sort by

3 publications mentioning oar-mir-411a

Open access articles that are associated with the species Ovis aries and mention the gene name mir-411a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 40
To reveal the role of these miRNAs in the muscular phenotype of myostatin knockout mice, we quantified their expression by qRT-PCR, and observed a significant increase in the expression of miR-411, miR-434-3p, miR-379, and miR-193b in myostatin knockout mice (Figure 1A). [score:7]
Among these, the increased expressions of miR-411, miR-434-3p, and miR-379 were of interest because these miRNAs are expressed from the mouse chromosome 12qF1 (chr12qF1), called as the Dlk1-Dio3 locus (Figure 1B). [score:5]
Quantitative RT-PCR showing the decreased expression of miR-411 (A) miR-540-3p (B) pri-miR-411 (C) and Gtl2 (D) in both wild-type and myostatin knockout mice with age. [score:4]
Figure 5Quantitative RT-PCR showing the decreased expression of miR-411 (A) miR-540-3p (B) pri-miR-411 (C) and Gtl2 (D) in both wild-type and myostatin knockout mice with age. [score:4]
However, the expression of the remaining 9 miRNAs (miR-411, miR-434-3p, miR-299*, miR-193, miR-146b, miR-379, miR-193b, miR-22, and miR-223) has not been verified. [score:3]
Immunofluorescence staining of myotubes with an antibody specific to the myosin heavy chain, which is a terminally differentiated marker of skeletal muscle cells, demonstrated that overexpression of miR-411 and miR-540-3p significantly increased the C2C12 myotube diameter (miR-411; 100 ± 4.89 vs. [score:3]
The expression level of pri-miR-411 also tended to increase (Figure 4A). [score:3]
To determine whether myostatin deficiency activates the transcription of chr12qF1 miRNAs, we further quantified the expression levels of the primary transcripts of miR-127 (pri-miR-127) and miR-411 (pri-miR-411) by qRT-PCR. [score:3]
Figure 1(A) A significant increase in miR-411, miR-434-3p, miR-379, and miR-193b expression in myostatin -deficient skeletal muscle at 13 weeks of age was determined by quantitative RT-PCR. [score:3]
Although we demonstrated the potency of the Dlk1-Dio3 locus derived miRNAs (miR-411 and miR-540-3p) to induce myotube hypertrophy using an in vitro mo del, muscular hypertrophy phenotype observed in the callipyge mutation and myostatin deficiency would be caused by slightly different mechanism. [score:2]
Myotubes were transfected with miR-411, miR-434-5p, and miR-540-3p mimics and immunostained with an anti-myosin heavy chain antibody and DAPI. [score:1]
Transfection of miR-411 and miR-540-3p mimics, but not other miRNAs, significantly increased the myotube diameter. [score:1]
miR-411 and miR-540-3p increase the C2C12 myotube diameters. [score:1]
[1 to 20 of 13 sentences]
2
[+] score: 5
Other miRNAs from this paper: oar-mir-665, oar-mir-1197, oar-mir-3959, oar-mir-134
Additionally, oar-miR-411a-5p and oar-miR-1197-3p were also differentially expressed in Han BB sheep, and they were also found to be abnormally expressed in goat ovaries 22. [score:5]
[1 to 20 of 1 sentences]
3
[+] score: 1
Only five of the sheep miRNAs in our dataset have been deposited into the miRBase database V19: oar-miR-379, oar-miR-127, oar-miR-411a, oar-miR-493, and oar-miR-382. [score:1]
[1 to 20 of 1 sentences]