum levels of six selected candidate miRNA molecules (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) from mice, rabbits, buffalos and humans infected with Schistosoma japonicum using qPCR. [score:1]
Moreover, the serum miR-223 level was significantly correlated with the liver egg burden (r = 0.603, P = 0.008, Figure  2F). [score:1]
For examples, serum miR-155 is the potential biomarker for B-cell lymphoma [30] but also for other cancers such as breast cancer [31], ovarian cancer [32], and pancreatic cancer [33]; Serum miR-92 is the potential biomarker for colon cancer [34], but also leukemia [35], and ovarian cancer [32]; Circulating miR-223 is the potential biomarker for chronic hepatitis [18], but also for schistosomiasis observed in this study. [score:1]
To test this hypothesis, we selected six candidate serum miRNAs for analysis (miR-146b, miR-122, miR-223, miR-199a-5p, miR-199a-3p, miR-34a) in the murine mo del of human schistosomiasis and then performed validation in other host species including rabbits, buffalos and human patients infected with S. japonicum. [score:1]
Importantly, we found that the level of serum miR-223 reflected the extent of liver pathology post-infection. [score:1]
We found that miR-223 was primarily located in the Kuppfer cells of both infected and uninfected livers (Figure  4A). [score:1]
In addition to the association of the serum miR-223 with schistosomiasis, we also showed that the levels of the circulating miR-223 were significantly declined and returned to normality after treatment of the host with the anti-helminth drug, praziquantel. [score:1]
The potential role of miR-223 was analyzed by bioinformatics analyses (C). [score:1]
[1 to 20 of 50 sentences]
2
[+] score: 12
miR-223 is noteworthy because it is frequently suppressed in human HCC [52], and miR-34a, miR-127 and miR-200b are down-regulated in the rat liver during experimental hepatocarcinogenesis [36]. [score:6]
In this study, we found that, in addition to lowered levels of miR-122, expression of miR-223, miR-34a, and miR-127 was also down-regulated. [score:6]
[1 to 20 of 2 sentences]