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2 publications mentioning ssc-mir-4331-1

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-4331-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 158
For ssc-miR-4331, it was reported to be detected in porcine intestinal [44], liver [45] and milk [46], and it could be upregulated by transmissible gastroenteritis virus (TGEV) infection and inhibit transcription of TGEV gene 7 via directly targeting cell division cycle -associated protein 7 [47]. [score:9]
In summary, the above results suggested that ssc-miR-204 and ssc-miR-4331 suppressed viral HA and NS expression by directly interacting with them respectively, resulting in inhibiting the replication of SIV-H1N1/2009. [score:8]
In our work, alignment of the binding sites in the viral HA and NS genes for the corresponding seed region of ssc-miR-204 and ssc-miR-4331 was performed among different subtypes (H1N1, H5N1 and H9N2) of influenza A viruses, and results revealed that the sequences of target sites were not conserved, which indicated that ssc-miR-204 or ssc-miR-4331 did not target the corresponding H5N1 and H9N2 viral HA or NS genes to repress their expression. [score:7]
As shown in Figure 3A,B, the ectopic expression of ssc-miR-204 or ssc-miR-4331 markedly suppressed the expression of viral HA1 or NS1, respectively, at mRNA levels. [score:7]
However, the alignment results showed that the sequences of the target sites in viral HA and NS were not conserved across the three different representative subtypes, which indicated that ssc-miR-204 and ssc-miR-4331 might not inhibit the replication of H5N1 or H9N2 influenza A virus by targeting HA and NS respectively. [score:7]
In order to explore swine miRNAs that could regulate the SIV-H1N1/2009 replication, we predicted potential miRNAs targeting the viral genomic RNA by bioinformatics method, and identified ssc-miR-204 and ssc-miR-4331 as negative regulators of SIV-H1N1/2009 replication by targeting viral HA and NS respectively. [score:7]
In order to explore whether ssc-miR-4331 inhibited the H5N1 influenza A virus replication by targeting other viral genes, computational screening for potential miRNAs targeting the whole genome of H5N1 influenza A virus was performed. [score:7]
Referring to the reason why ssc-miR-4331 exhibited the antiviral effect on the replication of H5N1 influenza A virus, it might be due to ssc-miR-4331 targeting and regulating some host genes by as yet unknown mechanisms, for ssc-miR-4331 was predicted not to target other viral genes of H5N1 influenza A virus either. [score:6]
SIV-H1N1/2009 Infection Downregulated the Expression of ssc-miR-204 and ssc-miR-4331. [score:6]
Because of their importance in virus pathogenicity, ssc-miR-204 and ssc-miR-4331 were demonstrated to negatively regulate the replication of SIV-H1N1/2009 by targeting viral HA and NS respectively and repressing their expression levels. [score:6]
As expected, no suppression effect of ssc-miR-204 and ssc-miR-4331 on H9N2 NP expression at mRNA levels (Figure 5D) nor at protein levels (Figure 5F), was observed, which proved that both miRNAs did not have an effect on the replication of H9N2 influenza A virus. [score:5]
Ssc-miR-204 and ssc-miR-4331 Repressed the Expression of Viral HA1 and NS1 Respectively, and Inhibited the Replication of SIV-H1N1/2009. [score:5]
A significant increase of viral HA1 or NS1 mRNA expression was observed when endogenous ssc-miR-204 or ssc-miR-4331 was decreased by treating with the corresponding miRNA inhibitors. [score:5]
In summary, this work screened the genome-wide prediction of swine miRNAs and SIV-H1N1/2009 interactions, and for the first time found that ssc-miR-204 and ssc-miR-4331 negatively regulated the replication of SIV-H1N1/2009 by targeting viral HA and NS respectively, which provided us valuable insight into the involvement of swine miRNAs in the swine influenza viruses infection. [score:4]
Since ssc-miR-204 and ssc-miR-4331 negatively regulated the replication of SIV-H1N1/2009, we wondered whether the virus had an impact on the miRNAs expression. [score:4]
In our work, ssc-miR-204 and ssc-miR-4331 were downregulated by SIV-H1N1/2009 infection. [score:4]
Taken together, these results validated that ssc-miR-204 and ssc-miR-4331 directly interacted with the corresponding HA and NS of SIV-H1N1/2009, and the target sites were located in HA1 and NS1, respectively. [score:4]
ssc-miR-204 and ssc-miR-4331 were validated to target viral HA1 and NS1 of SIV-H1N1/2009 respectively, then the effect of both miRNAs on the expression of HA1 and NS1 were investigated. [score:3]
Therefore, our work was the first to illustrate the expression profile and function of ssc-miR-4331 in response to influenza A virus infection. [score:3]
As expected, the decrease or increase trend of luciferase activity was completely abrogated when NPTr cells were treated with pmirGLO-HA-mut together with ssc-miR-204 mimics or inhibitors (Figure 2C), so was the pair of pmirGLO-NS-mut and ssc-miR-4331 (Figure 2F). [score:3]
However, there were no target sites of ssc-miR-4331 in the eight viral genes of H5N1 influenza A virus (Table 2). [score:3]
However, the relative luciferase activity was only decreased when the cells were cotransfected with ssc-miR-204 and pmirGLO-HA or ssc-miR-4331 and pmirGLO-NS, but remained unchanged when the cells were cotransfected with other pairs of miRNA-genes (Figure 1), which indicated that ssc-miR-204 and ssc-miR-4331 might target viral HA and NS of SIV-H1N1/2009, respectively. [score:3]
In addition, in order to further verify that ssc-miR-204 and ssc-miR-4331 inhibiting the replication of SIV-H1N1/2009 is due to the interaction with HA and NS respectively, future work should focus on generating the HA or NS mutated SIV-H1N1/2009 viruses, in which the binding sites for ssc-miR-204 or ssc-miR-4331 are mutated. [score:3]
These results further indicated the target relationship of ssc-miR-204 and HA, and ssc-miR-4331 and NS. [score:3]
In order to exclude the possibility of ssc-miR-204 and ssc-miR-4331 targeting the luciferase, we cotransfected ssc-miR-204 or ssc-miR-4331 with the luciferase reporter vector pmirGLO into NPTr cells and found that the relative firefly luciferase activities were not changed significantly (Figure 2B,E). [score:3]
The neuraminidase activities of cells supernatants were decreased by ssc-miR-204 from 24 h post-infection, especially, a huge drop was observed at 36 h. Similar results were obtained when cells were treated with ssc-miR-4331 mimics or inhibitors (Figure 4Dā€“F). [score:3]
As shown in Figure 5A,B, the target sites in viral HA and NS for the corresponding seed regions of ssc-miR-204 and ssc-miR-4331 were aligned among H1N1, H5N1 and H9N2 influenza A viruses. [score:3]
Ssc-miR-204 and ssc-miR-4331 Mimics and Inhibitor. [score:3]
NPTr cells overexpressed ssc-miR-204 or ssc-miR-4331 by being transfected with the corresponding miRNA mimics and were infected with SIV-H1N1/2009, then total RNA was extracted and reverse transcribed to cDNA which was subjected to quantitative real-time PCR (qRT-PCR). [score:3]
Validation of ssc-miR-204 and ssc-miR-4331 Targeting Viral HA and NS of SIV-H1N1/2009, Respectively. [score:3]
In conclusion, this result suggested that SIV-H1N1/2009 might weaken the antiviral effect of ssc-miR-204 and ssc-miR-4331 by decreasing their expression levels to facilitate its survival in the host during the infection process. [score:3]
While HA and NS are important parts of viral genome of SIV-H1N1/2009, ssc-miR-204 and ssc-miR-4331 may negatively regulate the virus replication. [score:2]
Therefore, ssc-miR-204 and ssc-miR-4331 might be involved in SIV-H1N1/2009 infection via regulating some host genes. [score:2]
For the first time, ssc-miR-204 and ssc-miR-4331 were verified to target viral HA and NS respectively by dual-luciferase reporter assays. [score:2]
In order to address this speculation, NPTr cells were transfected with ssc-miR-204 or ssc-miR-4331 and infected with H5N1 or H9N2 influenza A virus, then the mRNA and protein levels of viral NP were detected using qRT-PCR and western blotting method, respectively. [score:1]
Furthermore, the binding sites for ssc-miR-204 and ssc-miR-4331 were predicted to be located at nt 575ā€“595 in HA and nt 173ā€“199 in NS respectively (Figure 2A,D). [score:1]
The Effect of ssc-miR-204 and ssc-miR-4331 on the Replication of H5N1 or H9N2 Influenza A Virus. [score:1]
A similar effect was observed after the cells being cotransfected with ssc-miR-4331 and pmirGLO-NS (Figure 2E). [score:1]
Meanwhile, ssc-miR-4331 was detected to significantly decrease the mRNA levels (Figure 5C) and protein levels (Figure 5E) of H5N1 NP unexpectedly. [score:1]
However, few studies revealed ssc-miR-4331 to be involved in influenza A viruses infection to our knowledge. [score:1]
Then, the mRNA levels of ssc-miR-204 and ssc-miR-4331 were subsequently quantified by qRT-PCR method. [score:1]
Secondly, the protein levels of viral HA and NS were not assessed after ssc-miR-204 and ssc-miR-4331 treatment respectively, for we did not obtain the effective antibody for HA and NS of SIV-H1N1/2009 and the commercialized antibodies did not function perhaps because of the sequence specificity of SIV-H1N1/2009. [score:1]
However, this antiviral effects of ssc-miR-204 and ssc-miR-4331 were sequence-specific for SIV-H1N1/2009. [score:1]
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[+] score: 1
A) ssc-miR-215, B) MDM238, C) MDM392, D) ssc-miR-30a, E) ssc-miR-194, F) ssc-miR-374b, G) ssc-miR-155, H) ssc-miR-4331. [score:1]
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