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5 publications mentioning hsa-mir-3679

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-3679. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 63
Inhibition of miR-3679-3p significantly enhanced 10% matrix mineralization levels at 28-day culture (Inhibitor: 1.64 ± 0.021; control: 1.49 ± 0.056; p value = 0.004). [score:5]
In overall, predicted targets and pathways involving miR-3679-3p belong to cell cycle, differentiation and tissue development, suggesting a dysfunction in bone remo deling in the osteoporotic bone. [score:4]
Consistently with miR-3679-3p overexpression in OP fractured bone, transfection results suggest that miR-3679-3p negatively regulates matrix mineralization by osteoblasts which could lead to an OP phenotype. [score:4]
Two osteoblast-related microRNAs, miR-3679 and miR-4274, were found to be associated with femoral neck BMD and overexpressed in OP fractured bone. [score:3]
Among predicted targets for miR-3679-3p are WHSC1/WHSC1L1, THRAP2, SMAD2, LRP6, SOX4, WNT7A, and MGAT5B. [score:3]
Association between expression levels of mature miRNA miR-3679-3p and miR-4274 with its corresponding own genotype was analyzed. [score:3]
In agreement with these data, an impaired mineralization was observed when miR-3679-3p was overexpressed in osteoblasts. [score:3]
Finally, inhibition of miR-3679-3p in human osteoblastic cells increased matrix mineralization. [score:3]
In our study the AA genotype for both rs6430498 and rs12512664 showed higher expression levels of miR-3679-3p and miR-4274 in hOBs, respectively. [score:3]
MiR-3679 and miR-4274, which harbored BMD -associated SNPs in their pre-miRNA sequence (Fig.   2), were quantified by qPCR in order to compare the expression levels between OP and non-OP bone samples. [score:3]
On the other hand, overexpression of miR-3679-3p in hOBs weakly reduces matrix mineralization although differences were not significant (Mimic: 1.53 ± 0.085; control: 1.68 ± 0.256; p value = 0.589). [score:3]
Figure 3 Correlation between miRNA expression levels and genotypes for miR-3679 and miR-4274. [score:3]
Once cells reached 60–70% of confluence, transient transfections were performed using mirVana mimics and inhibitors of both hsa-miR-3679-3p and hsa-miR-4274. [score:3]
Both miRNAs miR-3679-3p and miR-4274 were significantly overexpressed in the OP samples (Table  3). [score:3]
Moreover, as a sensitivity test, we analyzed the miR-3679-3p and miR-4274 expression levels separated according to the exchanged genotype amongst both miRNAs (rs12512664 and rs6430498 respectively). [score:3]
Cells were transfected with both mimics and inhibitors of miR-3679-3p and miR-4274, and their corresponding controls at day 1, and day 14 after seeding. [score:3]
MiR-3679-5p was not detected in total bone samples or in cultured human osteoblast cells, but miR-3679-3p was present in both sample types. [score:1]
SNP rs6430498 in the miR-3679 was significantly associated with FN BMD in a log-additive (p value = 0.036, Beta coefficient [95% CI] = −0.008 [−0.015 to −0.001] and recessive mo del (p value = 0.021, Beta coefficient [95% CI] = −0.017 [−0.032 to −0.003]); and the SNP rs12512664 in the miR-4274 was also significantly associated with FN BMD in a log-additive (p value = 0.016, Beta coefficient [95% CI] = 0.008 [0.002 to 0.015] and recessive mo del (p value = 0.01, Beta coefficient [95% CI] = 0.015 [0.004 to 0.027). [score:1]
In conclusion, two putative functional SNPs in the pre-miRNA sequence of miR-3679 and miR-4274 were associated with femoral neck BMD. [score:1]
In-vitro assessment of osteoblast activity and matrix mineralizationhOBs were treated with miR-3679-3p and miR-4274 mimics or inhibitors and their respective controls for investigating their role in osteoblast function (Supplemental Fig.   1). [score:1]
Since information about these two miRNAs is very scarce, this study could be considered as a preliminary approach presenting miR-3679-3p and miR-4274 within the complex network of bone regulation. [score:1]
Two mir-SNPs, rs6430498 and rs12512664, within the pre-miRNA sequence of miR-3679 and miR-4234, respectively, were associated with FN BMD in the OSTEOMED2 cohort. [score:1]
Black arrows tag the corresponding allele for rs6430498 in the miR-3679 and rs12512664 in the miR-4274. [score:1]
The miR-3679 is encoded within MGAT5 gene. [score:1]
No significant differences in alkaline phosphatase (ALP) activity and cell proliferation were observed after miR-3679-3p transfection. [score:1]
hOBs were treated with miR-3679-3p and miR-4274 mimics or inhibitors and their respective controls for investigating their role in osteoblast function (Supplemental Fig.   1). [score:1]
Once again, no differences were found in both miRNA analyzed (miR-3679-3p; rs12512664: p-value = 0.713 and miR-4274; rs6430498: p-value = 0.645). [score:1]
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2
[+] score: 7
Six under-expressed microRNAs that were altered at least four fold, including hsa-miR-3646, hsa-miR-17*, hsa-miR-3679-3p, hsa-miR-17, hsa-miR-155, and hsa-miR-146a, (Figure 5A) and ten under-expressed microRNAs that were highly expressed (normalized data ≥6), including hsa-miR-100, hsa-miR-10a, hsa-miR-130b, hsa-miR-146a, hsa-miR-17, hsa-miR-1973, hsa-miR-29a, hsa-miR-31, hsa-miR-31* and hsa-miR-762 (Figure 5B), were selected for further qRT-PCR analyses. [score:7]
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3
[+] score: 6
Other miRNAs from this paper: hsa-mir-940
The authors observed significant down-regulation of miR-3679-5p and up-regulation of miR-940 in the cancer group compared to the other groups (Table 1), suggesting salivary miRNA may potentially be used for early detection of pancreatic cancer. [score:6]
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4
[+] score: 4
Nineteen of the downregulated miRNAs in chronic patients were found to be associated with carcinogenesis in various organs and tissues, such as miR-1207 [40], miR-3162-5p [41, 42], miR-3196 [43, 44], miR-371b-5p [45], miR-574-5p [46, 47], miR-1225-5p [48], miR-4485 [49], miR-572 [50], miR-4299 [51], miR-3679-5p [52], miR-3940-5p [53], miR-638 [54, 55], miR-1202 [56], miR-5787 [57], miR-1973 [58], miR-4532 [59], miR-1275 [60], miR-4728-5p [61], and miR-1915-3p [62]. [score:4]
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5
[+] score: 1
90 Down 3.84E-18 hsa-miR-4448 2.45 Up 1.66E-04 hsa-miR-1250 −5.71 Down 2.05E-169 hsa-miR-4426 2.23 Up 3.53E-05 hsa-miR-139-5p −5.46 Down 3.71E-25 hsa-miR-720 2.08 Up 1.21E-05 hsa-miR-335-5p −5.26 Down 2.00E-41 hsa-miR-4485 1.71 Up 6.14E-08 hsa-miR-362-3p −5.01 Down 3.36E-10 hsa-miR-877-5p 1.68 Up 1.92E-82 hsa-miR-1255a −4.92 Down 1.25E-62 hsa-miR-3679-5p 1.45 Up 5.53E-04 hsa-miR-3622a-5p −4.83 Down 4.30E-09 hsa-miR-21-3p 1.40 Up 7.87E-13 hsa-miR-1293 −4.67 Down 2.87E-08 hsa-miR-2116-3p −4.64 Down 3.67E-14 hsa-miR-365a-3p −4. [score:1]
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