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10 publications mentioning rno-mir-340-2

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-340-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 88
The relative expression of miRNAs in DCM samples and healthy control samples is shown in Figure 9. We found a 2.6-fold increase in hsa-miR-340 expression (P < 0.001), a 2.4-fold increase in hsa-mir-19b expression (P < 0.01) and a twofold increase in hsa-miR-302 expression (P < 0.05) in DCM samples. [score:9]
To generate a miR-340 expression vector, a ≍260-bp genomic fragment up and downstream of the pre-mir-182 form was amplified by PCR and the fragment containing strictly the pre-miR-340 form was cloned into pGIPZ (Open Biosystems, Pittsburgh, PA, USA) that allows regulated expression of miR-340 upon zeocin treatment. [score:6]
We overexpressed miR-340 in cultured cardiomyocytes to validate its targets. [score:5]
Overexpression of miR-340 significantly elevated the expression levels of ANP, BNP and caspase-3 (P < 0.05; Fig. 11). [score:5]
The cross-sectional area of miR-340 -expressing cells (1952.22 ± 106.59) was significantly (P < 0.0001) greater than that of cells expressing GFP (1059.99 ± 45.59). [score:5]
Hsa-miR-340 regulated 26 target genes including IGF1R and HRB. [score:4]
Importantly, our study identified miRNAs, hsa-miR-19b, hsa-miR-302d and hsa-miR-340 (Fig. 9) which were significantly up-regulated in human DCM and may play a critical role in the pathophysiology of heart failure. [score:4]
In contrast, the miRNAs hsa-miR-340 (14 degrees), hsa-miR-181c (11 degrees) and hsa-miR-182 (10 degrees) were significantly up-regulated in the DCM samples. [score:4]
It remains unclear whether miR-340 is a ‘cause’ or ‘effect’ of the progression of human heart failure; although, it may help to identify key genes in the global signalling networks that are potential targets for miRNAs and play regulatory roles in modulating molecular networks. [score:4]
Fig. 2 Lentiviral (pGIPZ) constructs used to deliver miR-340 expression in cardiomyocytes. [score:3]
The expression levels of miR-340 and the genes ANP, BNP and caspase-3, which are associated with the severity of heart failure and cardiomyocyte apoptosis, were examined by RT-PCR. [score:3]
The relative expression of the genes ANP, BNP and caspase-3 were, respectively, 2.653, 2.000 and 2.095 times greater in miR-340 transfected cells than that control (Fig. 11). [score:3]
Taken together, these data suggested that miR-340 plays a role in regulating nodal molecules in human DCM and that dysregulation of miRNAs may contribute to cardiac dysfunction. [score:3]
The bottom row shows myocardial cells transfected with Lentivirus (only e-GFP, no miR-340), and the upper row shows myocardial cells transfected with Lentivirus (miR-340 overexpression). [score:3]
Studies on miR-340 overexpressed myocardial cells indicated that it may play a pivotal role in the alteration of global signalling networks during cardiac pathogenesis. [score:3]
We found a 5.52-fold increase in miR-340 expression (P < 0.05) in the cultured cardiomyocytes transfected with lentivirus (Fig. 10). [score:3]
Together, these data suggested that miR-340 overexpression is sufficient to induce cardiomyocyte hypertrophy and change cell structure. [score:3]
Furthermore, in vitro study revealed that myocardial cell structure and volume were significantly altered by miR-340 overexpression. [score:3]
There is a 5.52-fold increase in miR-340 expression in the cultured cardiomyocytes transfected with lentivirus than that in the controls (* P < 0.05; ** P > 0.05 versus control). [score:3]
Moreover, miR-340 overexpressed cells showed increased cross-sectional area (P < 0.05) compared to the control group. [score:2]
Fig. 10Quantitative RT-PCR for miR-340. [score:1]
One of the five miRNAs, miR-340, had not previously been identified in end-stage heart failure. [score:1]
The cardiomyocytes were transfected with lentiviral vector–encoded miR-340. [score:1]
Then, e-GFP alone or e-GFP followed by miR-340 (SunBio Medical Biotechnology Company, Shanghai, China) was added to the medium and the cultures were incubated for the indicated time. [score:1]
In the network, the performance differences in the most critical miRNAs (hsa-miR-340, hsa-miR-19a, hsa-miR-19b, etc. ) [score:1]
Importantly, miR-340 was the most significantly altered. [score:1]
The miRNAs hsa-miR-200b (16 degrees), hsa-miR-181c (14 degrees), hsa-miR-340 (13 degrees), hsa-miR-557 (13 degrees), hsa-miR-19a (12 degrees), hsa-miR-19b (12 degrees) and hsa-miR-548f (12 degrees) were significantly differentially regulated in DCM samples compared with non-failing control samples. [score:1]
Real-time PCR demonstrated that hsa-miR-19b, hsa-miR-302d and hsa-miR-340 were significantly increased (P < 0.05), which validate the results from the and implied an important role of miR-340. [score:1]
Fig. 12 Laser confocal microscopy of myocardial cells transfected with e-GFP vector or Lentivirus encoding miR-340. [score:1]
Selected miRNAs (hsa-miR-10a, miR-19b, miR-181c, miR-302d and miR-340) were further quantified with TaqMan qRT-PCR. [score:1]
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2
[+] score: 65
Other miRNAs from this paper: rno-mir-340-1, rno-let-7b, rno-mir-142, rno-mir-144, rno-mir-153
TBI -induced up-regulation of miR-144, miR-153 and miR-340-5p were further confirmed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), and three of their target proteins, calcium/calmodulin -dependent serine protein kinase (CASK), nuclear factor erythroid 2-related factor 2 (NRF2) and alpha-synuclein (SNCA) were found to be concurrently suppressed. [score:8]
In consideration of their correlations with craniocerebral diseases in literature, as well as the involvement of their target genes in cellular component of synaptosome, we would hypothesize that miRNA-miRNA interregulation/interaction among miR-144, miR-153 and miR-340-5p play important roles in the pathophysiological progress of brain injury, especially the development of cognitive dysfunction. [score:7]
While Nfe212 and Gdf10 are putative targets of miR-144 and miR-153, Crot and Plekha3 are potentially regulated by miR-153 and miR-340-5p, which compelled us to validate the expression of these three miRNAs by miRNA-specific qRT-PCR. [score:6]
In addition, protein levels of CASK, NRF2 and SNCA, which are putative targets of miR-144, miR-153 and miR-340-5p, were found to be consistently suppressed in hippocampus after TBI. [score:5]
A large number of target genes involved in enriched GO terms were targeted by miR-144 and miR-340-5p. [score:5]
In addition to miR-144 and miR-153, miR-340-5p was consistently up-regulated in hippocampus post TBI. [score:4]
Our findings suggest that miRNAs, especially miR-144, miR-153 and miR-340-5p, are important mediators in pathophysiological processes after TBI and might serve as potential targets for intervention against brain damage after TBI. [score:3]
Further analysis showed that miR-144 and miR-340-5p share a common target gene Etfdh. [score:3]
Our data suggest that miR-144, miR-153 and miR-340-5p could be potential targets for diagnostic and therapeutic purposes against TBI. [score:3]
qRT-PCR analysis further confirmed that elevated expression of miR-144, miR-153 and miR-340-5p was rapid and long-lasting. [score:3]
GO analysis showed that SH3KBP1, a target of miR-340-5p, is involved in cellular pathway of synaptosome, suggesting that miR-340-5p might also play a role in the pathophysiological process of TBI and TBI -induced cognitive functions. [score:3]
Interestingly, we found strong positive correlations among the expression patterns of miR-144, miR-153 and miR-340-5p in hippocampus after TBI. [score:3]
miR-340-5p is a widespread miRNA and is expressed in various organs of the body [57]– [60]. [score:3]
Expression levels of miR-144 (A), miR-153 (B) and miR-340-5p (C) assessed by miRNA array and miRNA-specific qRT-PCR. [score:3]
miR-144 and miR-340-5p are located in chromosome 10, whereas miR-153 is found in chromosome 6. There is no significant similarity found among these 3 miRNA sequences. [score:1]
However, research into miR-340-5p is still in its infancy, further studies are warranted. [score:1]
Correlation analyses between miR-144 and miR-153 (A), miR-153 and miR-340-5p (B) or miR-144 and miR-340-5p (C). [score:1]
The correlation analyses showed that miR-144, miR-153 and miR-340-5p have positive interrelated relationships between each other (Figure 6), among which the highest correlation coefficient was obtained between miR-153 and miR-340-5p (R [2] = 0.825, P<0.001) (Figure 6B). [score:1]
Correlation between miR-144, miR-153 and miR-340-5p. [score:1]
There is a very strong correlation between miRNA array and qRT-PCR results for miR-144 (R [2] = 0.984, P<0.001), miR-153 (R [2] = 0.982, P<0.001) and miR-340-5p (R [2] = 0.958, P<0.001). [score:1]
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3
[+] score: 17
The miRNA array data have been deposited in the Gene Expression Omnibus (accession number [GEO: GSE22181]) miRNA expression was quantified using real-time RT-PCR on the Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems) to verify the upregulated miRNA targets detected by the miRNA array from the spinal segments (miR-384-3p, miR-325-5p, miR-342-5p, and miR-340-5p) and DRGs (miR-21) in the denervation and sham control groups, and the muscle-specific miRNAs (miR-1, miR-133a, and miR-206) in the soleus muscles of the sham control, entrapment, and decompression groups. [score:10]
In the denervation group, 4 miRNAs (miR-384-3p, miR-325-5p, miR-342-5p, and miR-340-5p) were significantly upregulated, but no miRNA was downregulated in the spinal segment specimens after 6 months. [score:7]
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4
[+] score: 13
A comparison between these identified microRNAs and those showing expression changes in the present microarray analysis revealed similar expression changes for miR-21 24 hours after SCI as well as for 4 other microRNAs, namely, miR-184, miR-340-5p, miR-369-3p and miR-466b, at 7 dpo (Table 2). [score:5]
We observed downregulation of microRNAs related to cell cycle genes such as cdc42 (miR-LET7g) and ctnnb1 (miR340, miR-331), which is consistent with many cell cycle-related genes that have been implicated in neuronal damage after spinal cord injury [8]. [score:4]
These analyses identified four microRNAs with expression changes at 7 dpo; these included miR-340-5p and miR-369-3p, which are both involved in proliferation and adipogenic differentiation, as well as the proapoptotic miR-184 [109] and miR-466b. [score:3]
Specifically, BDNF is related to miR-183, semaphorin 3 to miR-329 and miR-331, Neuritin-1/ nrn1 to miR-331 and miR-342 and Ephrin-B2/ efnb2 to miR-340 and miR-294. [score:1]
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5
[+] score: 9
In contrast to miR-532, miR-340 was only upregulated in the peripheral blood of active UC patients; no changes were observed in the peripheral blood of inactive UC patients [11]. [score:4]
miR-340 was upregulated in the DSS -induced UC colon, suggesting that miR-340 was involved in acute colitis. [score:4]
Five of these miRNAs were altered in colon tissues or peripheral blood in previous studies (miR-126, miR-214, miR-532, miR-140, and miR-340). [score:1]
[1 to 20 of 3 sentences]
6
[+] score: 8
Five differentially expressed microRNAs were randomly selected for validation, including three arterial highly expressed microRNAs (rno-miR-139-3p, rno-miR-423-5p, rno-miR-125b-5p) and two venous highly expressed microRNAs (rno-miR-1-3p, rno-miR-340-3p). [score:7]
For example, seven microRNAs, including miR-1, miR-200c, miR-340, miR-342, miR-325, miR-139 and miR-500 contributed to the association with heart failure (P-value = 2.74e-3). [score:1]
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7
[+] score: 7
It indicated that four miRNAs, including miR-129, miR-18a, miR-206, and miR-340-5p, might regulate the important nerve regeneration -associated gene IGF-1. To verify which one/ones of them were exact regulators, the wild-type and mutant 3′-UTR of IGF-1 sub-cloned into the luciferase reporter vector including single target site mutant (mut 1 and mut 2) and double target site mutant (mut 1&2) were constructed and inserted into the reporter plasmid (Fig.   2a). [score:7]
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8
[+] score: 6
results showed that miR-26a and miR-340-5p were candidate miRNAs for targeting rat TLR3 (Figure 1A). [score:3]
Bioinformatics results showed that miR-26a and miR-340-5p were candidate miRNAs for targeting rat TLR3 (Figure 1A). [score:3]
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9
[+] score: 2
At 24 hours, five miRNAs (rno-miR-214, rno-miR-99a, rno-miR-363*, rno-miR-100 and rno-miR-340–5p) and at 48 hrs 6 miRNAs (rno-miR-34b, rno-miR-500, rno-miR-24-1*, rno-miR-29b, rno-miR-199a-3p, rno-let-7a) showed the most prominent dysregulation (P < 0.001) (Fig.   7B). [score:2]
[1 to 20 of 1 sentences]
10
[+] score: 2
Namely, pregnant rats fed SO and FO diets during the first 12 days of pregnancy showed significant lower expression of miR-449c-5p, miR-134–5p, miR-188, miR-32, miR130a, miR-144–3p, miR-431, miR-142–5p, miR-33, miR-340–5p, miR-301a, miR-30a, miR-106b, and miR-136–5p, as compared with OO, LO, and PO diets. [score:2]
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