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4 publications mentioning sja-mir-277

Open access articles that are associated with the species Schistosoma japonicum and mention the gene name mir-277. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 21
It is also notable that sja-miR-277 and sja-miR-3479-3p are not the most highly expressed miRNAs in either adult worms or eggs, but are observed at intermediate levels [50]. [score:3]
We also confirmed previous reports of the value of parasite-specific miRNAs (sja-miR-277 and sja-miR-3479-3p) in serum as potential biomarkers for the diagnosis of schistosomiasis japonica. [score:1]
In BALB/c mice, sja-miR-277 and sja-miR-3479-3p showed a statistically significant signal over noise as early as 4 and 6 weeks p. i. (Fig 5C and 5D), respectively, while in C57BL/6 mice, these signals were delayed to 6 and 9 weeks p. i. (Fig 5A and 5B), respectively. [score:1]
Further, two circulating parasite-specific miRNAs, sja-miR-277 and sja-miR-3479-3p, were shown to have potential as diagnostic markers for schistosomiasis japonica. [score:1]
Moreover, both the serum levels of sja-miR-277 and sja-miR-3479-3p were significantly correlated with hepatic egg burdens (Fig 5E and 5F) and the degree of liver fibrosis (Fig 5G and 5H) in the two mouse strains. [score:1]
Three circulating S. mansoni-derived miRNAs, sma-miR-277, sma-miR-3479-3p and sma-bantam, have been shown to have potent diagnostic value in detecting S. mansoni infection [18], but in the current study, only two orthologs, sja-miR-277 and sja-miR-3479-3p, could be reliably detected in the sera of the two mouse strains infected with S. japonicum. [score:1]
The serum levels of sja-miR-277 and sja-miR-3479-3p in C57BL/6 (A and B) and BALB/c (C and D) mice, respectively, during the course of infection. [score:1]
We thus carried out RT-qPCR analysis to determine the dynamic serum levels of five parasite-derived miRNAs (sja-bantam, sja-miR-3479-3p, sja-miR-3096, sja-miR-8185 and sja-miR-277) during S. japonicum infection. [score:1]
The circulating parasite-specific miRNAs, sja-miR-277 and sja-miR-3479-3p, have potential to be diagnostic markers for schistosomiasis japonica, but the sensitivity for early detection of these miRNAs may not only depend on the parasite load but may also be affected by the host pathology induced by schistosome eggs. [score:1]
However, the serum level of sja-miR-277 showed a stronger correlation with liver fibrosis intensity than that of sja-miR-3479-3p. [score:1]
Only two parasite-derived miRNAs (sja-miR-277 and sja-miR-3479-3p) could be reliably detected in serum specimens from both mouse strains (Figs 5A–5D and S3 Fig). [score:1]
Correlations between the serum level of sja-miR-277/sja-miR3479-3p and hepatic egg burden (E and F), as well as the degree of hepatic fibrosis (G and H) were performed for both mouse strains. [score:1]
0003965.g005 Fig 5The serum levels of sja-miR-277 and sja-miR-3479-3p in C57BL/6 (A and B) and BALB/c (C and D) mice, respectively, during the course of infection. [score:1]
We detected significant increases in the serum level of sja-miR-277 in BALB/c and C57BL/6 mice at 4 and 6 weeks p. i., respectively, earlier than with S. mansoni—8 weeks p. i. -, although a higher challenge was given in their study. [score:1]
More recently, it was shown that S. mansoni-derived miRNAs (miR-277, miR-3479-3p and bantam) in serum could discriminate infected from uninfected individuals [18]. [score:1]
This was reflected by the differential time phase for detecting sja-miR-277 and sja-miR-3479-3p in serum. [score:1]
M, Ultra low range DNA ladder; lane 1, ath-miR-159a; lane 2, mmu-miR-122; lane 3, mmu-miR-21; lane 4, mmu-miR-20a; lane 5, mmu-miR-34a; lane 6, ath-miR-159a; lane 7, sja-miR-277; lane 8, sja-miR-3479-3p. [score:1]
S1 Fig M, Ultra low range DNA ladder; lane 1, ath-miR-159a; lane 2, mmu-miR-122; lane 3, mmu-miR-21; lane 4, mmu-miR-20a; lane 5, mmu-miR-34a; lane 6, ath-miR-159a; lane 7, sja-miR-277; lane 8, sja-miR-3479-3p. [score:1]
However, it does not exclude the fact that adult worms secrete miRNAs into the circulating blood stream, since we have also detected sja-miR-277 and sja-miR-3479-3p in the serum of mice infected with unisexual male worm(s) (S4 Fig). [score:1]
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[+] score: 2
Only one target mRNA for miR-277 failed to be validated in the luciferase assays. [score:2]
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3
[+] score: 2
Membranes were incubated with biotin-labeled probes complementary to candidate miRNA sequences (sja-miR-1a, sja-miR-1b, sja-miR-7, sja-miR-71b, sja-miR-71a, sja-miR-87, sja-miR-124, sja-miR-281, sja-miR-2b, sja-miR-2a, sja-miR-307, sja-miR-31, sja-miR-133, sja-miR-310, sja-miR-8, sja-miR-10, sja-miR-36, sja-miR-61, sja-miR-277, sja-miR-219, sja-miR-190, sja-miR-candidate-03). [score:1]
In some cases (such as sja-miR-10, sja-miR-36, sja-miR-61, sja-miR-133, sja-miR-277, sja-miR-310, and sja-miR-candidate-03), a presumed precursor transcript of about 80 nt was detected by northern blot in addition to the 22 nt species. [score:1]
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4
[+] score: 1
Also, it has been shown that Sja-miR-277 and Sja-miR-3479-3p were detectable in the serum samples of infected mice [41]. [score:1]
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