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10 publications mentioning mmu-mir-1195

Open access articles that are associated with the species Mus musculus and mention the gene name mir-1195. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 77
Expression of Map3k2 and Cyp2s1 was significantly up-regulated by the miR-1195 inhibitor (Figure  5D). [score:8]
We intersected 40 predicted targets of miR-1195 retrieved from TargetScanMouse with 795 genes showing anti-correlated expression to that of miR-1195 (Figure  5A) in Nkx2-1 knockdown cells. [score:8]
A group of 39 miRNAs was significantly down-regulated by Nkx2-1 knock-down including miR-1195 (−4.9 fold), miR-378 (−4.6 fold), miR-449a (−2.1 fold), and miR-130a (−1.9 fold) (Figure  2A and Table  1). [score:5]
By RT-qPCR analysis we showed that absence of phosphorylated NKX2-1 results in a significant increment in the levels of miR-200c and miR-221; miR-1195 is down-regulated although no significantly, showing a similar trend than in Nkx2-1 knockdown experiments in MLE15 cells (Figure  2C). [score:5]
We identified only 4 genes whose changes in expression induced by Nkx2-1 were anti-correlated with changes in miR-1195 expression (Nr1d1, Cyp2s1, Spire1 and Map3k2) (Figure  5B). [score:5]
miR-1195 controls expression of its predicted targets. [score:5]
mmu-miR-200c and mmu-miR-1195 predicted target genes identified in TargetScanMouse 6.2. [score:5]
Because miR-1195 is a mouse specific miRNA no further analyses were performed to determine direct regulation of the identified targets. [score:5]
By miRNA array analyses of mouse epithelial cell lines in which endogenous Nkx2-1 was knocked-down, we revealed that 29 miRNAs were negatively regulated including miR-200c, and 39 miRNAs were positively regulated by Nkx2-1 including miR-1195. [score:4]
Expression patterns of the most altered miRNAs (miR-200c, miR-221, miR-1195, and miR-378) were analyzed in Nkx2-1 knock-down cells by RT-qPCR (Figure  2B) confirming the microarray data. [score:4]
To further evaluate the effect of miR-1195 on predicted downstream genes, we transfected MLE15 cells with a miR-1195 inhibitor to reduce its expression levels. [score:3]
Predicted targets of miR-1195, including the mitogen-activated protein kinase Map3k2 [46] and the extra-hepatic cytochrome P450 enzyme Cyp2s1 [47, 48], responded to changes in miR-1195 levels. [score:3]
Figure 5 Analysis of predicted targets of miR-1195. [score:3]
MLE15 cells were transduced with miRVana miR-1195 inhibitor (4464084 ID MH13628 (Life Technologies) using Lipofectamine RNAiMAX (Life Technologies) protocol. [score:3]
miR-1195, for instance, is positively regulated by Nkx2-1 in the lung epithelial cell system and in normal vs. [score:2]
These data indicate a potential regulatory link between NKX2-1 and miR-200c, miR-221, or miR-1195. [score:2]
For miR-200c, the enrichment of the IP fractions with the Nkx2-1 antibody and IgG were 28 and 0.4% of input while for miR-1195, were 129% and 1.3% of input respectively. [score:1]
miR-221 and miR-1195 5′ flanking regions were not represented in the array. [score:1]
After 48 h of transduction, expression levels of miR-1195 were measured by RT-qPCR confirming that miR-1195 was reduced by 50% (Figure  5D). [score:1]
miR-1195 is a mouse specific miRNA [45], enriched in epithelial structures in the embryo and moderately homologous to the human miRNA hsa-miR-584-3p (miRBase [36]). [score:1]
hsa-miR-584 shares partial homology to mmu-miR-1195 [36] and will be analyzed in future studies. [score:1]
Because miR-1195 is not found in humans, its close homolog hsa-miR-584-3p will be the focus of further studies. [score:1]
After several attempts we were unable to clone the 5′flanking region of miR-1195. [score:1]
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2
[+] score: 8
In response to OxS, the expression levels of miR-21-5p, miR-1195, miR-181a-5p, and miR-191-5p (as shown in Fig.   8) were changed significantly and this could result in the observed increased levels of STAT3. [score:3]
The levels of microRNAs (miR-21-5p, miR-181a-5p, miR-1195) in response to O [3] are shown in Table  1; these are known to interact directly with STAT3. [score:2]
miR-21-5p, miR-181a-5p, and miR-1195 shown in the present study to be altered significantly in response to O [3] have also been shown to directly interact with STAT3. [score:2]
miR-1195 has been shown by in silico analysis to bind STAT3 [62], while miR-181a-5p has been shown with quantitative RT-PCR to be increased after activation of STAT3 by IL-6 [63]. [score:1]
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3
[+] score: 8
The miRNA targets that were significantly dysregulated in the array experiments are shown in Table  1. In the whole blood samples, when compared to those with untreated isograft, there were 5 (miR-720, miR-709, miR-2145, miR-1195, and miR-690) and 4 (let-7d, miR-26a, let-7i, and left-7a) downregulated miRNA targets in the mice receiving allograft without and with FK506 treatment, respectively. [score:8]
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4
[+] score: 7
We identified two upregulated miRNAs, namely miR-494 and miR-695 and six downregulated miRNAs, namely miR-331, miR-467b, miR-467f, miR-1195, miR-101b and miR-690 with statistical significance (p<0.05). [score:7]
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5
[+] score: 6
The top five upregulated miRNAs (miR-1195, miR-223-3p, miR-129-2-3p, miR-709, and miR-224-5p) were selected for subsequent analysis, which was performed via quantitative real-time PCR analysis of the plasma from individual mice in each group. [score:4]
Five miRNAs (miR-1195, miR-223-3p, miR-129-2-3p, miR-709, and miR-224-5p), whose levels were found to be significantly elevated in the pooled plasma of ß-glucan -injected SKG mice by panel real-time PCR, were analyzed. [score:1]
We were unable to detect miR-1195, miR-129-2-3p, and miR-224-5p in the plasma of mice in either group (Fig 3). [score:1]
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6
[+] score: 3
Four of the pre-miRNAs with observed novel miR* had some coverage in the lower depth heart biopsy dataset, and thus we could confirm expression of the novel miR-1944*, miR-3470* and miR-1195* (Dataset S2). [score:3]
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7
[+] score: 3
miRNA p-value Fold-Change miR-146a 1.15E-09 −4.82 miR-31 9.66E-08 −4.95 miR-155 1.97E-07 −3.82 miR-2134 2.13E-07 −2.05 miR-711 1.63E-06 −4.10 miR-3473 1.95E-06 −5.62 miR-574-3p 3.32E-06 −2.55 miR-1195 6.59E-06 2.26 miR-27a* 6.89E-06 14.92 miR-27b* 7.35E-06 2.92 miR-34c 1.80E-05 −3.43 miR-1931 3.34E-05 −2.30 miR-874 4.07E-05 −2.01 miR-196b 7.99E-05 2.59 miR-181a-1* 8.02E-05 4.15 miR-187 8.11E-05 2. 02List of miRs whose expression is altered, identified from microarray analysis by comparison of levels in TM [+] and TM [−] DCs which change >2 fold with p<0.0001. [score:3]
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8
[+] score: 1
However, mmu-miR-709 and mmu-miR-1195 are identical to ribosomal sequences and still valid in the current mirBase release 19. [score:1]
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9
[+] score: 1
Other miRNAs from this paper: hsa-mir-25, hsa-mir-28, hsa-mir-95, mmu-mir-151, mmu-mir-290a, mmu-mir-297a-1, mmu-mir-297a-2, mmu-mir-130b, mmu-mir-340, mmu-mir-25, mmu-mir-28a, hsa-mir-130b, hsa-mir-367, hsa-mir-372, hsa-mir-378a, mmu-mir-378a, hsa-mir-340, hsa-mir-151a, mmu-mir-466a, mmu-mir-467a-1, hsa-mir-505, hsa-mir-506, mmu-mir-367, hsa-mir-92b, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-648, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-659, hsa-mir-421, hsa-mir-151b, hsa-mir-1271, hsa-mir-378d-2, mmu-mir-467b, mmu-mir-297b, mmu-mir-505, mmu-mir-297a-3, mmu-mir-297a-4, mmu-mir-297c, mmu-mir-421, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-467c, mmu-mir-467d, mmu-mir-92b, mmu-mir-466d, hsa-mir-297, mmu-mir-467e, mmu-mir-466l, mmu-mir-669g, mmu-mir-466i, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-467f, mmu-mir-466j, mmu-mir-467g, mmu-mir-467h, hsa-mir-548e, hsa-mir-548j, hsa-mir-1285-1, hsa-mir-1285-2, hsa-mir-1289-1, hsa-mir-1289-2, hsa-mir-548k, hsa-mir-1299, hsa-mir-548l, hsa-mir-1302-1, hsa-mir-1302-2, hsa-mir-1302-3, hsa-mir-1302-4, hsa-mir-1302-5, hsa-mir-1302-6, hsa-mir-1302-7, hsa-mir-1302-8, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-1255a, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-1268a, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-1255b-1, hsa-mir-1255b-2, mmu-mir-1906-1, hsa-mir-1972-1, hsa-mir-548q, mmu-mir-466m, mmu-mir-466o, mmu-mir-467a-2, mmu-mir-467a-3, mmu-mir-466c-2, mmu-mir-467a-4, mmu-mir-466b-4, mmu-mir-467a-5, mmu-mir-466b-5, mmu-mir-467a-6, mmu-mir-466b-6, mmu-mir-467a-7, mmu-mir-466b-7, mmu-mir-467a-8, mmu-mir-467a-9, mmu-mir-467a-10, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, hsa-mir-3116-1, hsa-mir-3116-2, hsa-mir-3118-1, hsa-mir-3118-2, hsa-mir-3118-3, hsa-mir-548s, hsa-mir-378b, hsa-mir-466, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-3156-1, hsa-mir-3118-4, hsa-mir-3174, hsa-mir-3179-1, hsa-mir-3179-2, hsa-mir-3179-3, hsa-mir-548w, hsa-mir-3156-2, hsa-mir-3156-3, hsa-mir-548x, mmu-mir-3470a, mmu-mir-3470b, mmu-mir-3471-1, mmu-mir-3471-2, hsa-mir-378c, hsa-mir-1972-2, hsa-mir-1302-9, hsa-mir-1302-10, hsa-mir-1302-11, mmu-mir-1906-2, hsa-mir-3683, hsa-mir-3690-1, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-1268b, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-378f, hsa-mir-378g, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-378h, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-378i, hsa-mir-548am, hsa-mir-548an, mmu-mir-28c, mmu-mir-378b, mmu-mir-28b, hsa-mir-548ao, hsa-mir-548ap, mmu-mir-466q, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-378j, mmu-mir-378c, mmu-mir-378d, hsa-mir-548ay, hsa-mir-548az, hsa-mir-3690-2, mmu-mir-290b, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-3179-4, mmu-mir-466c-3, hsa-mir-548bc, mmu-mir-1271
We also identified one murine-specific families, mir-1906 family, and some large families, for instance, mir-1195, mir-1937, mir-3470 and mir-3471 families are mouse-specific (Table S2). [score:1]
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10
[+] score: 1
Most candidates had 5′seed sequences distinct from known miRNAs; however the following candidates, conserved with humans, were similar to those in parentheses: 5 (miR-190/190b), 19 (miR-29b-2), 92 (miR-1195), 93 (miR-134), 132 (miR-486), and 183 (miR-345-3p). [score:1]
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