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9 publications mentioning mmu-mir-1187

Open access articles that are associated with the species Mus musculus and mention the gene name mir-1187. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 97
Taken together, we speculate that overexpression of miR-1187 suppresses caspase-8, then down-regulates downstream genes including caspase-3, caspase-7 and caspase-9, consequently attenuating the apoptosis of hepatocytes. [score:8]
We also found miRNAs including miR-155, miR-146a, miR-125a, miR-15b and miR-16 were up-regulated and miR-1187 was down-regulated significantly during ALF in mice (17). [score:7]
After induction, a down-regulation of miR-1187 but an up-regulation of caspase-8 (both mRNA and protein level) was detected. [score:7]
In summary, our study demonstrated that miR-1187 regulated hepatocytes apoptosis via targeting caspase-8. miR-1187 acted as an inhibitor of hepatocyte apoptosis which shed light on the treatment of ALF. [score:6]
Inversely, caspase-8 mRNA was up-regulated in BNLCL2 cells induced with D-GalN/TNF (P<0.05), but overexpression of miR-1187 reduced caspase-8 mRNA significantly (P<0.01, Fig. 2d). [score:6]
Overexpression of miR-1187 suppressed the level of caspase-8 mRNA as well as protein in BNLCL2 cells induced by D-GalN/TNF. [score:5]
miR-1187 was significantly decreased during ALF and was predicted to target the caspase-8 mRNA 3′-untranslated region (3′UTR). [score:5]
Furthermore, using the TargetScan database, it was found that caspase-8 was a putative target of miR-1187. [score:5]
Taken together, it was presumed that miR-1187 regulated caspase-8 by mRNA degradation and the level of protein was suppressed accordingly. [score:4]
Up-regulated miR-1187 attenuated apoptosis of BNLCL2 cells. [score:4]
However, it is still unclear whether down-regulation of miR-1187 plays a role in hepatocyte apoptosis. [score:4]
However, overexpression of miR-1187 attenuated the apoptotic rate significantly, i. e. by about 21, 24, 29 and 36%, respectively (P<0.05, Fig. 4). [score:3]
qRT-PCR was applied to verify the expression of miR-1187, and the result was consistent with the microarray data (P<0.01, Fig. 1g). [score:3]
Moreover, overexpression of miR-1187 reduced the levels of caspase-8 mRNA and protein and attenuated the apoptotic rate as well. [score:3]
Thus, it is reasonable to conclude that miR-1187 contributes to hepatocyte apoptosis via targeting caspase-8 during ALF. [score:3]
Cleaved caspase-8 protein was increased in BNLCL2 cells induced by D-GalN/TNF, but it was significantly suppressed (P<0.05) when the cells were transfected with the miR-1187 mimic (D/T+1187 mimic) (Fig. 3). [score:3]
At 50 nM the miRA-1187 mimic with 12 h transfection resulted in the highest expression of miR-1187 in BNLCL2 cells (P<0.001, Fig. 2a and b). [score:3]
qPCR was used to confirm the expression of miR-1187 and caspase-8 mRNA. [score:3]
In order to study the regulatory role of miR-1187 in caspase-8, we applied a miR-1187 mimic for study. [score:2]
It was shown that miR-1187 was overexpressed in BNLCL2 cells transfected with miR-1187 mimic compared with the cells transfected with NSM. [score:2]
The miR-1187 expression signal was quantified and about a 97 or 96% decrease at 5 or 7 h post challenge respectively was noted compared with 0 h (saline control) (P<0.001, Fig. 1e). [score:2]
In the current study, we report a possible role of miR-1187 in hepatocyte apoptosis in ALF mice. [score:1]
In order to study the role of miR-1187 in hepatocytes apoptosis, D-GalN/TNF was applied to induce BNLCL2 to apoptosis. [score:1]
org/) revealed that 7 nucleotides in the seed region of miR-1187 were complementary to the position 192–198 of caspase-8 mRNA 3′UTR in mice (Fig. 1f). [score:1]
The synthesized miR-1187 mimic and non-specific mimic (NSM) were purchased from RiboBio (Guangzhou, China). [score:1]
LNA -based microarray analysis showed that hepatic miR-1187 was dramatically decreased with ongoing ALF (Fig. 1d). [score:1]
miR-1187 was dramatically decreased in the cells induced with D-GalN/TNF (P<0.01, Fig. 2c), correlating with our in vivo data above (Fig 1d, e and g). [score:1]
Transfection of the miR-1187 mimic and non-specific mimic. [score:1]
BNLCL2 cells (50–70% confluent) were transiently transfected with miR-1187 mimic (50 nM) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instruction; NSM (50 nM) was transfected as a negative control. [score:1]
It was detected that miR-1187 was reduced in the liver of ALF mice by qRT-PCR. [score:1]
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2
[+] score: 23
miR-466 and miR-1187, in particular, emerge as significant and regulators, sharing common target genes with Nr2e3, Rora, Ezh2 that are also differentially expressed in AMD. [score:6]
miR-1187 potential targets include Carboxypeptidase M (Cpm) a protein coding gene involved in monocyte and macrophage differentiation [97], Ectonucleoside triphosphate diphosphohydrolase 1 (Entpd1) which functions in the hydrolyzes of phosphates into ATP, ADP, and AMP [98], and Fraser extracellular matrix complex subunit 1(Fras1) that encodes for an extracellular matrix protein that regulates the epidermal-basement membrane adhesion as well as organogenesis in development [99]. [score:5]
Current studies show miR-1187 is down-regulated in a mouse mo del for acute liver failure and plays a role in hepatocyte apoptosis [96]. [score:4]
Additionally, prior studies from various mo dels of retinal degeneration identified over 300 differentially expressed miRNAs 63– 90, a total of 16 common miRNAs were identified (miR-1187, miR-125b-5p, miR-331-3p, miR466d-3p, miR-467f, miR-542-3p, miR-574-5p, miR654-3p, miR669h-3p, miR-882, miR-342-3p, miR-466a-5p, miR-466d-5p, miR-706, miR-345-3p, miR532-5p). [score:3]
This filtering strategy allowed us to identify 2 genes (Ell2 and Entpd1) as well as 3 miRNAs (mir466, miR1187 and miR710) that are regulated by epigenetic factors and nuclear hormone receptors and are associated with AMD pathogenesis. [score:2]
Yu DS The regulatory role of microRNA-1187 in TNF-?? [score:2]
miR-1187 has no known function in the retina. [score:1]
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3
[+] score: 13
Moreover, the down-regulated expression levels of miR-1187 may reflect diminished hepatocyte apoptosis through targeting casp-8 (Yu et al., 2012). [score:8]
Nd11 Function PMID miR-188-5p −65 Acts as a tumor suppressor in prostate caner 25714029 miR-1187 −54 Involved in hepatocyte apoptosis 22266786 miR-1196-5p −37 Unknown miR-211-3p −55 lncRNA-uc002kmd. [score:3]
The regulatory role of microRNA-1187 in TNF-α -mediated hepatocyte apoptosis in acute liver failure. [score:2]
[1 to 20 of 3 sentences]
4
[+] score: 7
In Group 1, the most significantly up-regulated miRNAs were miR-1187, miR-125a-3p, miR-466c-5p, miR-5105 and miR-3472, whereas the most significantly down-regulated was miR-125b-5p. [score:7]
[1 to 20 of 1 sentences]
5
[+] score: 7
The ten most up-regulated miRNAs included mmu-miR-205-5p, mmu-miR-222-3p, mmu-miR-205-3p, mmu-miR-146b-5p, mmu-miR-21-5p, mmu-miR-21-3p, mmu-miR-221-3p, mmu-miR-140-3p, mmu-miR-142-5p, and mmu-miR-140-5p and the ten most down-regulated miRNAs comprised mmu-miR-211-5p, mmu-miR-3096-5p, mmu-miR-711, mmu-miR-466h-5p, mmu-miR-130b-3p, mmu-miR-3082-5p, mmu-miR-1199-5p, mmu-miR-669b-5p, mmu-miR-1187, and mmu-miR-1224-5p (Table 1). [score:7]
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6
[+] score: 6
The five highest-ranking miRNA candidates that are binding targets of each circRNA were identified as: 1) For mmu_circRNA_40001:mmu-miR-466f, mmu-miR-466i-5p, mmu-miR-669n, mmu-miR-1187, and mmu-miR-466c-5p; 2) For mmu_circRNA_013120: mmu-miR-6541, mmu-miR-669c-3p, mmu-miR-466f-5p, mmu-miR-669m-5p, and mmu-miR-466j; and 3) For mmu_circRNA_40806: mmu-miR-7038-3p, mmu-miR-20a-3p, mmu-miR-145a-3p, mmu-miR-346-3p, and mmu-miR-149-5p. [score:3]
The results indicate that the potential miRNA targets include mmu-miR-466j, mmu-miR-669m-5p, mmu-miR-466f-5p, mmu-miR-1187, mmu-miR-466f, mmu-miR-466i-5p, mmu-miR-669n, and mmu-miR-466c-5p, exhibiting a larger interaction network. [score:3]
[1 to 20 of 2 sentences]
7
[+] score: 3
miRNA Fold change at 3 dpi Fold change at 5 dpi mmu-miR-466h-3p NS (Not significant) 14.311053 mmu-miR-346-5p NS 3.4766614 mmu-miR-877-3p NS 3.416667 mmu-miR-7a-5p NS 2.1413074 mmu-miR-5107-5p NS −2.047792 mmu-miR-3473a −2.2872427 −2.1317267 mmu-miR-150-5p NS −2.1770155 mmu-miR-3473b −3.2475147 −2.282881 mmu-miR-721 NS −2.6864858 mmu-miR-669b-5p NS −2.9408455 mmu-miR-709 NS −3.0065749 mmu-miR-669n NS −3.0094464 mmu-miR-468-3p NS −3.40051 mmu-miR-466m-5p NS −4.33538 mmu-miR-32-3p NS −4.5324426 mmu-miR-466h-5p NS −4.9673104 mmu-miR-3082-5p NS −6.01648 mmu-miR-466i-5p NS −7.6776285 mmu-miR-1187 NS −8.772696 mmu-miR-574-5p NS −9.259378 To confirm the validity of the differentially expressed miRNAs that had been identified by microarray analysis, we performed real-time PCR on all 20 of these miRNAs using the polyA tailing technique. [score:3]
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8
[+] score: 1
Nine microRNAs (miR-1, miR-1187, miR-133a, miR-133b, miR-155, miR-2137, miR-223, miR-30d and miR-574-3p) were selected for validation. [score:1]
[1 to 20 of 1 sentences]
9
[+] score: 1
Tg2576 mice, only miR-1187 was significantly decreased. [score:1]
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