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5 publications mentioning sja-mir-3479

Open access articles that are associated with the species Schistosoma japonicum and mention the gene name mir-3479. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

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[+] score: 25
It is also notable that sja-miR-277 and sja-miR-3479-3p are not the most highly expressed miRNAs in either adult worms or eggs, but are observed at intermediate levels [50]. [score:3]
Moreover, both the serum levels of sja-miR-277 and sja-miR-3479-3p were significantly correlated with hepatic egg burdens (Fig 5E and 5F) and the degree of liver fibrosis (Fig 5G and 5H) in the two mouse strains. [score:1]
However, the serum level of sja-miR-277 showed a stronger correlation with liver fibrosis intensity than that of sja-miR-3479-3p. [score:1]
The serum levels of parasite-derived miRNAs, sja-miR-227 (A) and sja-miR-3479-3p (B) in C57BL/6 mice infected with unisexual male worm(s). [score:1]
However, it does not exclude the fact that adult worms secrete miRNAs into the circulating blood stream, since we have also detected sja-miR-277 and sja-miR-3479-3p in the serum of mice infected with unisexual male worm(s) (S4 Fig). [score:1]
Three of these (sja-bantam, sja-miR-3479-3p, sja-miR-10-5p) were further detected in the plasma of S. japonicum-infected mice by stem-loop RT-PCR analysis [22]. [score:1]
The circulating parasite-specific miRNAs, sja-miR-277 and sja-miR-3479-3p, have potential to be diagnostic markers for schistosomiasis japonica, but the sensitivity for early detection of these miRNAs may not only depend on the parasite load but may also be affected by the host pathology induced by schistosome eggs. [score:1]
The serum levels of sja-miR-277 and sja-miR-3479-3p in C57BL/6 (A and B) and BALB/c (C and D) mice, respectively, during the course of infection. [score:1]
We thus carried out RT-qPCR analysis to determine the dynamic serum levels of five parasite-derived miRNAs (sja-bantam, sja-miR-3479-3p, sja-miR-3096, sja-miR-8185 and sja-miR-277) during S. japonicum infection. [score:1]
Nevertheless, five schistosome-specific miRNAs were identified in the plasma of rabbits infected with S. japonicum using a deep sequencing method and one of them, sja-miR-3479-3p, showed diagnostic potential for S. japonicum infection [22], although further confirmation in other animal mo dels and in patients is required. [score:1]
Detection of parasite-derived miRNAs in the serum of C57BL/6 and BALB/c mice during S. japonicum infectionUsing a deep sequencing method, Cheng et al. identified the presence of five schistosome-specific miRNAs (sja-bantam, sja-miR-3479-3p, sja-miR-10-5p, sja-miR-3096 and sja-miR-8185) in the plasma of S. japonicum-infected rabbits. [score:1]
We also confirmed previous reports of the value of parasite-specific miRNAs (sja-miR-277 and sja-miR-3479-3p) in serum as potential biomarkers for the diagnosis of schistosomiasis japonica. [score:1]
Only two parasite-derived miRNAs (sja-miR-277 and sja-miR-3479-3p) could be reliably detected in serum specimens from both mouse strains (Figs 5A–5D and S3 Fig). [score:1]
More recently, it was shown that S. mansoni-derived miRNAs (miR-277, miR-3479-3p and bantam) in serum could discriminate infected from uninfected individuals [18]. [score:1]
0003965.g005 Fig 5The serum levels of sja-miR-277 and sja-miR-3479-3p in C57BL/6 (A and B) and BALB/c (C and D) mice, respectively, during the course of infection. [score:1]
Using a deep sequencing method, Cheng et al. identified the presence of five schistosome-specific miRNAs (sja-bantam, sja-miR-3479-3p, sja-miR-10-5p, sja-miR-3096 and sja-miR-8185) in the plasma of S. japonicum-infected rabbits. [score:1]
S1 Fig M, Ultra low range DNA ladder; lane 1, ath-miR-159a; lane 2, mmu-miR-122; lane 3, mmu-miR-21; lane 4, mmu-miR-20a; lane 5, mmu-miR-34a; lane 6, ath-miR-159a; lane 7, sja-miR-277; lane 8, sja-miR-3479-3p. [score:1]
In BALB/c mice, sja-miR-277 and sja-miR-3479-3p showed a statistically significant signal over noise as early as 4 and 6 weeks p. i. (Fig 5C and 5D), respectively, while in C57BL/6 mice, these signals were delayed to 6 and 9 weeks p. i. (Fig 5A and 5B), respectively. [score:1]
This was reflected by the differential time phase for detecting sja-miR-277 and sja-miR-3479-3p in serum. [score:1]
M, Ultra low range DNA ladder; lane 1, ath-miR-159a; lane 2, mmu-miR-122; lane 3, mmu-miR-21; lane 4, mmu-miR-20a; lane 5, mmu-miR-34a; lane 6, ath-miR-159a; lane 7, sja-miR-277; lane 8, sja-miR-3479-3p. [score:1]
Further, two circulating parasite-specific miRNAs, sja-miR-277 and sja-miR-3479-3p, were shown to have potential as diagnostic markers for schistosomiasis japonica. [score:1]
Correlations between the serum level of sja-miR-277/sja-miR3479-3p and hepatic egg burden (E and F), as well as the degree of hepatic fibrosis (G and H) were performed for both mouse strains. [score:1]
Three circulating S. mansoni-derived miRNAs, sma-miR-277, sma-miR-3479-3p and sma-bantam, have been shown to have potent diagnostic value in detecting S. mansoni infection [18], but in the current study, only two orthologs, sja-miR-277 and sja-miR-3479-3p, could be reliably detected in the sera of the two mouse strains infected with S. japonicum. [score:1]
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[+] score: 17
Similar results were also observed in cells transfected with miR-8, miR-3479 and miR-1989 mimics and the corresponding recombinant plasmids expressing schistosome mRNA target sites (Fig 3). [score:5]
Among the validated miRNA targets, we found that male enriched miR-8 and miR-3479 regulate the molecules likely involved in the Wnt (GenBank Accession Nos: FN313640 and DQ643829.2) and TGF-β (Accession No. [score:4]
1005423.g003 Fig 3Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
Transfection of miR-2 (A), Let-7a (B), bantam (C), miR-8 (D and E), miR-31 (F), miR-1989 (G), and miR-3479 mimics (H) into HEK293T or Hela cells led to a significant reduction of luciferase activity from co -transfected plasmids containing their corresponding target regions. [score:3]
In S. japonicum, Cai et al demonstrated miR-7-5p, miR-61, miR-219-5p, miR-125a, miR-125b, miR-124-3p, and miR-1 were dominant in males, while bantam, miR-71b-5p, miR-3479-5p and miR-Novel-23-5p were predominantly found in the female parasites [45]. [score:1]
Hela or HEK293T cells were transfected with these recombinant plasmids, control plasmids (pGL3), and the corresponding 2`-O-methyl and phosphorothioate miRNA mimics representing bantam, miR-8, miR-31, miR-1989, miR-3479, or control mimics (scrambled or mismatched seeding region of these miRNA) (S8 Table). [score:1]
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[+] score: 4
Interestingly, bantam and miR-10 were significantly enriched in the libraries of EVs derived from schistosomal adult worms, whereas miR-3479-3p did not appear in those EV libraries [33]. [score:1]
Also, it has been shown that Sja-miR-277 and Sja-miR-3479-3p were detectable in the serum samples of infected mice [41]. [score:1]
Among the 13 known Sja-miRNAs identified in the egg EVs, Sja-bantam, Sja-miR-10 and Sja-miR-3479-3p were all previously detected in serum obtained from rabbits infected with S. japonicum [39]. [score:1]
We found 13 known S. japonicum miRNAs (reads >100) present in the schistosomal egg EV libraries (Table  2 and Additional file 2: Table S1), including three miRNAs (miR-10, bantam and miR-3479-3p) that were present in the plasma of S. japonicum infected host rabbits in a previous study [39]. [score:1]
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[+] score: 3
Individually, sja-let-7, sja-miR-1 sja-miR-7-5p, sja-miR-3479-5p sja-miR-190-5p, sja-miR-71 and sja-miR-71b-5p have the most putative sites within the sex-biased expressed genes (Fig 5C) of which sja-let-7, sja-miR-1 sja-miR-7-5p are male-biased miRNAs, while sja-miR-71b-5p is female-biased [26]. [score:3]
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[+] score: 3
The expression of a set of miRNAs, sja-miR-7-5p, sja-miR-61, sja-miR-219-5p, sja-miR-125a, sja-miR-125b, sja-miR-124-3p and sja-miR-1 were dominant in male worms, while sja-bantam, sja-miR-71b-5p, sja-miR-3479-5p, and sja-Novel-23-5p were predominantly found in the female parasites (Table S6 and S9). [score:3]
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