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11 publications mentioning osa-MIR820b

Open access articles that are associated with the species Oryza sativa and mention the gene name MIR820b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 245
Other miRNAs from this paper: osa-MIR820a, osa-MIR820c
The expression level of pre-miR820 was normalized to OsGAPDH and the expression level of the empty-vector lines was set as 1. (B) Northern blot analysis showing increased miR820 expression in two independent transgenic lines transformed with the pre-miR820 overexpression construct compared to empty-vector controls. [score:8]
We found that the expression levels of several TEs were increased in DRM2 RNAi transgenic lines; furthermore, the expression levels of TEs such as RIRE7 and CACTA carrying pre-miR820 were inversely related to the degree of DRM2 suppression (Figure 2C; Figure S6). [score:7]
Negative regulation of OsDRM2 by miR820 activates TE expressionTo test whether the expression level of OsDRM2 depends on recognition by miR820, we made transgenic rice plants that express a fusion of a green fluorescent protein (GFP) gene and OsDRM2 with or without synonymous mutations within the miR820 recognition site; we then observed the GFP fluorescence and measured GFP mRNA levels (Figure 2A, 2B). [score:7]
Furthermore, we also observed elevated expression of RIRE7 in the same pre-miR820 overexpression experiment in which OsDRM2 expression was found to be mildly reduced (Figure S5E). [score:7]
Transposon-derived miR820 targets de novo DNA methyltransferase gene OsDRM2 miRNAs are produced from stem structures formed within noncoding transcripts [10] and negatively regulate the expression of a range of plant genes, mainly by mRNA cleavage [11]. [score:6]
Figure S5Overexpression of pre-miR820 decreases OsDRM2 expression. [score:5]
Indeed, we found that overexpression of pre-miR820 under the control of a strong constitutive promoter mildly reduced but did not eliminate the expression of OsDRM2 (Figure S5A– S5D). [score:5]
When OsDRM2 expression decreases, possibly because of nucleotide substitutions within miR820 that enable it to form more stable hybrids with OsDRM2 or for other reasons, more miR820 can be produced, possibly because host -mediated silencing is suppressed efficiently. [score:5]
In wild-type plants, both miR820 and OsDRM2 were expressed in all the tissues tested, although their expression levels differed between tissues (Figure S4D, S4G). [score:5]
Indeed, RNAi -mediated suppression of OsDRM2 increased pre-miR820 expression (Figure S6C). [score:5]
This might be because the expression levels of miR820 and OsDRM2 differed between tissues, and because miR820 might reduce the amount of OsDRM2 expression but not abolish it completely. [score:5]
This suggests that the expression patterns of miR820 and OsDRM2 overlap at the cellular level, supporting the idea that miR820 regulates OsDRM2. [score:4]
However, considering that OsDRM2 gave the highest hit score when miR820 was used in BLAST searches against the entire rice genome (IRGSP Pseudomolecules 1.0) other than miR820 itself, it is very likely that miR820 negatively regulates the expression of OsDRM2 at least in part. [score:4]
As expected, the expression level of the OsDRM2:GFP fusion gene with an intact miR820 recognition site was much lower than for those with synonymous mutations. [score:4]
Negative regulation of OsDRM2 by miR820 activates TE expression. [score:4]
This number of substitutions could greatly affect the capability of miR820 to regulate DRM2 in species with those genomes; however, the degree of base-pairing between miR820 and its target site in DRM2 in the BB and BBCC genomes is conserved (Figure 3B; Table S1). [score:4]
To test whether the expression level of OsDRM2 depends on recognition by miR820, we made transgenic rice plants that express a fusion of a green fluorescent protein (GFP) gene and OsDRM2 with or without synonymous mutations within the miR820 recognition site; we then observed the GFP fluorescence and measured GFP mRNA levels (Figure 2A, 2B). [score:4]
Indeed, we also confirmed the function of the 24-nt miR820 species by detecting a high level of cytosine methylation specific to its presumed target site (Figure S4B). [score:3]
This is expected to drive the selection of nucleotide substitutions at miR820 or at its target site because drastic reduction of OsDRM2 levels could be lethal. [score:3]
Next, the part of each OsDRM2 cDNA that included the miR820 target site was amplified and cloned into the pENTR/D-TOPO vector (Invitrogen). [score:3]
In turn, TE would favor changes in the miR820 sequence that correspond to the changes in the target site. [score:3]
miR820 family members are located within CACTA transposons and target the DNA methyltransferase gene OsDRM2. [score:3]
Table S1Sequencing analysis of miR820 and its target site in DRM2 among various Oryza species. [score:3]
Our mo del for the regulation of DRM2 by miR820 predicts that this regulation might affect not only CACTA carrying pre-miR820 but also other TEs. [score:3]
Total RNA was isolated from shoots of waf1 and various tissues of Nipponbare wild-type non-transgenic plants; shoots of p35S:OsDRM2 intact:GFP, p35S:OsDRM2 mutation1:GFP, and p35S:OsDRM2 mutation2:GFP T [2] plants; and calli of pAct:pre-miR820 and pAct:OsDRM2 RNAi by using TRIzol reagent (Invitrogen). [score:3]
However, considering that BB-containing species have relatively small genomes compared with other Oryza species [26], the downregulation of DRM2 by miR820 would not be expected to affect a large number of TEs in BB-containing species. [score:3]
This indicates that, in BB/BBCC Oryza species, the sequences of miR820 and its target site in DRM2 have co-evolved to maintain the ability to form a stable RNA–RNA duplex. [score:3]
This action can be blocked by miR820 (thin red line), which suppresses the host's silencing machinery and can drive host genome evolution (thick red arrow). [score:3]
The sequences of miR820 and its target site in OsDRM2 have co-evolved in BB/BBCC Oryza speciesWe did not find miR820 or its precursor sequence in the Arabidopsis or maize genome, suggesting that miR820 is not wi dely conserved in plants. [score:3]
This might account for the fact that, in BB species, the most predominant CACTAs with pre-miR820 were those that could form the most stable hybrid with the target sequence. [score:3]
Boxes indicate the types of miR820 sequences and DRM2 target site sequences identified in each genome (right). [score:3]
Figure S4The target of miR820 is OsDRM2. [score:3]
1002953.g001 Figure 1 miR820 family members are located within CACTA transposons and target the DNA methyltransferase gene OsDRM2. [score:3]
Transposon-derived miR820 targets de novo DNA methyltransferase gene OsDRM2. [score:3]
Because of this homology, miR820 is predicted to target Os03g0110800 (OsDRM2), which encodes a de novo DNA methyltransferase orthologous to Arabidopsis DRM1/2 [15]– [19] (Figure S4A). [score:3]
The sequences of miR820 and its target site in OsDRM2 have co-evolved in BB/BBCC Oryza species. [score:3]
It has been reported that the 24-nt species of miR820 acts as a guide for DNA methylation at its target site, possibly through RdDM [13]. [score:3]
The target site of miR820 is indicated by the red bar. [score:3]
On the other hand, we did not observe a clear inverse relationship between the levels of miR820 and OsDRM2 expression. [score:3]
Next, we tested whether the expression patterns of OsDRM2 and miR820 overlapped. [score:3]
Figure S7Sequence alignments of miR820 and its target site in DRM2 among Oryza species. [score:3]
In waf1, the expression levels of both the 22-nt and 24-nt species of miR820 decreased compared to the wild-type (Figure 1B). [score:2]
1002953.g002 Figure 2 OsDRM2 is negatively regulated by miR820. [score:2]
OsDRM2 is negatively regulated by miR820. [score:2]
In fact, the majority (11 out of 18 copies) of pre-miR820 found in the BB genome carries the same miR820 sequence as the one recovered by direct sequencing, which is also the sequence that would form the most stable hybrid with the DRM2 sequence found in the BB genome. [score:2]
Thus, in the BB and BBCC genomes, there are eight nucleotide substitutions in miR820 and its recognition site in DRM2, compared with the corresponding miR820 and target sequences in Nipponbare. [score:2]
A mo del for the regulation of DRM2 by miR820 sequences is shown in Figure 4D. [score:2]
Because pre-miR820 loci simultaneously produce both 22-nt and 24-nt miRNA species (Figure 1B), we investigated whether the 22-nt miR820 species regulates OsDRM2 expression through mRNA degradation by mapping the 22-nt miR820 cleavage site of OsDRM2. [score:2]
1002953.g003 Figure 3Regulation of DRM2 by miR820 is conserved among Oryza species. [score:2]
Thus, our analysis of the regulation of DRM2 by miR820 sheds light on the action of two types of transposon-derived small RNAs, siRNA and miRNA, in the battles and possibly even the cooperation between plant genomes and their parasites. [score:2]
Because the miR820 sequence in the BB species shown in Figure 3B was obtained by direct sequencing of PCR products, it should be representative of the miR820 sequence in BB species. [score:2]
We successfully amplified and sequenced both miR820 and its recognition site in DRM2 from the genomic DNAs of various accessions of Oryza [24] (Figure S7A, S7B; Table S1), strongly suggesting the conservation of this regulation mechanism among Oryza species. [score:2]
The co-evolution of these sequences strongly suggests that the regulation of DRM2 by miR820 is functional and that those nucleotide changes have accumulated as a result of the interplay between the host genome and the parasitic elements in these species. [score:2]
Regulation of DRM2 by miR820 is conserved among Oryza species. [score:2]
We amplified both miR820 and its target site in DRM2 by PCR using the primers listed in Table S2. [score:2]
It is possible that, in order to adapt against genomic stresses such as climate or environmental changes, the host maintained or created genome flexibility by keeping or allowing DRM2 under the regulation of miR820 in BB species in the past. [score:2]
We then tested whether regulation by miR820 is conserved among various Oryza species. [score:2]
To see whether co-evolution of the nucleotide sequences of DRM2 and miR820 affected the behavior of TEs carrying pre-miR820 in the BB genome, we performed Southern blot analysis to detect the copy number of CACTA carrying pre-miR820 (Figure 4A). [score:1]
Red triangles indicate the bands corresponding to the five copies of pre-miR820 in Nipponbare. [score:1]
This analysis revealed a sudden increase in copy number of CACTA carrying pre-miR820, in which identical sequences around the pre-miR820 region were recovered from multiple loci (Figure 4C). [score:1]
Figure S1Structures and sequences of the five copies of miR820 in Nipponbare rice. [score:1]
Figure S2Multiple sequence alignment of the five copies of pre-miR820 in the Nipponbare genome. [score:1]
The miRNA target score was calculated for each miR820: DRM2 duplex based on the method described in [30]. [score:1]
It is possible that this increase was not due solely to the loss of miR820 because in waf1, the levels of most other small RNAs are also reduced [20]. [score:1]
We also sequenced the CACTA with pre-miR820 in BB-genome species and conducted phylogenetic analysis using pre-miR820 sequences from Nipponbare and BB-genome species. [score:1]
The blue lines, red box, and black box indicate the regions corresponding to the stem-loop structure, miR820, and miR820*, respectively. [score:1]
By screening these libraries using a labeled pre-miR820 DNA fragment, we identified 48 BAC clones carrying miR820-CACTA. [score:1]
We confirmed that these clones carried miR820-CACTA by PCR amplification and sequencing of the region around pre- miR820 in CACTA. [score:1]
Briefly, by screening the BAC library of a BB-genome species, we identified BAC clones carrying miR820-CACTA from BB. [score:1]
We further confirmed that this cleavage depends on the presence of miR820 by using the waf1 mutant in rice [20] (Figure 1B–1D). [score:1]
Black and red numbers in parentheses indicate the chromosome locations of pre-miR820 sequences in the AA and BB genomes, respectively. [score:1]
Increased copy number of CACTA carrying pre-miR820 in the BB/BBCC genome. [score:1]
There are five copies of the CACTA transposon containing the miR820 precursor (pre-miR820) in the rice (Oryza sativa L. ) Nipponbare genome [14] (Figure S1A, S1B). [score:1]
Considering that miR820 is encoded by parasitic DNA and its primary function seems to be as an anti-host agent, it is possible that miR820 might be lost in the future, as is often the case for non-conserved miRNA genes. [score:1]
Considering the phylogenetic relationships among Oryza species [24], the miR820 sequence recovered from BB/BBCC Oryza species has diverged from miR820a/b/c (Figure 3A). [score:1]
TEs carrying pre-miR820 have proliferated in BB-genome species. [score:1]
So far, miR820 has been found only in rice, suggesting a recent origin. [score:1]
Thus, pre-miR820 possibly originated from Os03g0110800, and the number of pre-miR820 copies increases as the CACTA TEs propagate. [score:1]
To confirm that OsDRM2 mRNA cleavage depends on the presence of miR820, we checked for the cleavage product in waf1 mutants and in the wild-type. [score:1]
These results suggest that the CACTA transposon with this miR820 sequence was predominantly proliferated or maintained, and became the predominant miR820 in BB species. [score:1]
The second and third rows indicate the similarity between the sequences of the miR820 precursor (pre-miR820) and Os03g0110800 (OsDRM2). [score:1]
1002953.g004 Figure 4Increased copy number of CACTA carrying pre-miR820 in the BB/BBCC genome. [score:1]
To detect the copy number of CACTA TEs carrying miR820 by Southern blot analysis, genomic DNA samples were extracted from leaves of Nipponbare (AA), W1514 (BB), W1331 (BBCC), and W1805 (CC), treated with RNase A, and digested with restriction enzymes. [score:1]
Figure S3Multiple sequence alignment of the five copies of pre-miR820 and a part of the OsDRM2 sequence in the Nipponbare genome. [score:1]
Genomic DNA from AA, BB, BBCC, and CC Oryza species were digested with the enzymes indicated and probed with pre-miR820. [score:1]
The blue line, black lines, and red box indicate the regions corresponding to the OsDRM2 second exon, third intron, and miR820*, respectively, in the pre-miR820 sequences and the miR820 recognition site in OsDRM2. [score:1]
We also successfully determined the genomic locations of CACTA with pre-miR820 in the BB genome (see for details) and found that at least 18 copies of CACTA with pre-miR820 are dispersed throughout this genome (Figure 4B). [score:1]
Mapping of CACTA carrying pre-miR820. [score:1]
In the waf1 mutants, there was no detectable cleavage of OsDRM2 mRNA by miR820 (Figure 1C). [score:1]
Our strategy to map miR820-CACTA from BB-genome species was based on the synteny between AA and BB Oryza species [26]. [score:1]
For pAct:pre-miR820:Nos construction, a 0.5-kb pre-miR820 fragment was amplified and inserted into the pCRII vector (Invitrogen). [score:1]
For analysis of waf1 and wild-type plants and of pAct:pre-miR820:Nos and pAct:OsDRM2 RNAi:Nos callus, 10 µg of each RNA sample was loaded onto an agarose or acrylamide gel (for analysis of OsDRM2 and miR820a/b/c, respectively), separated by electrophoresis, and blotted onto nylon membranes. [score:1]
There are also several nucleotide substitutions in the miR820 recognition site in DRM2 in some Oryza genomes (Figure S7B). [score:1]
miR820 is a small -RNA species with sizes of 22 and 24 nt [12], [13]. [score:1]
The genomic locations of CACTA carrying pre-miR820 in AA and BB Oryza species are shown by gray arrows and black arrows, respectively. [score:1]
However, it is intriguing to speculate that miR820 might function not only as an anti-host mechanism for parasites but also in a way that is beneficial for the host. [score:1]
The primary and secondary structures of pre-miR820 also support this idea, because pre-miR820 still shows high homology within its stem parts to the intron sequence of OsDRM2. [score:1]
Mapping of CACTA carrying pre-miR820 Our strategy to map miR820-CACTA from BB-genome species was based on the synteny between AA and BB Oryza species [26]. [score:1]
We found that the copy number of CACTA with pre-miR820 was much higher in the BB/BBCC Oryza species than in the AA species Nipponbare. [score:1]
We hypothesize the following scenario as a mechanism connecting the co-evolution of miR820 and DRM2 and the rapid increase in the copy number of CACTA carrying pre-miR820. [score:1]
The co-evolution of miR820 and its recognition site in BB/BBCC species supports this idea. [score:1]
miR820 is produced from transcripts originating from a region inside a class of CACTA DNA transposons in rice (Figure 1A). [score:1]
We did not find miR820 or its precursor sequence in the Arabidopsis or maize genome, suggesting that miR820 is not wi dely conserved in plants. [score:1]
The miR820 sequences are designated by red rectangles. [score:1]
The nucleotide sequences of the fold-back region of all five pre-miR820 sequences show high sequence similarity to parts of Os03g0110800 and the homologous region extends into the second exon and third intron of Os03g0110800 (Figure 1A; Figures S2, S3). [score:1]
TEs carrying pre-miR820 have proliferated in BB-genome speciesTo see whether co-evolution of the nucleotide sequences of DRM2 and miR820 affected the behavior of TEs carrying pre-miR820 in the BB genome, we performed Southern blot analysis to detect the copy number of CACTA carrying pre-miR820 (Figure 4A). [score:1]
These samples were loaded onto an agarose gel, separated by electrophoresis, blotted onto a nylon membrane, and probed with the pre-miR820 DNA fragment. [score:1]
A pre-miR820 fragment was then excised with XbaI and SmaI, and cloned into the binary vector carrying the rice Actin gene promoter and Nos terminator. [score:1]
The red triangle indicates the location of miR820 within the stem-loop region. [score:1]
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[+] score: 213
In PB1, Osa-miR820 gets down-regulated leading to increase in OsDRM2 expression levels, while in PK Osa-miR820 gets up-regulated leading to decrease in OsDRM2 expression levels (Figure 5D). [score:11]
On examining the relative expression patterns of Osa-miR820 length variants in response to salt stress it became apparent that the 21-nt miR was down-regulated in PB1 causing a relative up-regulation of the 24-nt length variant (Figure 2B). [score:9]
Expression of miR820 and Its Targets in Diverse Rice Cultivars. [score:5]
Expression of miR820 and Its Targets Under Other Abiotic Stresses. [score:5]
Considering the observations that Osa-miR820 is expressed during early reproductive developmental stages and is deregulated under salt stress, we investigated its expression profiles in various floral tissues under salt stress conditions, using both salt-susceptible (PB1) and salt-tolerant (PK) cultivars (Figure 5). [score:5]
Expression of miR820 and Its Targets in Different Tissues. [score:5]
FIGURE 4 Expression of Osa-miR820 and its target, OsDRM2 in various tissues of Pusa Basmati 1. (A) The vegetative tissues from 15 days old seedlings and mature plant. [score:5]
Thus, both 21- and 24-nt Osa-miR820 length variants seems to regulate the same target though at different points indicating that the dynamics of the plant genetic machinery may require dual mode of regulation. [score:5]
In pre-pollinated floral tissues, salt stress induces the expression of Osa-miR820 in PB1, with corresponding decrease in OsDRM2 expression levels (Figure 5C). [score:5]
FIGURE 5 Expression profiling of Osa-miR820 and its target, OsDRM2 in different floral tissues of Pusa Basmati 1 (PB) and Pokkali (PK) under control (N) and salt stressed (S) conditions. [score:5]
In the presence of physiological drought conditions, the Osa-miR820 levels were up-regulated in the leaves till 3 h beyond which these reduced drastically. [score:4]
The profiling of Osa-miR820 and its target transcript in the different tissues enabled the identification of its regulatory zones. [score:4]
It will also be interesting to decipher the expression zones of individual Osa-miR820 loci and understand their deregulation in stress. [score:4]
This indicates that the Osa-miR820 family might be involved in a dual mode of target regulation. [score:4]
The results indicate that Osa-miR820 expression is precisely regulated in the different tissues of rice. [score:4]
The general pattern that emerged from the time kinetics studies suggests a stress induced increase in the Osa-miR820 levels in PB1 and PK leaves accompanied by a down-regulation of OsDRM2. [score:4]
The analysis was extended to understand the deregulation of Osa-miR820 and its target under increasing duration of salt stress, in 14-day-old seedlings of PB and PK. [score:4]
This suggests that though the expression of Osa-miR820 is deregulated by salt stress and the ratios between the 21-nt and 24-nt length variants may be playing an important role in governing the plants response to salt stress. [score:4]
In this study we describe the detailed expression profile of a salt stress deregulated Osa-miR820. [score:4]
The analysis was extended to understand the deregulation of Osa-miR820 and its target under high temperature and drought stress. [score:4]
A similar observation for Osa-miR820 was made in the salt-tolerant glyoxalase over -expressing transgenics (Singla-Pareek et al., 2008). [score:3]
The EST analysis also identified a putative NAC domain containing protein 77 (N77) and a putative aquaporin, PIP1-5, as targets of Osa-miR820. [score:3]
The time kinetics of salt stress revealed an increase in levels of Osa-miR820 in PK leaves similar to the pattern observed in PB1 though there was difference in the level of miR expression (Figure 6C). [score:3]
The expression levels of Osa-miR820 were also checked within the developing floral tissues (Figure 4B). [score:3]
Interestingly, the 24-nt Osa-miR820 family members were also shown to target and repress the OsDRM2 at the transcriptional level (Nosaka et al., 2013b). [score:3]
The copy number of rice CACTA DNA transposons carrying MIR820 does not correlate with MIR820 expression. [score:3]
This differential expression of Osa-miR820 in different zones of panicle indicates an important role of Osa-miR820 in controlling rice panicle formation. [score:3]
This indicates a delicate but controlled regulation of Osa-miR820 under changing developmental stages and time. [score:3]
Expression of the rice microRNA miR820 is associated with epigenetic modifications at its own locus. [score:3]
Digital Expression Status of Osa-miR820. [score:3]
This ambiguity may be due to overlapping expression domains of individual miR loci as the amplifications provided an overall status of the miR family due to high sequence similarity of the various Osa-miR820 isoforms. [score:3]
Here, we have investigated its role in salt, high temperature and drought stress responses by comparing the expression profiles of Osa-miR820 and its target gene across various tissues of two indica rice cultivars exhibiting a contrasting response to salt stress. [score:3]
Knowledge on the delicate balance in the ratios of the 21- and 24-nt miRs and their influence on the transcript expression and accumulation is required to reveal the intricate genetic reprogramming mediated by the Osa-miR820. [score:3]
In the root tissues of PB1 the expression of Osa-miR820 decreased though the concomitant increase in the levels of OsDRM2 was not observed (Figure 7B). [score:3]
The digital expression status of Osa-miR820 family, in the leaf and panicle tissues of different indica rice cultivars, was obtained from the Illumina sequencing data available in the lab. [score:3]
FIGURE 6 Time kinetics of fold change of Osa-miR820 and its target OsDRM2 under salt stress in leaf and root tissues of Pusa Basmati 1 (PB) and Pokkali (PK). [score:3]
This indicates the association of Osa-miR820:OsDRM2 regulation in pollen biology, which may in turn regulate the grain filling and/or grain quality in rice. [score:3]
Osa-miR820 Target Prediction and Validation. [score:3]
FIGURE 3 Cleavage site mapping of Osa-miR820 target DNA cytosine-5-methyltransferase (OsDRM2). [score:3]
The expression levels of mature miR820 were analyzed using stem-loop end point PCR with slight modifications (Varkonyi-Gasic et al., 2007). [score:3]
It was observed that though Osa-miR820 is present in all the tissues, its expression was abundant in the mature leaf and root tissues (Figure 4A). [score:3]
Kinetics of miR820 and Its Target Under Salt Stress. [score:3]
FIGURE 2 Relative expression patterns of Osa-miR820 length variants. [score:3]
To delineate the role of Osa-miR820 in the physiology of rice the expression levels of the miR and OsDRM2 transcripts were checked in different tissues of PB1 and PK (Figure 4). [score:3]
FIGURE 7 Time kinetics of Osa-miR820 and its target OsDRM2 under high temperature and drought stress. [score:3]
To identify the transcripts that are affected due to changes in expression levels of the Osa-miR820, three different in silico approaches were used. [score:3]
Therefore to understand the functional significance and putative interaction of Osa-miR820 and OsDRM2 during reproductive development and abiotic stress detailed transcript profiling was performed. [score:2]
It was observed that the Osa-miR820 expression was more in the early reproductive phases and late embryogenic callus as compared to the other vegetative tissues. [score:2]
In both rice and Arabidopsis leaves, the OsDRM2 levels are induced at 30 min of stress and then decrease at 1 h although OsDRM2 is regulated by Osa-miR820 in rice only. [score:2]
Under salt stress, the levels of Osa-miR820 did not change extensively though a reverse pattern of deregulation was obtained in PB1 and PK. [score:2]
The expression profiling in flag leaf revealed a greater accumulation of Osa-miR820 in flag leaf of PK as compared to PB1, though the levels declined under salt stress (Figure 5E). [score:2]
However, it is important to understand that the OsDRM2 transcript levels may also be influenced at the transcriptional level by the 24-nt Osa-miR820 species. [score:1]
Sequence Analysis of Osa-miR820 Family in Rice. [score:1]
Mapping miR820 on the Indica Genome. [score:1]
Osa-miR820 belongs to a family of three closely related members. [score:1]
In PK roots a high variation in the levels of Osa-miR820 and OsDRM2 was observed (Figure 7D). [score:1]
The levels of Osa-miR820 were similar between salt stressed PB1 and normal PK tissues in pre-and post-pollinated panicles (Figures 5C, D). [score:1]
Two loci corresponding to pre-miR820b and pre-miR820c were mapped on chromosome 7 and 10, respectively (Figure 1). [score:1]
The time kinetics of high temperature stress indicated an increase in the Osa-miR820 levels in the leaves of PB1 (Figure 7A) and PK (Figure 7C). [score:1]
Sequences of Osa-miR820 precursors were aligned with TIGR version 7 (Ouyang et al., 2007) and 93-11 genome (Zhao et al., 2004) to search all the loci of Osa-miR820 in japonica and indica sequences. [score:1]
However, the ratio of Osa-miR820:OsDRM2 was maintained at stages 1 and 2 and decreased slowly in stages 3 and 4 (Figure 4C). [score:1]
It was observed that in the leaves at 30 min of stress there is decrease in Osa-miR820 but it gradually increases by 24 h of stress (Figure 6A). [score:1]
Genomic locations of Osa-miR820 family members were identified by using Bowtie 1.0 (Langmead et al., 2009). [score:1]
The Osa-miR820 seems specific to rice, as homologs have not been detected in other plants. [score:1]
However, as the members of Osa-miR820 family share identical mature sequences it was difficult to predict which locus was active in these regions. [score:1]
In roots, low levels of Osa-miR820 were maintained throughout the stress (Figure 6B) but the levels of OsDRM2 begin to increase only at 24 h of stress period. [score:1]
The pre-miR820 sequences lie in a repeat rich region of genome flanked by CACTA type DNA retrotransposons on both ends. [score:1]
In PB1 roots, however, both Osa-miR820 and OsDRM2 levels were maintained at low levels under drought stress except at the 3 h time point (Figure 7F). [score:1]
Within the florets the ovary, lemma and awn had very low levels of Osa-miR820, though the OsDRM2 transcripts accumulated only in the ovary. [score:1]
The available information on Osa-miR820 family was used to search for homologs in the indica genome and the results are summarized in Table 1. As miR820 has not been mapped on the indica genome, we attempted to locate all the members on the Beijing indica 93-11 genome based on homology search. [score:1]
A time kinetics of 250 mM salt stress indicated an increase in the Osa-miR820 levels in the leaf (Figure 6A) and a decrease in the root tissues (Figure 6B). [score:1]
In PK roots, Osa-miR820 levels decreased gradually after 1 h of stress but OsDRM2 transcripts accumulated till 3 h beyond which they decreased drastically (Figure 7H). [score:1]
Digital signatures of Osa-miR820 expression for each dataset was calculated as transcripts per million (TPM) for comparisons across data sets. [score:1]
The reverse-correlation was captured in calli at 5 weeks post initiation, when the Osa-miR820 levels got enhanced while the OsDRM2 levels decreased. [score:1]
But in PK, a mild decrease in Osa-miR820 levels is accompanied by increase in OsDRM2 levels in salt stress. [score:1]
In the root tissues both Osa-miR820 and OsDRM2 decreased in PB1 although the Osa-miR820 levels increased during early stages of stress. [score:1]
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3
[+] score: 63
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, osa-MIR528, osa-MIR535, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR820a, osa-MIR820c, osa-MIR821a, osa-MIR821b, osa-MIR821c, osa-MIR1432, osa-MIR169r, osa-MIR1846d, osa-MIR1846a, osa-MIR1846b, osa-MIR1876, osa-MIR1846c, osa-MIR1846e, osa-MIR395x, osa-MIR395y
NAM domain containing proteins, putative miR820 Structural proteins LOC_Os09g33800.1 Arabinogalactan putative, expressed miR528 LOC_Os04g47580.1 Cyclin putative, expressed miR535 LOC_Os02g38340.1 Actin putative, expressed miR821 In order to find possible miRNA/target gene modules that are differentially regulated in leaves and roots of IC-547557 and Vivek Dhan under low-N condition, the expression profiles of some predicted target genes were examined by qRT-PCR (Figs. 5 and 6). [score:14]
NAM domain containing proteins, putative miR820 Structural proteins LOC_Os09g33800.1 Arabinogalactan putative, expressed miR528 LOC_Os04g47580.1 Cyclin putative, expressed miR535 LOC_Os02g38340.1 Actin putative, expressed miR821In order to find possible miRNA/target gene modules that are differentially regulated in leaves and roots of IC-547557 and Vivek Dhan under low-N condition, the expression profiles of some predicted target genes were examined by qRT-PCR (Figs. 5 and 6). [score:14]
Similarly, expression of genes of Ca [2+] transporting ATPase (miR1318 target), Os-SPL19-SBP box gene family member (miR156 target) and DNA methyltransferase (miR820 target) were higher in leaves of IC-547557 when compared with Vivek Dhan. [score:8]
The miR820 direct methylation around its target site, Os03g02010, and thereby regulate its function through methylation [64] the same gene was more expressed in IC-547557 than in Vivek Dhan. [score:7]
To validate the microarray results of genome wide analysis, nine differentially expressed miRNAs from miR156, miR164, miR166, miR167, miR168, miR528, miR820, miR821 and miR1318 families were selected for the validation of their expression levels. [score:5]
Since an analysis of target genes of miR169 and miR167 family had already been evaluated in earlier studies [17], [30], we selected few target genes from miR156, miR164, miR168, miR528, miR820 and miR1318 families to observe the expression pattern in low-N tolerant (IC-547557) and low-N sensitive (Vivek Dhan) rice genotypes under nitrogen limited condition. [score:5]
Some of the targets of miR820 family (miR820a, miR820b and miR820c) are the genes encoding DNA methyltransferase. [score:3]
Six miRNAs (miR156, miR164, miR528, miR820, miR821 and miR1318) showed differential expression in the leaves, when comparison was made between Vivek Dhan and IC-547557 genotypes of rice (Fig. 4A). [score:3]
Of these differentially expressed miRNAs, six miRNAs (miR156, miR164, miR528, miR820, miR821 and miR1318) were reported in leaves and four (miR164, miR167, miR168 and miR528) in roots. [score:3]
These miRNAs belong to miR156, miR164, miR166, miR167, miR168, miR169, miR528, miR535, miR820, miR821, miR1318, miR1432, miR1846, miR1876, and miR2123 families. [score:1]
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[+] score: 12
The targeting of miRNA820 to DRM2 is evolutionarily conserved in the Oryza genus and the repression of DRM2 gene results in strong reduction in DNA methylation and transcriptional upregulation of many TEs. [score:6]
Interestingly, miRNA820 targets the transcripts of DRM2 that encodes a de novo DNA methyltransferase (Figure 1A). [score:3]
One example is miRNA820 of rice. [score:1]
miRNA820 is 22 or 24 nt in size and is originated from the internal region of CACTA DNA TE (Nosaka et al., 2012). [score:1]
Therefore, miRNA820 can be seen as an anti-silencing factor encoded within a TE that works at the post-transcriptional level. [score:1]
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[+] score: 12
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR156k, osa-MIR156l, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR408, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, osa-MIR396e, osa-MIR444a, osa-MIR531a, osa-MIR810a, osa-MIR812a, osa-MIR812b, osa-MIR812c, osa-MIR812d, osa-MIR812e, osa-MIR820a, osa-MIR820c, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR810b, osa-MIR531b, osa-MIR1846d, osa-MIR1846a, osa-MIR1846b, osa-MIR1861a, osa-MIR1861b, osa-MIR1861c, osa-MIR1861d, osa-MIR1861e, osa-MIR1861f, osa-MIR1861g, osa-MIR1861h, osa-MIR1861i, osa-MIR1861j, osa-MIR1861k, osa-MIR1861l, osa-MIR1861m, osa-MIR1861n, osa-MIR1862a, osa-MIR1862b, osa-MIR1862c, osa-MIR812f, osa-MIR1874, osa-MIR1862d, osa-MIR1862e, osa-MIR812g, osa-MIR812h, osa-MIR812i, osa-MIR812j, osa-MIR1846c, osa-MIR1846e, osa-MIR396f, osa-MIR2103, osa-MIR2105, osa-MIR396g, osa-MIR396h, osa-MIR396d, osa-MIR812k, osa-MIR812l, osa-MIR812m, osa-MIR1862f, osa-MIR1862g, osa-MIR812n, osa-MIR812o, osa-MIR812p, osa-MIR812q, osa-MIR812r, osa-MIR812s, osa-MIR812t, osa-MIR812u, osa-MIR812v, osa-MIR1861o, osa-MIR531c
It is also worth mentioning that the non-conserved miRNA, osa-miR820, which showed high constitutive expression levels throughout grain filling, has been confirmed to target mRNAs encoding a DNA cytosine methyltransferase (Zmet3-like) (Os03g02010) [16] (Table S6), a key enzyme involved in DNA methylation, indicating that epigenetic regulation may be an important mechanism underlying seed development. [score:7]
There were a few exceptions; four rice-specific miRNAs, osa-miR1861, osa-miR1862, osa-miR812, and osa-miR820, displayed relatively high expression during grain filling, suggesting that they may be important regulators of rice grain development. [score:5]
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[+] score: 9
However, we found that osa-miR820 -mediated cleavage of Os03g02010 was predominantly at nucleotide 7 from the paired 3' end (5 of 14) from the mixed cDNA pool from developmental pollen, and no other degradation fragments were detected with the targeted sequence (data not show). [score:4]
Furthermore, our 5' RACE analysis, in combination with several previous reports [31, 32, 44], confirmed that osa-miR820, which is enriched in pollen, targeted a DNA cytosine methyltransferase gene (Os03g02010). [score:3]
The miRNAs represented by the positive bars (Figure 3b; including miR3, miR1, miR5, miR4, miR14, miR29 and osa-miR820, osa-miR1881, osa-miR1871, osa-miR1874-3p, osa-miR2106, and osa-miR810b. [score:1]
Furthermore, cleavage of the non-conserved kn-miR osa-miR820 occurred primarily at the canonical position with a frequency of nine out of ten in a cDNA pool from seedling and inflorescence [31] or seven out of eight in a mixture of cDNA from panicle and embryogenic calli [32] but infrequently (one of ten) between nucleotides 11 and 12 from the complementary 5' end of osa-miR820 [31]. [score:1]
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[+] score: 7
Epigenetic regulation of chromatin assembly and disassembly might be controlled by the pollen development through over -expression of osa-miR820 and osa-miR827, targeted Os03g02010 (DNA cytosine methyl transferase) and Os04g11510 (a methyl-CpG binding domain protein), respectively. [score:7]
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[+] score: 4
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR162a, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR394, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR319a, osa-MIR319b, osa-MIR160e, osa-MIR160f, osa-MIR162b, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR408, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, osa-MIR413, osa-MIR414, osa-MIR415, osa-MIR416, osa-MIR417, osa-MIR418, osa-MIR419, osa-MIR426, osa-MIR390, osa-MIR396e, osa-MIR444a, osa-MIR530, osa-MIR535, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR818a, osa-MIR818b, osa-MIR818c, osa-MIR818d, osa-MIR818e, osa-MIR820a, osa-MIR820c, osa-MIR1423, osa-MIR1425, osa-MIR1427, osa-MIR1428a, osa-MIR1429, osa-MIR1430, osa-MIR1431, osa-MIR1432, osa-MIR169r, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR810b, osa-MIR1435, osa-MIR1436, osa-MIR1437a, osa-MIR1440a, osa-MIR1441, osa-MIR1442, osa-MIR1439, osa-MIR1428b, osa-MIR1428c, osa-MIR1428d, osa-MIR1428e, osa-MIR1428f, osa-MIR1428g, osa-MIR396f, osa-MIR396g, osa-MIR396h, osa-MIR396d, osa-MIR395x, osa-MIR395y, osa-MIR1440b, osa-MIR818f, osa-MIR1437b
The expression analysis also included Osa-miR820, a known miRNA in rice. [score:3]
Among the known non-conserved rice miRNAs, miR820, miR535, miR818 and miR530 appeared 20 times or more, at least in one of the 3 libraries (Table 4). [score:1]
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[+] score: 4
High-throughput sequencing of miRNAs showed that 14 rice miRNA families (osa-miR156, miR160, miR164, miR166, miR167, miR168, miR171, miR319, miR396, miR397, miR408, miR528, miR530, miR820) were significantly down-regulated after drought treatment [23]. [score:4]
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[+] score: 1
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR169a, osa-MIR393a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR393b, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, osa-MIR444a, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR166l, zma-MIR166m, zma-MIR393a, zma-MIR156k, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR820a, osa-MIR820c, osa-MIR1425, osa-MIR1428a, osa-MIR169r, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR1428b, osa-MIR1428c, osa-MIR1428d, osa-MIR1428e, osa-MIR1874, osa-MIR2055, osa-MIR827, osa-MIR1428f, osa-MIR1428g, zma-MIR396d, osa-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR827, osa-MIR395x, osa-MIR395y, zma-MIR444a, zma-MIR444b
A few of them which are abundant are regularly cloned, such as osa-miR444 or osa-miR820, that we also cloned. [score:1]
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11
[+] score: 1
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR171a, osa-MIR393a, osa-MIR394, osa-MIR395f, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR398b, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR319a, osa-MIR319b, osa-MIR160f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR393b, osa-MIR408, osa-MIR172d, osa-MIR171i, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, osa-MIR390, osa-MIR396e, osa-MIR444a, osa-MIR528, osa-MIR529a, osa-MIR810a, osa-MIR812a, osa-MIR812b, osa-MIR812c, osa-MIR812d, osa-MIR812e, osa-MIR818a, osa-MIR818b, osa-MIR818c, osa-MIR818d, osa-MIR818e, osa-MIR820a, osa-MIR820c, osa-MIR529b, osa-MIR1425, osa-MIR1430, osa-MIR1432, osa-MIR169r, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR810b, osa-MIR1440a, osa-MIR531b, osa-MIR1847, osa-MIR1848, osa-MIR1861a, osa-MIR1861b, osa-MIR1861c, osa-MIR1861d, osa-MIR1861e, osa-MIR1861f, osa-MIR1861g, osa-MIR1861h, osa-MIR1861i, osa-MIR1861j, osa-MIR1861k, osa-MIR1861l, osa-MIR1861m, osa-MIR1861n, osa-MIR1865, osa-MIR812f, osa-MIR1874, osa-MIR812g, osa-MIR812h, osa-MIR812i, osa-MIR812j, osa-MIR1320, osa-MIR827, osa-MIR2090, osa-MIR396f, osa-MIR2118c, osa-MIR2863a, osa-MIR2863b, osa-MIR396g, osa-MIR396h, osa-MIR396d, osa-MIR812k, osa-MIR812l, osa-MIR812m, osa-MIR3979, osa-MIR3980a, osa-MIR3980b, osa-MIR812n, osa-MIR812o, osa-MIR3981, osa-MIR5082, osa-MIR2863c, osa-MIR5337a, osa-MIR812p, osa-MIR812q, osa-MIR812r, osa-MIR812s, osa-MIR812t, osa-MIR812u, osa-MIR812v, osa-MIR1440b, osa-MIR818f, osa-MIR1861o
The miR393, miR396 and miR820 responded to the saline and alkaline stress [52]. [score:1]
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