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1 publications mentioning dme-mir-988Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-988. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: dme-mir-3, dme-mir-4, dme-mir-5, dme-mir-6-1, dme-mir-6-2, dme-mir-6-3, dme-mir-281-1, dme-mir-34, dme-mir-92b, dme-mir-286, dme-mir-309, dme-mir-310, dme-mir-193, dme-mir-1000
Females that overexpress mir-34 and mir-92b lay 34% and 37% fewer eggs after mating, whereas females that overexpress mir-286 and mir-988 lay 16% and 33% more eggs than controls (Figure 7).
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Mating causes a reduction in expression of mir-34, mir-92b and mir-988 and an increase in expression of mir-286 (Figure 3; Additional file 4).
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To test the connectivity of the computational networks (Figures 4, 5 and 6) and evaluate causality, we overexpressed six miRNAs for which overexpression lines were available (mir-281-1, mir-286, mir-34, mir-92b, mir-310 and mir-988) using the binary GAL4-UAS expression system [38] under a universal ubiquitin promoter.
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Number of eggs laid by five young mated females over 18 hours between control and mir-286, mir-34, mir-92b and mir-988 overexpression lines.
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To assess causality between post-mating modulation of miRNA expression and oviposition, we measured egg laying after mating by control females and females overexpressing mir-286, mir-34, mir-92b, or mir-988.
[score:3]
We obtained UAS-mir-281-1, UAS-mir-286, UAS-mir-34, UAS-mir-92b, UAS-mir-988, mir-310 and the co-isogenic control lines from the Bloomington Stock Center.
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