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11 publications mentioning sja-mir-1

Open access articles that are associated with the species Schistosoma japonicum and mention the gene name mir-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 145
By contrast, in the up-regulated genes of 23DSI, the predicted target genes of miR-1-miR-71-miR-7-miR-7-5p appeared to regulate the ribonucleoprotein complex assembly, cellular protein complex assembly, microtubule -based process, response to oxidative stress, multicellular organismal aging, respiratory electron transport chain, pyrimidine ribonucleoside triphosphate biosynthetic process, positive regulation of epithelial cell differentiation, positive regulation of cell proliferation, apoptosis, energy coupled proton transport, electron transport chain, ATP synthesis-coupled proton transport, anatomical structure formation involved in morphogenesis, ribonucleoprotein complex biogenesis, mitotic cell cycle, larval development, microtubule polymerisation or depolymerisation, female gamete generation, regulation of transcription from RNA polymerase II promoter, and imaginal disc development, among others (Table  2 and Additional file 6: Table S4). [score:12]
In 23 DSI, the high level of bantam and low levels of miR-1, miR-71, miR-7, and miR-7-5p possibly regulated and organised a specific gene expression profile for sexual maturation and egg production by inhibiting and strengthening specific gene expression and metabolic processes. [score:8]
Furthermore, among all samples, bantam was distinctly up-regulated in 23 DSI, and miR-1, miR-71, miR-7-5p, and miR-7 were distinctly up-regulated in 23SSI. [score:7]
To analyse the effect of the differential expression of miRNAs on female development after pairing, we sequenced the libraries of 23DSI and 23SSI, predicted the target genes of miRNA-1-miRNA-71-miRNA-7-miR-7-5p (Additional file 3: Table S1) and bantam (Additional file 4: Table S2), and analysed the differential expression of these genes in 23DSI compared with 23SSI. [score:7]
In unpaired females (23SSI), bantam was notably not up-regulated, whereas miR-1, miR-71, miR-7, and miR-7-5p were significantly up-regulated. [score:7]
Click here for file Predicted target genes of miR-1-miR-71-miR-7-miR-7-5p in up-regulated genes in 23DSI. [score:6]
Although miRNAs do not regulate all genes in organisms, evidence provided by miRNA analyses in the present study indicated that pairing likely limited the expression of non-essential genes through increasing the expression of bantam and specific genes by maintaining miR-1, miR-71, miR-7, and miR-7-5p at relatively low levels. [score:6]
By contrast, in paired females (23DSI), the above mentioned miRNAs were not up-regulated, suggesting that the functions of the target genes of miR-1-miR-71-miR-7-miR-7-5p were required in paired females. [score:6]
Similar miRNA profiles were observed in 18SSI and 18DSI, with the presence of identically expressed high-abundance miRNA, such as miRNA-1, miRNA-71b-5p and let-7. By contrast, in 23DSI and 23SSI, most of these high-abundance miRNAs were down-regulated. [score:6]
Predicted target genes of miR-1-miR-71-miR-7-miR-7-5p in up-regulated genes in 23DSI. [score:6]
We found that the target genes of miR-1-miR-71-miR-7-miR-7-5p, such as ribosomal protein genes (CAX72037.1, CAX71939.1, CAX78482.1, CAX77178.1, AAP06483.1, CAX77387.1, CAX72859.1, CAX70956.1, CAX71543.1, CAX83047.1, CAX70121.1) (Additional file 6: Table S4), thioredoxin peroxidase (CAX75860.1), tubulin (XP_002580033.1, CAX75788.1, CAX75500.1, CAX71989.1, CAX76110.1), ATP synthase- H + transporting (CAX75390.1, CAX76063.1), and cytochrome c oxidase (CAX74747.1, CAX76589.1), among others, were significantly up-regulated. [score:6]
Out of the 50 genes, 33 were the predicted target genes of bantam (Figure  3B), whereas only 2 were predicted target genes of miR-1-miR-71-miR-7-miR-7-5p. [score:5]
revealed that in unpaired females, the highly-expressed miRNA-1, miRNA-71, miRNA-7, and miR-7-5p only inhibited the limited pathways, such as proteasome and ribosome assembly. [score:5]
Differential expression of the predicted target genes of bantam and miRNA-1-miRNA-71-miRNA-7-5p- miR-7 between samples from 23 DSI and 23SSI. [score:5]
For instance, in ribosome assembly, 15 of 49 detected genes in this metabolic process were predicted as the target genes of miR-1-miR-71-miR-7-miR-7-5p, whereas only 1 of 49 genes was the predicted target gene of bantam (Figure  3A). [score:5]
For example, the higher expression of bantam was observed only in 23DSI, whereas higher expression of miR-1, miR-71, miR-7-5p, and miR-7 manifested only in 23SSI (Figure  1B). [score:5]
The predicted target genes of bantam hardly participated in the proteasome, porphyrin metabolism, ribosome, whereas more predicted target genes of miR-1-miR-71-miR-7-miR-7-5p were involved in these process. [score:5]
C. miR-1, with respect to 23DSI, was significantly up-regulated in 23SSI (P < 0.01). [score:4]
Moreover, few of the predicted target genes of miR-1-miR-71-miR-7-miR-7-5p participated in the peroxisome, RNA degradation, mRNA surveillance pathway, axon guidance, basal transcription factors, apoptosis, glycerophospholipid metabolism, insulin signalling pathway, lysosome, regulation of actin cytoskeleton, and endocytosis. [score:4]
Predicted target genes of miR-1-miR-71-miR-7-miR-7-5p in Schistosoma japonicum. [score:3]
The miRNAs with high-abundance, such miRNA-1c, miRNA-1a, miRNA-1, miRNA-71b-5p, let-,7 and so on showed identical expression. [score:3]
Click here for file Predicted target genes of miR-1-miR-71-miR-7-miR-7-5p in Schistosoma japonicum. [score:3]
To confirm the differentially expressed miRNAs in 23DSI, 23SSI, 18DSI, and 18SSI, bantam, miRNA-1, and miR-71 were selected for quantitative RT–PCR analysis. [score:3]
Only several high-abundance miRNAs differentially expressed between 23DSI and 23 SSI, such as bantam, miR-1, miR-71, miR-7, and miR-7-5p. [score:3]
The transcriptomes of 23DSI and 23SSI revealed that the predicted target genes of miRNA-1, miRNA-71, miRNA-7, and miR-7-5p were associated with the ribonucleoprotein complex assembly and microtubule -based process. [score:3]
However, none of the predicted target genes of miR-1-miR-71-miR-7-miR-7-5p are involved the citrate cycle, gastric acid secretion, glycolysis/gluconeogenesis, protein digestion and absorption, aminoacyl-tRNA biosynthesis, fatty acid biosynthesis, and the pentose phosphate pathway. [score:3]
These results suggested that miR-1, miR-71, miR-7, and miR-7-5p played an essential role in regulating ribosomal assembly. [score:2]
Furthermore, the low abundance of miR-1, miR-71, miR-7, and miR-7-5p in 23DSI compared with 23SSI was likely capable of promoting specific gene expression. [score:2]
In particular, various ribosomal protein genes were regulated by miR-1-miR-71-miR-7-miR-7-5p. [score:2]
We found the level of high-abundance miRNAs such as miR-1, miR-1a, miR-1c, miR-71b-5p, and let-7 to be higher in 18DSI and 18SSI than in 23DSI and 23SSI. [score:1]
For example, their levels of miR-1c, miR-1a, miR-1, miRNA-71b-5p, and let-7 were far lower than those in 18 DSI or 18SSI. [score:1]
Similarly, higher amount of miR-71 (Figure  2B) and miR-1 (Figure  2C) were observed in 23SSI than in 23DSI. [score:1]
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2
[+] score: 47
Other miRNAs from this paper: sja-let-7, sja-mir-71a, sja-mir-71b, sja-mir-124
“*”: p < 0.05; “**”: p < 0.001 (Student’s t-test) Table 2 miRNA targets identified by the dual-luciferase expression vector reportor system miRNA Target site ID number Validated miRNA1 T1 T0054493 NO miRNA1 T2 T0198367 YES miRNA1 T3 T0090332 YES miRNA1 T4 T0196767 NO miRNA1 T5 T0023779 YES miRNA1 T6 T0149441 NO had been successfully used in the identification of native Argonaute -associated miRNAs and their target sites in mouse brain previously [23]. [score:9]
“*”: p < 0.05; “**”: p < 0.001 (Student’s t-test) Table 2 miRNA targets identified by the dual-luciferase expression vector reportor system miRNA Target site ID number Validated miRNA1 T1 T0054493 NO miRNA1 T2 T0198367 YES miRNA1 T3 T0090332 YES miRNA1 T4 T0196767 NO miRNA1 T5 T0023779 YES miRNA1 T6 T0149441 NO A growing body of evidence suggests that SjAgo plays an important role in the miRNA pathway [20, 22] and SjAgo1 is the most closely related orthologue to the ancestral Argonaute known to bind miRNA in other organisms based on phylogenetic analysis [31]. [score:7]
Profiling of miRNA1, miRNA21, miRNAlet-7 expression by stem-loop quantitative RT-PCR revealed highly stage-specific expression patterns (see Additional file 8). [score:5]
The mismatch sequence was mutated in the target site which matched to the seed sequence of miRNA-1. Then, miRNA mimics and recombinant plasmids containing potential target sites were co -transfected into HEK293T cells to investigate whether the target site was really interacted with the miRNA. [score:5]
To validate the potential target sites, 6 target sites of miR-1 were randomly selected and cloned into p [mir]GLO vector through MCS located in the 3’UTR of the luciferase gene. [score:5]
Importantly, the expression peaks of miRNA1 appeared in schistosomulum, the stage after infection of the host. [score:3]
b Verification of partial putative target sites of known miRNA1 found by. [score:3]
As shown in Fig.   4 and Table  2, the activity of firefly luciferase was significantly reduced in 3 out of 6 miRNA1 groups, indicating that there are multiple target sites of individual miRNAs. [score:3]
Moreover, a positive rate of 50 % has been achieved in a small-scale verification test of the putative target sites of miRNA1. [score:3]
Moreover, partial target sites of miRNA1 were verified by dual luciferase reportor gene assays. [score:2]
org/), 513 putative known miRNAs which have been identified and released in miRNA-database in all species in previous study were detected, of which 43 conserved miRNAs such as miR-1, miR-21, let-7, etc. [score:1]
Briefly, miRNA duplexes and 2’-O-methyl oligonucleotides mimics (miR-1, miR-21) were chemically synthesized by Genepharma (Shanghai, China) and used for the transfection of HEK293T cells after in-vitro annealing. [score:1]
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3
[+] score: 12
Here, we observed that the expression of a set of miRNAs including sja-bantam, sja-miR-1, sja-miR-124-3p, sja-miR-2a-3p, sja-miR-3492, and sja-miR-36-3p was substantially down-regulated in lung-stage schistosomula compared to cercariae (Table S6), suggesting that the target mRNAs of these miRNAs may encode proteins fulfilling important functions at this stage. [score:7]
The expression of a set of miRNAs, sja-miR-7-5p, sja-miR-61, sja-miR-219-5p, sja-miR-125a, sja-miR-125b, sja-miR-124-3p and sja-miR-1 were dominant in male worms, while sja-bantam, sja-miR-71b-5p, sja-miR-3479-5p, and sja-Novel-23-5p were predominantly found in the female parasites (Table S6 and S9). [score:3]
The expression level of sja-miR-1 was relatively high in male adult worms (1.098±0.228) and female adult worms (0.358±0.021) when compared to other miRNAs, and was not shown in Figure 4A. [score:2]
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4
[+] score: 4
Although sequencing -based miRNAs expression profiling is a tool for measuring the relative abundance of miRNAs, the expression of the miR-1 was not detected by northern blot. [score:3]
Sequence analysis indicated miR-1 as the most abundant miRNA (32 reads). [score:1]
[1 to 20 of 2 sentences]
5
[+] score: 4
Other miRNAs from this paper: sja-let-7, sja-mir-71a, sja-mir-36, sja-mir-71b
In the parasitic nematode Trichinella spiralis, members derived from the miR-1 and let-7 families were predominantly expressed in larvae [45]. [score:3]
Of the miRNAs identified, sja-miR-71b-5p, sja-miR-71, sja-miR-1, sja-miR-36-3p, and sja-124-3p were the most abundant members at the egg stage (Figure 3A). [score:1]
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6
[+] score: 3
Individually, sja-let-7, sja-miR-1 sja-miR-7-5p, sja-miR-3479-5p sja-miR-190-5p, sja-miR-71 and sja-miR-71b-5p have the most putative sites within the sex-biased expressed genes (Fig 5C) of which sja-let-7, sja-miR-1 sja-miR-7-5p are male-biased miRNAs, while sja-miR-71b-5p is female-biased [26]. [score:3]
[1 to 20 of 1 sentences]
7
[+] score: 3
The most highly expressed miRNAs in the parasite, such as sja-miR-71, sja-miR-71b-5p and sja-miR-1 [50], are undetectable in the serum/plasma of animal hosts infected with schistosomes [18, 22]. [score:3]
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8
[+] score: 3
Moreover, it has been shown that five miRNAs (miR-71, miR-71b, miR-1, miR-36, and miR-124) are the most abundant in the egg stage of S. japonicum [34], implying that these miRNAs play important roles in embryo development. [score:2]
In the present study, four of these miRNAs (all except Sja-miR-1) were incorporated into the egg EVs. [score:1]
[1 to 20 of 2 sentences]
9
[+] score: 3
It has been suggested that the dissimilarity of expression profiling for the miR1 and 2 families may be due to the pre-miRNA loop controlling or the result of the different functional roles of mature miRNAs [37]. [score:3]
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10
[+] score: 2
In female worms, the most highly transcribed miRNAs were sha-mir-71a (3.6% of mapped sRNA reads), sha-mir-1 (2.0%), sha-mir-71b (0.7%), sha-mir-125b (0.7%) and sha-bantam (0.3%) (S1 Table). [score:1]
The most highly transcribed miRNAs in male worms were sha-mir-1 (5.9% of mapped sRNA reads), sha-mir-71a (5.8%), sha-mir-125b (1.6%), sha-mir-7a (1.0%) and sha-let-7 (0.6%). [score:1]
[1 to 20 of 2 sentences]
11
[+] score: 2
Considering the critical role that eggs play in the pathogenesis of schistosomiasis, miRNAs in schistosome eggs have also been analyzed and several miRNAs (sja-miR-71b-5p, sja-miR-71, sja-miR-1, sja-miR-36-3p, and sja-124-3p) were shown to be the most abundant in the egg stage [44]. [score:1]
In S. japonicum, Cai et al demonstrated miR-7-5p, miR-61, miR-219-5p, miR-125a, miR-125b, miR-124-3p, and miR-1 were dominant in males, while bantam, miR-71b-5p, miR-3479-5p and miR-Novel-23-5p were predominantly found in the female parasites [45]. [score:1]
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