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8 publications mentioning mmu-mir-465c-1

Open access articles that are associated with the species Mus musculus and mention the gene name mir-465c-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 26
B) Pri-miRNA FISH images of pachytene cells expressing miR-465 in Spo11 null and Brca1 [Δ11/Δ11] mutants (defective in XY silencing). [score:3]
S5 FigCombined DNA / pri-miRNA FISH for miR-465 shows expression of miR-465 from the autosomal, transgenic locus (arrows) but not from the X locus (arrowhead). [score:3]
We targeted the X-miRNA miR-465, present in six copies in cluster 4 (Fig 1D). [score:3]
MiR-465 expression pattern recapitulates that of cluster 4 miRNAs (Fig 1D). [score:3]
This proved to be the case: in the few Spo11 null and Brca1 pachytene cells in which the miR-465 locus, identified using DNA FISH, lay within a γH2AFX domain (Spo11 null: n = 2 out of 54 cells; Brca1 [Δ11/Δ11]: n = 2 out of 15 cells), no pri-miR-465 expression could be observed (Fig 2C). [score:3]
S4 FigPri-miRNA FISH shows that miR-465 is silent in wild type pachytene cells. [score:1]
Detection of pri-miR-465 by pri-miRNA FISH in miRBAC line 1 transgenic pachytene spermatocytes. [score:1]
Top: Four variants of the miR-465 gene are repeated in the genome at the XA7 locus (see Fig 1D). [score:1]
Pri-miRNA FISH shows that miR-465 is silent in wild type pachytene cells. [score:1]
For miR-465-specific pri-miRNA FISH, a mix of nine amino-allyl -modified oligonucleotides labeled with fluorolink Cy3 were used as probes (S1 Table). [score:1]
Pri-miR-465-specific oligonucleotide probes and BAC probes were used for RNA and DNA FISH respectively. [score:1]
We observed the same results using our miR-465-specific pri-miRNA FISH protocol on X-miRBAC line 1 (S5 Fig) (100%, n = 9). [score:1]
We subsequently repeated the analysis of cluster 4 X-miRNAs using our oligonucleotide RNA FISH approach that specifically detects pri-miRNAs for the six copies of miR-465. [score:1]
Global DNA alignment of the precursors of the miR-465 variants is shown. [score:1]
C) In some rare pachytene cell, the miR-465 locus is found underneath the silencing body that forms in Spo11 null and Brca1 [Δ11/Δ11] spermatocytes. [score:1]
In addition, we performed miR-465-specific pri-miRNA FISH in a second MSCI mutant, the Brca1 [Δ11/Δ11] mo del [17, 18]. [score:1]
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2
[+] score: 16
The miRNA expression profiling of sterile (PWD×B6)F1 and fertile (B6×PWD)F1 14.5dpp testes showed 1.5- to 2-fold upregulation of Mir883b-3p, Mir465a/b/c-3p and Mir465a/b-5p in sterile males, while Mir743a, Mir743-5p, Mir880 and Mir465c-5p showed 1.2- to 4-fold down-regulation (Figure 3C, D). [score:9]
The critical region also contains the Mir465 cluster of miRNA genes, which show a significant difference in expression between reciprocal F1 hybrids. [score:3]
Significant overexpression of Mir465 cluster in PWD germ cells is shown in red. [score:2]
Only the Mir465 [PWD] cluster displayed a significant increase of expression in the first meiotic prophase of PWD and B6 sorted testicular cells (Figure 3A, B). [score:2]
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3
[+] score: 15
Similarly, 342 genes potentially downregulated (targets of mmu-miR-128 and mmu-miR-342-5p) and 200 potentially upregulated genes (targets of mmu-miR-465c-5p, mmu-miR-466d-3p, mmu-miR-466d-5p, mmu-miR-665, mmu-miR-683) in the colon were identified (Table S4). [score:11]
qRT-PCR was performed using SYBR Green qPCR Master Mix (Fermentas) on a Mastercycler Realplex [4] (Eppendorf) using the following primers:For mature miRNA expression: the universal primer provided in the NCode [TM] miRNA first-strand cDNA synthesis kit was used together with one of the following forward primer: mmu-miR-665: 5′-ACCAGG AGG CTG AGG TCC CT-3′mmu-miR-128: 5′-TCACAGTGAACCGGTCTCTTT-3′ mmu-mir-200c*: 5′-CGTCTTACCCAGCAGTGTTTGG-3′ mmu-miR-342-5p: 5′-AGGGGTGCTATCTGTGATTGAG-3′ mmu-miR-466d-3p: 5′-TATACATACACGCACACATAG-3′ mmu-miR-466d-5p: 5′-TGTGTGTGCGTACATGTACATG-3′ mmu-miR-465c-5p: 5′-TATTTAGAATGGCGCTGATCTG-3′ mmu-miR-683: 5′-CCTGCTGTAAGCTGTGTCCTC-3′ mmu-miR-665: 5′-ACCAGGAGGCTGAGGTCCCT-3′ mmu-miR-298: 5′-GGCAGAGGAGGGCTGTTCTTCCC-3′ For Abcc3 expression:Forward 5′-CTT CTT TTC CCG CTT GTC TTT-3′;Reverse 5′- CCT CCT CAG ACA GAG ACC AGA-3′. [score:2]
qRT-PCR was performed using SYBR Green qPCR Master Mix (Fermentas) on a Mastercycler Realplex [4] (Eppendorf) using the following primers: For mature miRNA expression: the universal primer provided in the NCode [TM] miRNA first-strand cDNA synthesis kit was used together with one of the following forward primer: mmu-miR-665: 5′-ACCAGG AGG CTG AGG TCC CT-3′ mmu-miR-128: 5′-TCACAGTGAACCGGTCTCTTT-3′ mmu-mir-200c*: 5′-CGTCTTACCCAGCAGTGTTTGG-3′ mmu-miR-342-5p: 5′-AGGGGTGCTATCTGTGATTGAG-3′ mmu-miR-466d-3p: 5′-TATACATACACGCACACATAG-3′ mmu-miR-466d-5p: 5′-TGTGTGTGCGTACATGTACATG-3′ mmu-miR-465c-5p: 5′-TATTTAGAATGGCGCTGATCTG-3′ mmu-miR-683: 5′-CCTGCTGTAAGCTGTGTCCTC-3′ mmu-miR-665: 5′-ACCAGGAGGCTGAGGTCCCT-3′ mmu-miR-298: 5′-GGCAGAGGAGGGCTGTTCTTCCC-3′ For Abcc3 expression: Forward 5′-CTT CTT TTC CCG CTT GTC TTT-3′; Reverse 5′- CCT CCT CAG ACA GAG ACC AGA-3′. [score:2]
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4
[+] score: 14
Several miRNAs, such as members of the miR-183-96-182 cluster, miR-741, miR-881, miR-878, miR-743a, miR-743b, miR-465, miR-871, miR-880, are known to be expressed in mouse ESCs and were also expressed in rat PSCs; however, their function in pluripotency induction and regulation has not been directly shown 48– 50. [score:7]
In addition, we found that the rno-miR-novel-8 miRNA was located less than 10 kb from miR-871, mir-3580 and miR-465, which were upregulated in rat PSCs. [score:4]
A cluster of miRNAs, comprised of miR-463, miR-465, miR-471, miR-741, miR-743a, miR-743b, miR-871, miR-878, miR-880, miR-881, and miR-883, was located on the X chromosome and was expressed at a high level in rat PSCs according to the sequencing data. [score:3]
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5
[+] score: 12
Several members of the miR-465 and miR-743 families were up-regulated in both young and aged df/df mice, although they were expressed at lower levels than the previously discussed miRNAs. [score:6]
miR-465 was previously detected as one of the most highly expressed miRNAs in the newborn mouse ovary, despite being undetectable in the adult ovary [6]. [score:3]
The mir-465 cluster is located in the X chromosome, and is strongly expressed in the testis, although not detected in other tissues [6]. [score:3]
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6
[+] score: 7
For instance, mmu-miR-290 was up-regulated in all organs except forestomach; mmu-miR-465 was down-regulated in lung, spleen and glandular stomach. [score:7]
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7
[+] score: 2
Eleven miRNAs were randomly selected for qPCR analysis for each age: GD 17.0 miR-1843-5p, miR-485-3p, miR-711, miR-3962, miR-3067-3p, miR-212-3p, miR-669i, miR-877, miR-26b-3p, miR-465c-3p, let-7b-3p; GD 18.0 miR-1843-5p, miR-485-3p, miR-3473d, miR-132-5p, miR-3074-1-3p, miR-128-2-5p, miR-130b-5p, miR-490-5p, miR-669h-3p, miR-3058-5p, miR-146b. [score:1]
A statistically significant effect of flutamide was observed for seven of these miRNAs (miR-26b-3p, let-7b-3p, miR-465c-3p, miR-669h-3p, miR-3058-5p, miR-146b, miR-1843-5p), and a trend toward a statistically significant effect was observed for another miRNA (miR-130b-5p) (Fig.   2b, c). [score:1]
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8
[+] score: 1
Other miRNAs from this paper: mmu-mir-30a, mmu-mir-30b, mmu-mir-141, mmu-mir-151, mmu-mir-10b, mmu-mir-191, mmu-mir-143, mmu-mir-30e, mmu-mir-34c, mmu-mir-34b, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-mir-21a, mmu-mir-10a, mmu-mir-139, mmu-mir-375, mmu-mir-196b, mmu-mir-465a, mmu-mir-466a, mmu-mir-467a-1, mmu-mir-669a-1, mmu-mir-669b, mmu-mir-669a-2, mmu-mir-669a-3, mmu-mir-467b, mmu-mir-669c, mmu-mir-465b-1, mmu-mir-465b-2, mmu-mir-465c-2, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-467c, mmu-mir-467d, mmu-mir-466d, mmu-mir-208b, mmu-mir-467e, mmu-mir-466l, mmu-mir-669k, mmu-mir-669g, mmu-mir-669d, mmu-mir-466i, mmu-mir-669j, mmu-mir-669f, mmu-mir-669i, mmu-mir-669h, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-467f, mmu-mir-466j, mmu-mir-669e, mmu-mir-467g, mmu-mir-467h, mmu-mir-669l, mmu-mir-669m-1, mmu-mir-669m-2, mmu-mir-669o, mmu-mir-669n, mmu-mir-466m, mmu-mir-669d-2, mmu-mir-466o, mmu-mir-467a-2, mmu-mir-669a-4, mmu-mir-669a-5, mmu-mir-467a-3, mmu-mir-466c-2, mmu-mir-669a-6, mmu-mir-467a-4, mmu-mir-466b-4, mmu-mir-669a-7, mmu-mir-467a-5, mmu-mir-466b-5, mmu-mir-669p-1, mmu-mir-467a-6, mmu-mir-669a-8, mmu-mir-466b-6, mmu-mir-669a-9, mmu-mir-467a-7, mmu-mir-466b-7, mmu-mir-669p-2, mmu-mir-467a-8, mmu-mir-669a-10, mmu-mir-467a-9, mmu-mir-669a-11, mmu-mir-467a-10, mmu-mir-669a-12, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, mmu-mir-466q, mmu-mir-6240, mmu-mir-30f, mmu-mir-465d, mmu-mir-466c-3
Prominent among these were members of the let7, miR-30, miR-465, miR-466, miR-467, and miR-669 clusters. [score:1]
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